Human ACACB ELISA Kit (V2LY-0626-LY3662)

Name: Human ACACB ELISA Kit
SKU: V2LY-0626-LY3662
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.023 ng/mL

Detection Range

0.05-30 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

276.5 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Acetyl-CoA Carboxylase Beta

Function

Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism. Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation (By similarity). Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage (By similarity).

Biological Process

Acetyl-CoA metabolic process
Carnitine shuttle
Energy homeostasis
Fatty acid biosynthetic process
Malonyl-CoA biosynthetic process
Negative regulation of catalytic activity
Negative regulation of fatty acid beta-oxidation
Negative regulation of gene expression
Positive regulation of cellular metabolic process
Positive regulation of heart growth
Positive regulation of lipid storage
Protein homotetramerization
Regulation of glucose metabolic process
Regulation of lipid metabolic process
Response to drug
Response to nutrient levels
Response to organic cyclic compound

Cellular Location

Mitochondrion

PTM

The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.
Phosphorylation at Ser-222 by AMPK inactivates the enzyme. Required for the maintenance of skeletal muscle lipid and glucose homeostasis (By similarity).

Sushma, G., Laxmi, P. J., Rao, S. V., Prasad, R. M. V., Kanakachari, M., Prasad, C. S., ... & Bhattacharya, T. K. (2021). In vitro Silencing of Acetyl-CoA Carboxylase beta (ACACB) Gene Reduces Cholesterol Synthesis in Knockdown Chicken Myoblast Cells.

Valvo, V., Iesato, A., Kavanagh, T. R., Priolo, C., Zsengeller, Z., Pontecorvi, A., ... & Nucera, C. (2021). Fine-Tuning Lipid Metabolism by Targeting Mitochondria-Associated Acetyl-CoA-Carboxylase 2 in BRAFV600E Papillary Thyroid Carcinoma. Thyroid.

Catlin, N. R., Bowman, C. J., Campion, S. N., Davenport, S. D., Esler, W. P., Kumpf, S. W., ... & Cappon, G. D. (2021). Inhibition of Acetyl-CoA Carboxylase Causes Malformations in Rats and Rabbits: Comparison of Mammalian Findings and Alternative Assays. Toxicological Sciences, 179(2), 183-194.

Campbell, T. (2020). ACACB encoding mitochondrial enzyme for carboxylation of acetyl-CoA is a novel disease-causing gene for congenital hyperinsulinemia (Doctoral dissertation, University of Cincinnati).

Bhattacharjee, K., Nath, M., & Choudhury, Y. (2020). Fatty acid synthesis and cancer: Aberrant expression of the ACACA and ACACB genes increases the risk for cancer. Meta Gene, 100798.

Tanaka, Y., Kume, S., Maeda, S., Osawa, N., Takeda, N., Chin-Kanasaki, M., ... & Araki, S. I. (2018). Overexpression of acetyl CoA carboxylase β exacerbates podocyte injury in the kidney of streptozotocin-induced diabetic mice. Biochemical and biophysical research communications, 495(1), 1115-1121.

Han, B., Liang, W., Liu, L., Li, Y., & Sun, D. (2018). Genetic association of the ACACB gene with milk yield and composition traits in dairy cattle. Animal genetics, 49(3), 169-177.

Zain, M., Awan, F. R., Najam, S. S., Islam, M., Khan, A. R., Bilal, A., ... & Fumeron, F. (2017). Association of ACACB gene polymorphism (rs2268388, G> A) with type 2 diabetes and end stage renal disease in Pakistani Punjabi population. Meta Gene, 12, 109-112.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-ACACB Recombinant Antibody (V2-179168) (CAT#: CBMAB-A0425-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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