Human SLC27A5 ELISA Kit (V2LY-0626-LY2609)
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Basic Information
- Pre-coated ELISA Plate: 12 wells * 8 detachable strips
- Standard solution: 0.5ml x1
- Standard diluent: 3ml x1
- Streptavidin-HRP: 6ml x1
- Stop solution: 6ml x1
- Substrate solution A: 6ml x1
- Substrate solution B: 6ml x1
- Wash buffer concentrate (25x): 20ml x1
- Biotinylated antibody: 1ml x1
Formulations & Storage [For reference only, actual COA shall prevail!]
Target
Also catalyzes the ATP-dependent formation of fatty acyl-CoA using LCFA and very-long-chain fatty acids (VLCFA) as substrates (PubMed:10479480).
Mainly functions as a bile acyl-CoA synthetase catalyzing the activation of bile acids via ATP-dependent formation of bile acid CoA thioesters which is necessary for their subsequent conjugation with glycine or taurine (PubMed:10749848, PubMed:11980911).
Both primary bile acids (cholic acid and chenodeoxycholic acid) and secondary bile acids (deoxycholic acid and lithocholic acid) are the principal substrates (PubMed:10749848, PubMed:11980911).
In vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate ((25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate or THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol (PubMed:11980911).
Plays an important role in hepatic fatty acid uptake and bile acid reconjugation and recycling but not in de novo synthesis of bile acids (By similarity).
Biological Process bile acid biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process establishment of localization in cellIEA:Ensembl
Biological Process ketone body biosynthetic processManual Assertion Based On ExperimentIBA:GO_Central
Biological Process long-chain fatty acid import across plasma membraneManual Assertion Based On ExperimentIBA:GO_Central
Biological Process triglyceride mobilizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process very long-chain fatty acid metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Microsome
Cell membrane
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
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Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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