Human TPP1 ELISA Kit (V2LY-0626-LY3139)

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Tested Data
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Basic Information

Sensitivity
0.0032 ng/mL
Detection Range
0.007-1.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
61.2 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
TPP1 Gene(Protein Coding) Tripeptidyl Peptidase 1
Function
Lysosomal serine protease with tripeptidyl-peptidase I activity (PubMed:11054422, PubMed:19038966, PubMed:19038967).
May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases (PubMed:11054422, PubMed:19038966, PubMed:19038967).
Requires substrates with an unsubstituted N-terminus (PubMed:19038966).
Biological Process
Biological Process bone resorption Source:UniProtKB1 Publication
Biological Process central nervous system development Source:GO_Central1 Publication
Biological Process epithelial cell differentiation Source:UniProtKB1 Publication
Biological Process lipid metabolic process Source:ProtInc1 Publication
Biological Process lysosomal protein catabolic process Source:Ensembl
Biological Process lysosome organization Source:UniProtKB
Biological Process nervous system development Source:UniProtKB1 Publication
Biological Process neuromuscular process controlling balance Source:UniProtKB
Biological Process peptide catabolic process Source:UniProtKB2 Publications
Biological Process protein catabolic process Source:UniProtKB1 Publication
Biological Process protein localization to chromosome, telomeric region Source:CACAO1 Publication
Biological Process proteolysis Source:UniProtKB2 Publications
Cellular Location
Lysosome
Melanosome
Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Involvement in disease
Ceroid lipofuscinosis, neuronal, 2 (CLN2):
A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN2 consists of curvilinear profiles.
Spinocerebellar ataxia, autosomal recessive, 7 (SCAR7):
A form of spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR7 patients show difficulty walking and writing, dysarthria, limb ataxia, and cerebellar atrophy.
PTM
Activated by autocatalytic proteolytical processing upon acidification (PubMed:11054422, PubMed:19038966, PubMed:19038967).
N-glycosylation is required for processing and activity (PubMed:19038966, PubMed:19038967).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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