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Mouse Anti-AKT3 Recombinant Antibody (V2-180582) (CBMAB-A2116-YC)

Provided herein is a Mouse monoclonal antibody against Human AKT Serine/Threonine Kinase 3. The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all AKT3 antibodies
Published Data
Fig.1 Akt-3 immunohistochemistry (IHC) in triple negative (TN) breast cancer and ER-positive breast cancer cases.
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Fig.2 Immunoblotting showing expression of Akt isoforms in a panel of breast cancer lines.
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Fig.3 MDA-MB-231 cells expressing tet-on Akt isoform shRNA were treated with dox for 3 days.
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Fig.4 Immunoblotting showing knockdown of Akt isoforms in tet-on MCF10ADCIS cells when treated with dox for 3 days.
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Fig.5 p27 is a downstream effector of Akt3 in regulating tumor spheroid growth.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180582
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
AKT3 (AAD29089, 100-189 aa) partial recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)10 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 100-189

Target

Full Name
AKT Serine/Threonine Kinase 3
Introduction
AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including
Entrez Gene ID
10000
UniProt ID
Q9Y243
Alternative Names
AKT Serine/Threonine Kinase 3; RAC-PK-Gamma; EC 2.7.11.1; PKB Gamma; STK-2; PKBG; V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma); V-Akt Murine Thymoma Viral Oncogene Homolog 3; RAC-Gamma Serine/Threonine Protein Kinase; RAC-Gamma
Function
AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
Biological Process
Brain morphogenesis Source: Ensembl
Homeostasis of number of cells within a tissue Source: Ensembl
Intracellular signal transduction Source: GO_Central
Mitochondrial genome maintenance Source: UniProtKB
Negative regulation of cellular senescence Source: BHF-UCL
Peptidyl-serine phosphorylation Source: GO_Central
Positive regulation of angiogenesis Source: Ensembl
Positive regulation of artery morphogenesis Source: Ensembl
Positive regulation of blood vessel endothelial cell migration Source: BHF-UCL
Positive regulation of cell migration involved in sprouting angiogenesis Source: BHF-UCL
Positive regulation of cell size Source: Ensembl
Positive regulation of endothelial cell proliferation Source: BHF-UCL
Positive regulation of TOR signaling Source: Ensembl
Positive regulation of vascular endothelial cell proliferation Source: BHF-UCL
Protein phosphorylation Source: ProtInc
Signal transduction Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus; Membrane. Membrane-associated after cell stimulation leading to its translocation.
Involvement in disease
AKT3 is a key modulator of several tumors like melanoma, glioma and ovarian cancer. Active AKT3 increases progressively during melanoma tumor progression with highest levels present in advanced-stage metastatic melanomas. Promotes melanoma tumorigenesis by decreasing apoptosis. Plays a key role in the genesis of ovarian cancers through modulation of G2/M phase transition. With AKT2, plays a pivotal role in the biology of glioblastoma.
Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 2 (MPPH2): A syndrome characterized by megalencephaly, hydrocephalus, and polymicrogyria; polydactyly may also be seen. There is considerable phenotypic similarity between this disorder and the megalencephaly-capillary malformation syndrome.
PTM
Phosphorylation on Thr-305 and Ser-472 is required for full activity.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
O-GlcNAcylation at Thr-302 and Thr-309 inhibits activating phosphorylation at Thr-305 via disrupting the interaction between AKT and PDK1.

Shi, F., Yang, Q., Shen, D., & Chen, J. (2021). CircRNA WHSC1 promotes non‐small cell lung cancer progression via sponging microRNA‐296‐3p and up‐regulating expression of AKT serine/threonine kinase 3. Journal of clinical laboratory analysis, e23865.

Hinz, N., Baranowsky, A., Horn, M., Kriegs, M., Sibbertsen, F., Smit, D. J., ... & Jücker, M. (2021). Knockdown of AKT3 Activates HER2 and DDR Kinases in Bone-Seeking Breast Cancer Cells, Promotes Metastasis In Vivo and Attenuates the TGFβ/CTGF Axis. Cells, 10(2), 430.

Srivastava, S., Mehta, P., Sharma, O., Sharma, M., & Malik, R. (2020). Computationally guided identification of Akt-3, a serine/threonine kinase inhibitors: Insights from homology modelling, structure-based screening, molecular dynamics and quantum mechanical calculations. Journal of Biomolecular Structure and Dynamics, 38(14), 4179-4188.

Ouimet, B., Pépin, É., Bergeron, Y., Chagniel, L., Beaulieu, J. M., Massicotte, G., & Cyr, M. (2019). Motor learning deficits and striatal GSK-3 hyperactivity in Akt3 knockout mice. Behavioral neuroscience, 133(1), 135.

Fang, Y., Liang, X., Xu, J., & Cai, X. (2018). miR-424 targets AKT3 and PSAT1 and has a tumor-suppressive role in human colorectal cancer. Cancer management and research, 10, 6537.

Ullah, F., Bhattarai, D., Cheng, Z., Liang, X., Deng, T., Rehman, Z. U., ... & Zhang, S. (2018). Comparative analysis of V-AKT murine thymoma viral oncogene homolog 3 (AKT3) gene between cow and buffalo reveals substantial differences for mastitis. BioMed research international, 2018.

Yan, L., Guo, N., Cao, Y., Zeng, S., Wang, J., Lv, F., ... & Cao, X. (2018). miRNA‑145 inhibits myocardial infarction‑induced apoptosis through autophagy via Akt3/mTOR signaling pathway in vitro and in vivo. International journal of molecular medicine, 42(3), 1537-1547.

Howell, K. R., Floyd, K., & Law, A. J. (2017). PKBγ/AKT3 loss-of-function causes learning and memory deficits and deregulation of AKT/mTORC2 signaling: Relevance for schizophrenia. PLoS One, 12(5), e0175993.

Madhunapantula, S. V., & Robertson, G. P. (2017). Targeting protein kinase-b3 (akt3) signaling in melanoma. Expert opinion on therapeutic targets, 21(3), 273-290.

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For research use only. Not intended for any clinical use.

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