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Rat Protein Antibody Array (Glass Slide, 90 Targets) (Y0076) (AbAr-0076-YC)


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Specifications

Target Array
Rat Protein Array
Target
Activin A, ACTH, ADFP, Adiponectin, AMPK alpha 1, CD80, BDNF, beta-Catenin, bFGF, b-NGF, CCR4, CD106, CINC-2 alpha/beta, CINC-3, CNTF, CNTF R alpha, CSK, CXCR4, EGFR, EG-VEGF, E-Selectin, FADD, Fas Ligand/TNFSF6, TNFRSF6, FGF-BP, FSL1, Fractalkine, GFR alpha-1, GFR alpha-2, GM-CSF, Growth Hormone, Growth Hormone R, Hepassocin, ICAM-1, ICK, Insulin Degrading Enzyme (IDE), IFNG, IL-1 alpha, IL-1 beta, IL-1 R6/IL-1 R rp2, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12/IL-23 p40, IL-13, Integrin alpha M beta 2, Inuslin, IP-10, Leptin (OB), LIX, L-Selectin/CD62L, MCP-1, MDC, MIF, CCL3, CXCL2, CCL20, MMP-13, MMP-2, MMP-8, MuSK, NRP2, NGFR, Orexin A, Osteopontin/SPP1, PDGF-AA, Prolactin R, RAGE, RALT/MIG-6, RELM beta, Resistin, TL1A/TNFSF15/VEGI, TGF beta 1, TGF beta 2, TGF beta 3, Thrombospondin, TIE-2, TIMP-1, TIMP-2, TIMP-3, TLR4, TNF alpha, TRAIL, TROY, Ubiquitin+1, VEGF-A, VEGF-C
Species Reactivity
Rat
Sample Type
Serum, Plasma, Cell Culture Supernatant

Applications

Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Label-based
Protocol Outline
Biotinylate the samples and dialyze the biotinylated samples in PBS.
Allow the glass slide to dry thoroughly.
Block the surface of the array.
Incubate the array with the samples.
Incubate with streptavidin-conjugated fluorescent dye.
Carefully disassemble the glass slide.
Scan the slides using a microarray laser scanner.
Conduct densitometric analysis and interpret the data.
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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