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Rat Protein Antibody Array (Membrane, 90 Targets) (Y0077) (AbAr-0077-YC)


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Specifications

Target Array
Rat Protein Array
Target
Activin A, ACTH, ADFP, Adiponectin, AMPK alpha 1, CD80, BDNF, beta-Catenin, bFGF, b-NGF, CCR4, CD106, CINC-2 alpha/beta, CINC-3, CNTF, CNTF R alpha, CSK, CXCR4, EGFR, EG-VEGF, E-Selectin, FADD, Fas Ligand/TNFSF6, TNFRSF6, FGF-BP, FSL1, Fractalkine, GFR alpha-1, GFR alpha-2, GM-CSF, Growth Hormone, Growth Hormone R, Hepassocin, ICAM-1, ICK, Insulin Degrading Enzyme (IDE), IFNG, IL-1 alpha, IL-1 beta, IL-1 R6/IL-1 R rp2, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12/IL-23 p40, IL-13, Integrin alpha M beta 2, Inuslin, IP-10, Leptin (OB), LIX, L-Selectin/CD62L, MCP-1, MDC, MIF, CCL3, CXCL2, CCL20, MMP-13, MMP-2, MMP-8, MuSK, NRP2, NGFR, Orexin A, Osteopontin/SPP1, PDGF-AA, Prolactin R, RAGE, RALT/MIG-6, RELM beta, Resistin, TL1A/TNFSF15/VEGI, TGF beta 1, TGF beta 2, TGF beta 3, Thrombospondin, TIE-2, TIMP-1, TIMP-2, TIMP-3, TLR4, TNF alpha, TRAIL, TROY, Ubiquitin+1, VEGF-A, VEGF-C
Species Reactivity
Rat
Sample Type
Serum, Plasma, Cell Culture Supernatant

Applications

Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Label-based
Protocol Outline
Biotinylate the samples and dialyze the biotinylated samples in PBS.
Perform blocking of the array membranes.
Incubate the membranes with the samples.
Incubate with HRP-streptavidin.
Proceed with incubation using detection buffers.
Capture images with a chemiluminescent imaging system.
Conduct densitometric analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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