Mouse Anti-FUT4 Recombinant Antibody (10) (CBMAB-Z0149-LY)

Name: Mouse Anti-FUT4 Recombinant Antibody (10)
SKU: CBMAB-Z0149-LY
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Published Data

Mouse Anti-FUT4 Recombinant Antibody (10) (CBMAB-Z0149-LY) in WB

Fucosidase activity on defined O-glycoprotein conjugated Lewis epitopes. a-d, Fucosidase activity is observed as a
decrease/loss of Western blot signal on defined conjugated Lewis epitopes presented by the recombinant glyco-engineered P-selectin
glycoprotein ligand-1 (PSGL1) from engineered CHO cells. Each enzyme (2 µM) wasincubated with beads carrying PSGL-1 glycoprotein (displaying
a specific Le antigens) in 20 mM HEPES buffer 150 mM NaCl pH 6.8 at 37 °C for 3 h in 50 µl. The beads were boiled in presence of SDS-loading
buffer containing 25 mM DTT for 10 min at 95°C and western blot analysis was performed (see materials and methods). For simplicity, only the
defined Lewis epitopes of the native protein O-glycome are shown. The enzymatic activity is monitored by the loss of Western blot signal
originating from specific Le-epitope antibodies after enzyme incubation. The cleavage of a fucosyl would be observed as a loss/decrease of signal
due to the large decrease/abolished binding of the antibody. Inactive enzymes serve also as negative controls as they show independently the
full-signal, whereas the active enzymes serve as positive controls, showing signal level when the motif is depleted. The data are from a single
(n=1) experiment. Source data are provided as a Source Data file labelled with the corresponding figure number and panel definition. ...View More

Shuoker, B., Pichler, M. J., Jin, C., Sakanaka, H., Wu, H., Gascueña, A. M., Liu, J., Nielsen, T. S., Holgersson, J., Nordberg Karlsson, E., Juge, N., Meier, S., Morth, J. P., Karlsson, N. G., & Abou Hachem, M. (2023). Sialidases and fucosidases of Akkermansia muciniphila are crucial for growth on mucin and nutrient sharing with mucus-associated gut bacteria. Nature communications, 14(1), 1833.

Mouse Anti-FUT4 Recombinant Antibody (10) (CBMAB-Z0149-LY) in IF

Representative confocal images of secondary tumors from Ptch1+/−/Tis21KO MB allograft treated with MEN1611 or vehicle, labeled with cleaved Caspase-3 (red) and CD15 (green) markers, and the nuclei dye Hoechst (blue). The apoptotic tumor stem cells (CD15+Caspase3+) are indicated by white arrows. Scale bar, 10 μm. ...View More

Ceccarelli, M., D'Andrea, G., Micheli, L., Gentile, G., Cavallaro, S., Merlino, G., Papoff, G., & Tirone, F. (2021). Tumor Growth in the High Frequency Medulloblastoma Mouse Model Ptch1+/-/Tis21KO Has a Specific Activation Signature of the PI3K/AKT/mTOR Pathway and Is Counteracted by the PI3K Inhibitor MEN1611. Frontiers in oncology, 11, 692053.

Mouse Anti-FUT4 Recombinant Antibody (10) (CBMAB-Z0149-LY) in IF

Expression of cell markers in pituispheres. Representative immunofluorescence images of (A) PA02, (B) PA03, and (C) PA05 pituispheres. Cells were double-stained for (A,B) CD15 (green) and GFRα2 (red), (C) Pit-1 (green), and SF-1 (red) (B) CD15 (green) and SF-1 (red) and PA02 pituispheres were single stained for (A) Pit-1 (green) and SF-1 (red) and PA05 pituispheres were single stained for (C) Pit-1 (green) and GFRα2 (red). Isotype-matched antibodies were used for negative controls. Cell nuclei were counterstained with DAPI (blue). Scale bar, 13 μm. ...View More

Peculis, R., Mandrika, I., Petrovska, R., Dortane, R., Megnis, K., Nazarovs, J., Balcere, I., Stukens, J., Konrade, I., Pirags, V., Klovins, J., & Rovite, V. (2020). Pituispheres Contain Genetic Variants Characteristic to Pituitary Adenoma Tumor Tissue. Frontiers in endocrinology, 11, 313.

Mouse Anti-FUT4 Recombinant Antibody (10) (CBMAB-Z0149-LY) in IF

Representative images by confocal microscopy showing the tumor stem cells (CD15+, in red) in MBs from Ptch1+/−/Btg1WT and Ptch1+/−/Btg1KO mice. Nuclei were stained with Hoechst 33258. Scale bar, 50 μm. The white box area is shown with 2.2× magnification. ...View More

Ceccarelli, M., D'Andrea, G., Micheli, L., & Tirone, F. (2020). Deletion of Btg1 Induces Prmt1-Dependent Apoptosis and Increased Stemness in Shh-Type Medulloblastoma Cells Without Affecting Tumor Frequency. Frontiers in oncology, 10, 226.

Mouse Anti-FUT4 Recombinant Antibody (10) (CBMAB-Z0149-LY) in IF

Double-immunofluorescence labeling of human glioma tissue.

Robust anti-CD15 (red) and anti-AQP9 co-labeling was observed in myelomonocytic cells (asterisk). In 4 patient-biopsies weaker CD15 immunolabeling was also observed in a number of larger cells, likely identifying these cells as BTSCs (examples marked by arrowheads). Non-myelomonocytic AQP9 positive cells (examples marked by arrows) were located near putative BTSCs, but no clear examples of AQP9 and CD15 co-expression were observed in these cells. Two areas are shown as overlay images (left), green channel (center) and red channel (right). Nuclei (blue). Scale bar = 20 µm. ...View More

Jelen, S., Parm Ulhøi, B., Larsen, A., Frøkiær, J., Nielsen, S., & Rützler, M. (2013). AQP9 expression in glioblastoma multiforme tumors is limited to a small population of astrocytic cells and CD15(+)/CalB(+) leukocytes. PloS one, 8(9), e75764.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

WB, IP, IF, IHC-P, FC

Immunogen

Purified human neutrophils.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgM, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Fucosyltransferase 4

Introduction

The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15).

Entrez Gene ID

2526

UniProt ID

P22083

Alternative Names

LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV

Function

May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.

Biological Process

Carbohydrate metabolic process Source: ProtInc
Fucosylation Source: GO_Central
L-fucose catabolic process Source: UniProtKB
Oligosaccharide biosynthetic process Source: Reactome
Oligosaccharide metabolic process Source: UniProtKB
Positive regulation of leukocyte tethering or rolling Source: UniProtKB
Protein glycosylation Source: UniProtKB
Regulation of leukocyte cell-cell adhesion Source: UniProtKB

Cellular Location

Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.

Topology

Cytoplasmic: 1-147
Helical: 148-172
Lumenal: 173-530

Aziz, F., Khan, I., Shukla, S., Dey, D. K., Yan, Q., Chakraborty, A., ... & Han, Y. K. (2022). Partners in crime: The Lewis Y antigen and fucosyltransferase IV in Helicobacter pylori-induced gastric cancer. Pharmacology & therapeutics, 232, 107994.

Lu, H. H., Lin, S. Y., Weng, R. R., Juan, Y. H., Chen, Y. W., Hou, H. H., ... & Tsai, H. C. (2020). Fucosyltransferase 4 shapes oncogenic glycoproteome to drive metastasis of lung adenocarcinoma. EBioMedicine, 57, 102846.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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