Mouse Anti-AKT3 Recombinant Antibody (V2-180588) (CBMAB-A2122-YC)

Name: Mouse Anti-AKT3 Recombinant Antibody (V2-180588)
SKU: CBMAB-A2122-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AKT3 Recombinant Antibody (CBYC-A506) in WB

Western blot analysis of the different signaling downstream of THAP7-AS1 in both GC cells expressing CUL4B or control cells. ...View More

Liu, H. T., Zou, Y. X., Zhu, W. J., Zhang, G. H., Ma, R. R., Guo, X. Y., & Gao, P. (2022). lncRNA THAP7-AS1, transcriptionally activated by SP1 and post-transcriptionally stabilized by METTL3-mediated m6A modification, exerts oncogenic properties by improving CUL4B entry into the nucleus. Cell Death & Differentiation, 29(3), 627-641.

Mouse Anti-AKT3 Recombinant Antibody (CBYC-A506) in Immunoblot

(A) Glucose increases pAKT levels in ESCC (KYSE70) in a dose-dependent manner. Immunoblot of pAKT/AKT levels in KYSE70 exposed to 0-250 μmol/L glucose in the absence of serum (FBS). (B) Glucose does not alter pAKT levels in EAC (FLO1). Immunoblot of pAKT/AKT levels in FLO1 exposed to 0-250 μmol/L glucose in the absence of serum (FBS). ...View More

Zhang, J., Gelman, I. H., Katsuta, E., Liang, Y., Wang, X., Li, J., ... & Hochwald, S. N. (2019). Glucose Drives Growth Factor-Independent Esophageal Cancer Proliferation via Phosphohistidine-Focal Adhesion Kinase Signaling. Cellular and Molecular Gastroenterology and Hepatology, 8(1), 37-60.

Mouse Anti-AKT3 Recombinant Antibody (CBYC-A506) in Immunoblot

Akt inactivation induced changes in iron metabolism in C2C12 cells. Representative immunoblot for (A) Akt1, Akt2, and Akt3 and (B) FOXO3a; densitometric scanning data after correction for actin loading control are on top of Akt and FOXO3a immunoreactive bands. (C) Ferritin L and (D) ferritin H of stable cell line C2C12 expressing pcDNA3.1 transfected with siRNA against Akt1, Akt2, and Akt3, respectively. Changes in Akt1, Akt2, Akt3 and ferritin L and H densitometry levels were normalized against β-actin. Experiments were performed at least three independent times, yielding comparable results. Bar graphs on the right panel of the figure present a change in protein levels as the mean ± SEM of eight independent experiments, relative to control. ...View More

Halon-Golabek, M., Borkowska, A., Kaczor, J. J., Ziolkowski, W., Flis, D. J., Knap, N., ... & Antosiewicz, J. (2018). hmSOD1 gene mutation-induced disturbance in iron metabolism is mediated by impairment of Akt signalling pathway. Journal of Cachexia, Sarcopenia and Muscle, 9(3), 557-569.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-180588

Application

WB, IP, ELISA

Immunogen

Recombinant Akt3 protein of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

AKT Serine/Threonine Kinase 3

Introduction

AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including

Entrez Gene ID

10000

UniProt ID

Q9Y243

Alternative Names

AKT Serine/Threonine Kinase 3; RAC-PK-Gamma; EC 2.7.11.1; PKB Gamma; STK-2; PKBG; V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma); V-Akt Murine Thymoma Viral Oncogene Homolog 3; RAC-Gamma Serine/Threonine Protein Kinase; RAC-Gamma

Function

AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.

Biological Process

Brain morphogenesis Source: Ensembl
Homeostasis of number of cells within a tissue Source: Ensembl
Intracellular signal transduction Source: GO_Central
Mitochondrial genome maintenance Source: UniProtKB
Negative regulation of cellular senescence Source: BHF-UCL
Peptidyl-serine phosphorylation Source: GO_Central
Positive regulation of angiogenesis Source: Ensembl
Positive regulation of artery morphogenesis Source: Ensembl
Positive regulation of blood vessel endothelial cell migration Source: BHF-UCL
Positive regulation of cell migration involved in sprouting angiogenesis Source: BHF-UCL
Positive regulation of cell size Source: Ensembl
Positive regulation of endothelial cell proliferation Source: BHF-UCL
Positive regulation of TOR signaling Source: Ensembl
Positive regulation of vascular endothelial cell proliferation Source: BHF-UCL
Protein phosphorylation Source: ProtInc
Signal transduction Source: UniProtKB

Cellular Location

Cytoplasm; Nucleus; Membrane. Membrane-associated after cell stimulation leading to its translocation.

Involvement in disease

AKT3 is a key modulator of several tumors like melanoma, glioma and ovarian cancer. Active AKT3 increases progressively during melanoma tumor progression with highest levels present in advanced-stage metastatic melanomas. Promotes melanoma tumorigenesis by decreasing apoptosis. Plays a key role in the genesis of ovarian cancers through modulation of G2/M phase transition. With AKT2, plays a pivotal role in the biology of glioblastoma.
Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 2 (MPPH2): A syndrome characterized by megalencephaly, hydrocephalus, and polymicrogyria; polydactyly may also be seen. There is considerable phenotypic similarity between this disorder and the megalencephaly-capillary malformation syndrome.

PTM

Phosphorylation on Thr-305 and Ser-472 is required for full activity.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
O-GlcNAcylation at Thr-302 and Thr-309 inhibits activating phosphorylation at Thr-305 via disrupting the interaction between AKT and PDK1.

Shi, F., Yang, Q., Shen, D., & Chen, J. (2021). CircRNA WHSC1 promotes non‐small cell lung cancer progression via sponging microRNA‐296‐3p and up‐regulating expression of AKT serine/threonine kinase 3. Journal of clinical laboratory analysis, e23865.

Hinz, N., Baranowsky, A., Horn, M., Kriegs, M., Sibbertsen, F., Smit, D. J., ... & Jücker, M. (2021). Knockdown of AKT3 Activates HER2 and DDR Kinases in Bone-Seeking Breast Cancer Cells, Promotes Metastasis In Vivo and Attenuates the TGFβ/CTGF Axis. Cells, 10(2), 430.

Srivastava, S., Mehta, P., Sharma, O., Sharma, M., & Malik, R. (2020). Computationally guided identification of Akt-3, a serine/threonine kinase inhibitors: Insights from homology modelling, structure-based screening, molecular dynamics and quantum mechanical calculations. Journal of Biomolecular Structure and Dynamics, 38(14), 4179-4188.

Ouimet, B., Pépin, É., Bergeron, Y., Chagniel, L., Beaulieu, J. M., Massicotte, G., & Cyr, M. (2019). Motor learning deficits and striatal GSK-3 hyperactivity in Akt3 knockout mice. Behavioral neuroscience, 133(1), 135.

Fang, Y., Liang, X., Xu, J., & Cai, X. (2018). miR-424 targets AKT3 and PSAT1 and has a tumor-suppressive role in human colorectal cancer. Cancer management and research, 10, 6537.

Ullah, F., Bhattarai, D., Cheng, Z., Liang, X., Deng, T., Rehman, Z. U., ... & Zhang, S. (2018). Comparative analysis of V-AKT murine thymoma viral oncogene homolog 3 (AKT3) gene between cow and buffalo reveals substantial differences for mastitis. BioMed research international, 2018.

Yan, L., Guo, N., Cao, Y., Zeng, S., Wang, J., Lv, F., ... & Cao, X. (2018). miRNA‑145 inhibits myocardial infarction‑induced apoptosis through autophagy via Akt3/mTOR signaling pathway in vitro and in vivo. International journal of molecular medicine, 42(3), 1537-1547.

Howell, K. R., Floyd, K., & Law, A. J. (2017). PKBγ/AKT3 loss-of-function causes learning and memory deficits and deregulation of AKT/mTORC2 signaling: Relevance for schizophrenia. PLoS One, 12(5), e0175993.

Madhunapantula, S. V., & Robertson, G. P. (2017). Targeting protein kinase-b3 (akt3) signaling in melanoma. Expert opinion on therapeutic targets, 21(3), 273-290.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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