Rat Protein Antibody Array (Membrane, 90 Targets) (Y0077) (AbAr-0077-YC)

Name: Rat Protein Antibody Array (Membrane, 90 Targets) (Y0077)
SKU: AbAr-0077-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Q & As Review & reward Protocols Associated Products

Specifications

Target Array

Rat Protein Array

Target

Activin A, ACTH, ADFP, Adiponectin, AMPK alpha 1, CD80, BDNF, beta-Catenin, bFGF, b-NGF, CCR4, CD106, CINC-2 alpha/beta, CINC-3, CNTF, CNTF R alpha, CSK, CXCR4, EGFR, EG-VEGF, E-Selectin, FADD, Fas Ligand/TNFSF6, TNFRSF6, FGF-BP, FSL1, Fractalkine, GFR alpha-1, GFR alpha-2, GM-CSF, Growth Hormone, Growth Hormone R, Hepassocin, ICAM-1, ICK, Insulin Degrading Enzyme (IDE), IFNG, IL-1 alpha, IL-1 beta, IL-1 R6/IL-1 R rp2, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12/IL-23 p40, IL-13, Integrin alpha M beta 2, Inuslin, IP-10, Leptin (OB), LIX, L-Selectin/CD62L, MCP-1, MDC, MIF, CCL3, CXCL2, CCL20, MMP-13, MMP-2, MMP-8, MuSK, NRP2, NGFR, Orexin A, Osteopontin/SPP1, PDGF-AA, Prolactin R, RAGE, RALT/MIG-6, RELM beta, Resistin, TL1A/TNFSF15/VEGI, TGF beta 1, TGF beta 2, TGF beta 3, Thrombospondin, TIE-2, TIMP-1, TIMP-2, TIMP-3, TLR4, TNF alpha, TRAIL, TROY, Ubiquitin+1, VEGF-A, VEGF-C

Species Reactivity

Rat

Sample Type

Serum, Plasma, Cell Culture Supernatant

Applications

Application

Multiplex Protein Detection

Assay Type

Semi-Quantitative

Solid Support

Membrane

Detection Assay

Label-based

Protocol Outline

Biotinylate the samples and dialyze the biotinylated samples in PBS.
Perform blocking of the array membranes.
Incubate the membranes with the samples.
Incubate with HRP-streptavidin.
Proceed with incubation using detection buffers.
Capture images with a chemiluminescent imaging system.
Conduct densitometric analysis.

Components

Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Rat Protein Antibody Array (Glass Slide, 90 Targets) (Y0076) (CAT#: AbAr-0076-YC)

Antibody Array

Rat Protein Antibody Array (Glass Slide, 90 Targets) (Y0076) (CAT#: AbAr-0076-YC)

Related Products

Rat Protein Antibody Array (Glass Slide, 90 Targets) (Y0076) (CAT#: AbAr-0076-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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