Rat L90 Antibody Array (Glass Slide, 90 Targets) (Y0076) (AbAr-0076-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Rat L90 Antibody Array detects 90 Rat Proteins. It is suitable for serum, plasma, and cell culture supernatants.

Datasheet

SDS

Specifications

Target Array

L90 Array

Target

Activin A, ACTH, ADFP, Adiponectin, AMPK alpha 1, B7-1/CD80, BDNF, beta-Catenin, bFGF, b-NGF, CCR4, CD106, CINC-2 alpha/beta, CINC-3, CNTF, CNTF R alpha, CSK, CXCR4, EGFR, EG-VEGF/PK1, E-Selectin, FADD, Fas Ligand/TNFSF6, Fas/TNFRSF6, FGF-BP, Follostatin-like -1 (FSL1), Fractalkine, GFR alpha-1, GFR alpha-2, GM-CSF, Growth Hormone, Growth Hormone R, Hepassocin, ICAM-1/CD54, ICK, Insulin Degrading Enzyme (IDE), IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 R6/IL-1 R rp2, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12/IL-23 p40, IL-13, Integrin alpha M beta 2, Inuslin, IP-10, Leptin (OB), LIX, L-Selectin/CD62L, MCP-1, MDC, MIF, MIP-1 alpha, MIP-2, MIP-3 alpha, MMP-13, MMP-2, MMP-8, MuSK, Neuropilin-2, NGFR, Orexin A, Osteopontin/SPP1, PDGF-AA, Prolactin R, RAGE, RALT/MIG-6, RELM beta, Resistin, TL1A/TNFSF15/VEGI, TGF beta 1, TGF beta 2, TGF beta 3, Thrombospondin, TIE-2, TIMP-1, TIMP-2, TIMP-3, TLR4, TNF alpha, TRAIL, TROY, Ubiquitin+1, VEGF-A, VEGF-C

Species Reactivity

Rat

Sample Type

Serum, Plasma, Cell Culture Supernatant

Applications

Application

Multiplex Protein Detection

Assay Type

Semi-Quantitative

Solid Support

Glass Slide

Detection Assay

Label-based

Protocol Outline

Dialyze samples in PBS
Biotinylate Samples
Dialyze biotinylated samples using spin column
Dry the glass slide
Block array surface
Incubate with Samples
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Components

Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

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