Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0118) (AbAr-0118-YC)

Name: Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0118)
SKU: AbAr-0118-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Specifications

Target Array

Interleukin Array

Target

IL-1 ra, IL-17B, IL-2 R alpha, IL-17E (IL-25), IL-6 R, IL-17F, IL-11, IL-20, IL-12 p40, IL-28A

Species Reactivity

Mouse

Sample Type

Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates, Other Body Fluids

Applications

Application

Multiplex Protein Detection

Assay Type

Quantitative

Solid Support

Glass Slide

Detection Assay

Sandwich-based

Protocol Outline

Thoroughly dry the glass slide and block the array surface to prevent non-specific binding.
Incubate the array with both samples and standards.
Incubate with a biotinylated detection antibody cocktail and then with streptavidin-conjugated fluorescent dye.
Carefully disassemble the glass slide and scan the slide using a gene microarray laser scanner.
Conduct densitometric analysis.

Components

Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction

Interleukins (ILs) are a group of cytokines (secreted proteins and signal molecules) that were first seen to be expressed by white blood cells (leukocytes). ILs can be divided into four major groups based on distinguishing structural features. However, their amino acid sequence similarity is rather weak (typically 15–25% identity). The human genome encodes more than 50 interleukins and related proteins. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4 T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T and B lymphocytes, and hematopoietic cells.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Canine Interleukin Antibody Array (Membrane, 11 Targets) (Y0552) (CAT#: AbAr-0552-YC)

Bovine Interleukin Antibody Array (Membrane, 11 Targets) (Y0553) (CAT#: AbAr-0553-YC)

Porcine Interleukin Antibody Array (Membrane, 16 Targets) (Y0554) (CAT#: AbAr-0554-YC)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0116) (CAT#: AbAr-0116-YC)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0117) (CAT#: AbAr-0117-YC)

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0555) (CAT#: AbAr-0555-YC)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0556) (CAT#: AbAr-0556-YC)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0557) (CAT#: AbAr-0557-YC)

Antibody Array

Canine Interleukin Antibody Array (Membrane, 11 Targets) (Y0552) (CAT#: AbAr-0552-YC)

Bovine Interleukin Antibody Array (Membrane, 11 Targets) (Y0553) (CAT#: AbAr-0553-YC)

Porcine Interleukin Antibody Array (Membrane, 16 Targets) (Y0554) (CAT#: AbAr-0554-YC)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0116) (CAT#: AbAr-0116-YC)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0117) (CAT#: AbAr-0117-YC)

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0555) (CAT#: AbAr-0555-YC)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0556) (CAT#: AbAr-0556-YC)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0557) (CAT#: AbAr-0557-YC)

Related Products

Porcine Interleukin Antibody Array (Membrane, 16 Targets) (Y0554) (CAT#: AbAr-0554-YC)

Canine Interleukin Antibody Array (Membrane, 11 Targets) (Y0552) (CAT#: AbAr-0552-YC)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0557) (CAT#: AbAr-0557-YC)

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0555) (CAT#: AbAr-0555-YC)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0117) (CAT#: AbAr-0117-YC)

Bovine Interleukin Antibody Array (Membrane, 11 Targets) (Y0553) (CAT#: AbAr-0553-YC)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0556) (CAT#: AbAr-0556-YC)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0116) (CAT#: AbAr-0116-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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