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Canine Interleukin Antibody Array (Membrane, 11 Targets) (Y0552) (AbAr-0552-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The product provided herein is a Canine Interleukin Antibody Array, which can be used for semi-quantitative detection of 11 canine interleukin proteins. It is suitable for a range of liquid forms including Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates.
See all Interleukin antibodies

Specifications

Target Array
Interleukin Array
Target
IL-1 alpha(IL-1 F1), IL-1 beta(IL-1 F2), IL-1 Ra(IL-1 F3), IL-10, IL-12 p40, IL-13, IL-17A, IL-2, IL-21, IL-6, IL-8(CXCL8)
Species Reactivity
Canine
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Block the array membrane surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Analyze data
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
Interleukins (ILs) are a group of cytokines (secreted proteins and signal molecules) that were first seen to be expressed by white blood cells (leukocytes). ILs can be divided into four major groups based on distinguishing structural features. However, their amino acid sequence similarity is rather weak (typically 15–25% identity). The human genome encodes more than 50 interleukins and related proteins. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4 T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T and B lymphocytes, and hematopoietic cells.
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Alternative Versions

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0557)

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0555)

Porcine Interleukin Antibody Array (Membrane, 16 Targets) (Y0554)

Bovine Interleukin Antibody Array (Membrane, 11 Targets) (Y0553)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0556)

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0118)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0117)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0116)

Antibody Array

Bovine Interleukin Antibody Array (Membrane, 11 Targets) (Y0553)

Porcine Interleukin Antibody Array (Membrane, 16 Targets) (Y0554)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0116)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0117)

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0118)

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0555)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0556)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0557)

Related Products

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0118)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0116)

Porcine Interleukin Antibody Array (Membrane, 16 Targets) (Y0554)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0117)

Bovine Interleukin Antibody Array (Membrane, 11 Targets) (Y0553)

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0555)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0556)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0557)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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