Human VAMP8 ELISA Kit (V2LY-0626-LY5422)

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Tested Data
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Basic Information

Sensitivity
0.009 ng/mL
Detection Range
0.02-7 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
11.4 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Vesicle Associated Membrane Protein 8
Function
SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed:23217709, PubMed:25686604).
Also required for dense-granule secretion in platelets (PubMed:12130530).
Also plays a role in regulated enzyme secretion in pancreatic acinar cells (By similarity).
Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity).
Involved in the homotypic fusion of early and late endosomes (By similarity).
Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism (PubMed:31694946).
Biological Process
Biological Process autophagosome maturation Source:UniProtKB1 Publication
Biological Process autophagosome membrane docking Source:GO_Central1 Publication
Biological Process defense response to virus Source:UniProtKB-KW
Biological Process mucus secretion Source:UniProtKB1 Publication
Biological Process negative regulation of secretion by cell Source:UniProtKB1 Publication
Biological Process positive regulation of histamine secretion by mast cell Source:UniProtKB1 Publication
Biological Process protein transport Source:UniProtKB-KW
Biological Process regulation of protein localization to plasma membrane Source:UniProtKB1 Publication
Biological Process SNARE complex assembly Source:GO_Central1 Publication
Biological Process vesicle fusion Source:GO_Central1 Publication
Biological Process viral entry into host cell Source:MGI1 Publication
Cellular Location
Lysosome membrane
Early endosome membrane
Late endosome membrane
Cell membrane
Zymogen granule membrane
Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (By similarity).
Sub-tight junctional domain in retinal pigment epithelium cells. Midbody region during cytokinesis. Lumenal oriented, apical membranes of nephric tubular cell (By similarity).
Cycles through the apical but not through the basolateral plasma membrane (By similarity).
Apical region of acinar cells; in zymogen granule membranes (By similarity).
Topology
Cytoplasmic: 1-75
Helical: 76-96
Vesicular: 97-100
PTM
(Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, thereby disrupting the host actin cytoskeleton.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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