Human WNK2 ELISA Kit (V2LY-0626-LY5227)

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Tested Data
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Basic Information

Sensitivity
0.0075 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
242.7 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
WNK lysine deficient protein kinase 2
Function
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.
Biological Process
Biological Process intracellular signal transduction Source:UniProtKB
Biological Process ion homeostasis Source:UniProtKB1 Publication
Biological Process negative regulation of cell population proliferation Source:UniProtKB1 Publication
Biological Process negative regulation of ERK1 and ERK2 cascade Source:UniProtKB1 Publication
Biological Process negative regulation of sodium ion transport Source:GO_Central1 Publication
Biological Process positive regulation of canonical Wnt signaling pathway Source:FlyBase1 Publication
Biological Process positive regulation of potassium ion import across plasma membrane Source:GO_Central1 Publication
Biological Process positive regulation of sodium ion transmembrane transporter activity Source:UniProtKB1 Publication
Biological Process protein autophosphorylation Source:UniProtKB1 Publication
Biological Process protein phosphorylation Source:UniProtKB1 Publication
Cellular Location
Cytoplasm
Cell membrane
PTM
Autophosphorylated.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant WNK2, Active protein, GST Tag (CAT#: V2LY-0526-LY7674)

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Human Recombinant WNK2, Active protein, GST Tag (CAT#: V2LY-0526-LY7674)

Mouse Anti-WNK2 Recombinant Antibody (2A2) (CAT#: CBMAB-W0062-WJ)

Mouse Anti-WNK2 Recombinant Antibody (2E10) (CAT#: CBMAB-W0073-WJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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