Mouse Anti-AKR1C1 Recombinant Antibody (V2-611087) (CBMAB-A0240-LY)

Name: Mouse Anti-AKR1C1 Recombinant Antibody (V2-611087)
SKU: CBMAB-A0240-LY
Rating: 5 (1 reviews)

See all AKR1C1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-611087

Application

WB, ELISA

Immunogen

AKR1C1 (AAH20216, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Aldo-Keto Reductase Family 1 Member C1

Introduction

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq]

Entrez Gene ID

1645

UniProt ID

Q04828

Alternative Names

2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1; DDH; DDH1; H-37; HAKRC; MBAB; MGC8954

Function

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids (PubMed:19218247). Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH (PubMed:14672942). Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens (PubMed:10998348). May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate (PubMed:19218247). Displays affinity for bile acids (PubMed:8486699).

Biological Process

Bile acid and bile salt transport Source: UniProtKB
Bile acid metabolic process Source: UniProtKB
Cellular response to jasmonic acid stimulus Source: UniProtKB
Cholesterol homeostasis Source: UniProtKB
Daunorubicin metabolic process Source: UniProtKB
Digestion Source: UniProtKB
Doxorubicin metabolic process Source: UniProtKB
Epithelial cell differentiation Source: UniProtKB
Intestinal cholesterol absorption Source: UniProtKB
Progesterone metabolic process Source: UniProtKB
Prostaglandin metabolic process Source: GO_Central
Response to organophosphorus Source: UniProtKB
Retinal metabolic process Source: UniProtKB
Retinoid metabolic process Source: Reactome
Steroid metabolic process Source: GO_Central
Xenobiotic metabolic process

Cellular Location

Cytosol

Liu, X., Lian, X., Liu, X., Du, Y., Zhu, Y., Hu, M., ... & Zhou, Y. (2021). Aldo-keto reductase family 1 member C1 regulates the osteogenic differentiation of human ASCs by targeting the progesterone receptor. Stem cell research & therapy, 12(1), 1-13.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-AKR1C1 Recombinant Antibody (V2-611087)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-AKR1C1 Antibody (V2-2452) (CAT#: CBMAB-1004CQ)

Mouse Anti-AKR1C1 Recombinant Antibody (V2-180476) (CAT#: CBMAB-A1985-YC)

Mouse Anti-AKR1C1 Recombinant Antibody (CBT3982) (CAT#: V2LY-0625-LY2027)

Mouse Anti-AKR1C1 Recombinant Antibody (CBT3929) (CAT#: V2LY-0625-LY2026)

Mouse Anti-AKR1C1 Recombinant Antibody (V2-31915) (CAT#: CBMAB-D0005-YC)

Mouse Anti-AKR1C1 Recombinant Antibody (V2-180475) (CAT#: CBMAB-A1983-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry