Human TRPV5 ELISA Kit (V2LY-0626-LY5354)
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Basic Information
- Pre-coated ELISA plate: 12 wells * 8 detachable strips
- Standard solution: 0.5ml x1
- Standard diluent: 3ml x1
- Streptavidin-HRP: 6ml x1
- Stop solution: 6ml x1
- Substrate solution A: 6ml x1
- Substrate solution B: 6ml x1
- Wash buffer concentrate (25x): 20ml x1
- Biotinylated antibody: 1ml x1
Formulations & Storage [For reference only, actual COA shall prevail!]
Target
Required for normal Ca2+ reabsorption in the kidney distal convoluted tubules (By similarity).
The channel is activated by low internal calcium level and the current exhibits an inward rectification (PubMed:11549322, PubMed:18768590).
A Ca2+-dependent feedback regulation includes fast channel inactivation and slow current decay (By similarity).
Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).
Biological Process calcium ion import across plasma membrane Source:UniProtKB1 Publication
Biological Process calcium ion transmembrane transport Source:UniProtKB1 Publication
Biological Process calcium ion transport Source:UniProtKB
Biological Process protein homotetramerization Source:UniProtKB
Biological Process regulation of urine volume Source:UniProtKB
Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells (By similarity).
The expression of the glycosylated form in the cell membrane is increased in the presence of WNK3 (PubMed:18768590).
Helical: 328-348
Extracellular: 349-385
Helical: 386-408
Cytoplasmic: 409-419
Helical: 420-442
Extracellular: 443-448
Helical: 449-469
Cytoplasmic: 470-492
Helical: 493-513
Pore-forming: 524-544
Helical: 557-577
Cytoplasmic: 578-729
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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