Rat Atp5f1c ELISA Kit (V2LY-0626-LY2074)

Name: Rat Atp5f1c ELISA Kit
SKU: V2LY-0626-LY2074
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0091 ng/mL

Detection Range

0.02-4.5 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Rat

Assay Type

Sandwich

Reactivity

Rat

Assay Time

1.5 h

Molecule Mass

30.2 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

ATP Synthase F1 Subunit Gamma

Function

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha3beta3. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Biological Process

ATP biosynthetic process Source: Reactome
ATP synthesis coupled proton transport Source: GO_Central
Cristae formation Source: Reactome
Mitochondrial ATP synthesis coupled proton transport Source: UniProtKB
Oxidative phosphorylation Source: UniProtKB

Cellular Location

Mitochondrion inner membrane

Fiorillo, M., Scatena, C., Naccarato, A. G., Sotgia, F., & Lisanti, M. P. (2021). Bedaquiline, an FDA-approved drug, inhibits mitochondrial ATP production and metastasis in vivo, by targeting the gamma subunit (ATP5F1C) of the ATP synthase. Cell Death & Differentiation, 1-21.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human ATP5F1C ELISA Kit (CAT#: V2LY-0626-LY5653)

Related Products

Mouse Anti-ATP5F1C Recombinant Antibody (CF041) (CAT#: CBMAB-FT430LY)

Human ATP5F1C ELISA Kit (CAT#: V2LY-0626-LY5653)

Mouse Anti-ATP5F1C Recombinant Antibody (8E1) (CAT#: CBMAB-AP490LY)

Mouse Anti-ATP5F1C Recombinant Antibody (2H6) (CAT#: CBMAB-A4059-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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