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Rabbit Anti-AKT3 Recombinant Antibody (V2-12565) (CBMAB-1055-CN)

This product is a rabbit antibody that recognizes AKT3 of human. The antibody EPR16798 can be used for immunoassay techniques such as: FC, IF, IHC-P, IP, WB.
See all AKT3 antibodies
Published Data
Rabbit Anti-AKT3 Recombinant Antibody (E1Z3W) in IHC
AKT3 is normally detected in excitatory neurons but not PV-positive neurons (yellow arrows) in hippocampal area CA1 and does not show a compensatory increase in expression in Akt1 cKO mice. Scale bar, 40 μm. ...View More
Wong, H., Levenga, J., LaPlante, L., Keller, B., Cooper-Sansone, A., Borski, C., ... & Hoeffer, C. (2020). Isoform-specific roles for AKT in affective behavior, spatial memory, and extinction related to psychiatric disorders. Elife, 9, e56630.
Rabbit Anti-AKT3 Recombinant Antibody (E1Z3W) in WB
PHLDB2 induces EMT by promoting phosphorylation of AKT3. (A) Xiantao website was used to analyze the effect of AKT3 expression on the prognosis of patients with GC. (B) Xiantao was used to analyze the correlation between the expression of AKT3 and EMT markers in GC. (C) The data of TCGA GC were divided into two groups: low-expression group and high-expression group of AKT3. The whole genome expression of these two groups was enriched by GSEA and the enrichment in the EMT pathway was detected by Xiantao. (D) Xiantao was used to analyze the correlation between AKT3 and NR2F1-AS1/miR-190a/PHLDB2 expression in GC. (E,F) The expression of PHLDB2, PI3K, AKT3, and p-AKT was detected by WB after transfection of PHLDB2-OE(EV) and NR2F1-AS1-OE(EV) into BGC823 and SGC7901 cell lines. (G) PHLDB2-OE and EV were transfected into BGC823 and SGC7901 with stable knockout-AKT3 or knockout-control to detect the expression of p-AKT and EMT markers by WB. *p < 0.05; **p < 0.01; ***p < 0.001. ...View More
Lv, J., Zhang, S., Liu, Y., Li, C., Guo, T., Zhang, S., ... & Xu, L. (2021). NR2F1-AS1/MiR-190a/PHLDB2 Induces the Epithelial-Mesenchymal Transformation Process in Gastric Cancer by Promoting Phosphorylation of AKT3. Frontiers in Cell and Developmental Biology, 9, 688949.
Rabbit Anti-AKT3 Recombinant Antibody (E1Z3W) in Immunoblot
Immunoblot analysis of the PARP9, phosphorylated p65 (p-p65), p65, phosphorylated IRF3 at S396 (p-IRF3 S396), phosphorylated IRF3 at S385 (p-IRF3 S385), total IRF3, phosphorylated IRF7 at S477 (p-IRF7 S477), total IRF7, phosphorylated AKT at S473 (p-AKT S473), total AKT3, and β-actin in wild-type (WT) and PARP9 KO BMDC before (0) or various times (above lanes) after VSV infection at MOI of 1. ...View More
Xing, J., Zhang, A., Du, Y., Fang, M., Minze, L. J., Liu, Y. J., ... & Zhang, Z. (2021). Identification of poly (ADP-ribose) polymerase 9 (PARP9) as a noncanonical sensor for RNA virus in dendritic cells. Nature Communications, 12(1), 2681.
Rabbit Anti-AKT3 Recombinant Antibody (E1Z3W) in WB
Western blotting analysis of expression of AKT3, CCND2, CCND1, p21, p-pRb and pRb in GBM cells. β-actin served as the loading control. *P<0.05, vs. miR-610+Vector. ...View More
Mo, X., Cao, Q., Liang, H., Liu, J., Li, H., & Liu, F. (2016). MicroRNA-610 suppresses the proliferation of human glioblastoma cells by repressing CCND2 and AKT3. Molecular medicine reports, 13(3), 1961-1966.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
V2-12565
Antibody Isotype
IgG
Application
IF, IP, WB

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding His140 of human Akt3 protein.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50
IF(ICC)1:800

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 150 mM NaCl, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AKT Serine/Threonine Kinase 3
Introduction
The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
Entrez Gene ID
Human10000
Rat29414
Mouse23797
UniProt ID
HumanQ9Y243
RatQ63484
MouseQ9WUA6
Alternative Names
MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
Function
AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
Biological Process
Brain morphogenesis Source: Ensembl
Homeostasis of number of cells within a tissue Source: Ensembl
Intracellular signal transduction Source: GO_Central
Mitochondrial genome maintenance Source: UniProtKB
Negative regulation of cellular senescence Source: BHF-UCL
Peptidyl-serine phosphorylation Source: GO_Central
Positive regulation of angiogenesis Source: Ensembl
Positive regulation of artery morphogenesis Source: Ensembl
Positive regulation of blood vessel endothelial cell migration Source: BHF-UCL
Positive regulation of cell migration involved in sprouting angiogenesis Source: BHF-UCL
Positive regulation of cell size Source: Ensembl
Positive regulation of endothelial cell proliferation Source: BHF-UCL
Positive regulation of TOR signaling Source: Ensembl
Positive regulation of vascular endothelial cell proliferation Source: BHF-UCL
Protein phosphorylation Source: ProtInc
Signal transduction Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus; Membrane. Membrane-associated after cell stimulation leading to its translocation.
Involvement in disease
AKT3 is a key modulator of several tumors like melanoma, glioma and ovarian cancer. Active AKT3 increases progressively during melanoma tumor progression with highest levels present in advanced-stage metastatic melanomas. Promotes melanoma tumorigenesis by decreasing apoptosis. Plays a key role in the genesis of ovarian cancers through modulation of G2/M phase transition. With AKT2, plays a pivotal role in the biology of glioblastoma.
Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 2 (MPPH2): A syndrome characterized by megalencephaly, hydrocephalus, and polymicrogyria; polydactyly may also be seen. There is considerable phenotypic similarity between this disorder and the megalencephaly-capillary malformation syndrome.
PTM
Phosphorylation on Thr-305 and Ser-472 is required for full activity.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
O-GlcNAcylation at Thr-302 and Thr-309 inhibits activating phosphorylation at Thr-305 via disrupting the interaction between AKT and PDK1.

Shi, F., Yang, Q., Shen, D., & Chen, J. (2021). CircRNA WHSC1 promotes non‐small cell lung cancer progression via sponging microRNA‐296‐3p and up‐regulating expression of AKT serine/threonine kinase 3. Journal of clinical laboratory analysis, e23865.

Hinz, N., Baranowsky, A., Horn, M., Kriegs, M., Sibbertsen, F., Smit, D. J., ... & Jücker, M. (2021). Knockdown of AKT3 Activates HER2 and DDR Kinases in Bone-Seeking Breast Cancer Cells, Promotes Metastasis In Vivo and Attenuates the TGFβ/CTGF Axis. Cells, 10(2), 430.

Srivastava, S., Mehta, P., Sharma, O., Sharma, M., & Malik, R. (2020). Computationally guided identification of Akt-3, a serine/threonine kinase inhibitors: Insights from homology modelling, structure-based screening, molecular dynamics and quantum mechanical calculations. Journal of Biomolecular Structure and Dynamics, 38(14), 4179-4188.

Ouimet, B., Pépin, É., Bergeron, Y., Chagniel, L., Beaulieu, J. M., Massicotte, G., & Cyr, M. (2019). Motor learning deficits and striatal GSK-3 hyperactivity in Akt3 knockout mice. Behavioral neuroscience, 133(1), 135.

Fang, Y., Liang, X., Xu, J., & Cai, X. (2018). miR-424 targets AKT3 and PSAT1 and has a tumor-suppressive role in human colorectal cancer. Cancer management and research, 10, 6537.

Ullah, F., Bhattarai, D., Cheng, Z., Liang, X., Deng, T., Rehman, Z. U., ... & Zhang, S. (2018). Comparative analysis of V-AKT murine thymoma viral oncogene homolog 3 (AKT3) gene between cow and buffalo reveals substantial differences for mastitis. BioMed research international, 2018.

Yan, L., Guo, N., Cao, Y., Zeng, S., Wang, J., Lv, F., ... & Cao, X. (2018). miRNA‑145 inhibits myocardial infarction‑induced apoptosis through autophagy via Akt3/mTOR signaling pathway in vitro and in vivo. International journal of molecular medicine, 42(3), 1537-1547.

Howell, K. R., Floyd, K., & Law, A. J. (2017). PKBγ/AKT3 loss-of-function causes learning and memory deficits and deregulation of AKT/mTORC2 signaling: Relevance for schizophrenia. PLoS One, 12(5), e0175993.

Madhunapantula, S. V., & Robertson, G. P. (2017). Targeting protein kinase-b3 (akt3) signaling in melanoma. Expert opinion on therapeutic targets, 21(3), 273-290.

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For research use only. Not intended for any clinical use.

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