What is the expected molecular weight of ACACB when detected by this antibody in Western Blotting?

ACACB (Acetyl-CoA Carboxylase Beta) is a large protein. When detected by Western Blotting, the Mouse Anti-ACACB Recombinant Antibody (V2-179168) is expected to recognize a band at approximately 270-280 kDa, which corresponds to the full-length protein. Minor variations might occur due to post-translational modifications or splice variants.

How should I store the Mouse Anti-ACACB Recombinant Antibody (V2-179168) to ensure its long-term stability and activity?

For optimal long-term storage, it is highly recommended to aliquot the antibody and store it at -20°C or -80°C to prevent degradation. For short-term use (e.g., within 1-2 weeks), the antibody can be stored at +4°C. It is crucial to minimize freeze-thaw cycles as much as possible, as repeated cycles can denature the antibody and reduce its binding efficiency.

Mouse Anti-ACACB Recombinant Antibody (V2-179168) (CBMAB-A0425-YC)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179168

Application

ELISA, WB

Immunogen

ACACB (NP_001084, 22-120 aa) partial recombinant protein with GST tag. Immunogen sequence: IWGKMTDSKP ITKSKSEANL IPSQEPFPAS DNSGETPQRN GEGHTLPKTP SQAEPASHKG PKDAGRRRNS LPPSHQKPPR NPLSSSDAAP SPELQANGT

Specificity

Human

Antibody Isotype

IgM, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 22-120

More Infomation

Target

Full Name

Acetyl-CoA Carboxylase Beta

Introduction

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty

Entrez Gene ID

UniProt ID

O00763

Alternative Names

Acetyl-CoA Carboxylase Beta; Acetyl-Coenzyme A Carboxylase Beta; Acetyl-CoA Carboxylase 2; ACC-Beta; ACC2; ACCB; EC 6.4.1.2; HACC275;

Function

Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism. Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation (By similarity). Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage (By similarity).

Biological Process

Acetyl-CoA metabolic process
Carnitine shuttle
Energy homeostasis
Fatty acid biosynthetic process
Malonyl-CoA biosynthetic process
Negative regulation of catalytic activity
Negative regulation of fatty acid beta-oxidation
Negative regulation of gene expression
Positive regulation of cellular metabolic process
Positive regulation of heart growth
Positive regulation of lipid storage
Protein homotetramerization
Regulation of glucose metabolic process
Regulation of lipid metabolic process
Response to drug
Response to nutrient levels
Response to organic cyclic compound

Cellular Location

Mitochondrion

PTM

The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.
Phosphorylation at Ser-222 by AMPK inactivates the enzyme. Required for the maintenance of skeletal muscle lipid and glucose homeostasis (By similarity).

Sushma, G., Laxmi, P. J., Rao, S. V., Prasad, R. M. V., Kanakachari, M., Prasad, C. S., ... & Bhattacharya, T. K. (2021). In vitro Silencing of Acetyl-CoA Carboxylase beta (ACACB) Gene Reduces Cholesterol Synthesis in Knockdown Chicken Myoblast Cells.

Valvo, V., Iesato, A., Kavanagh, T. R., Priolo, C., Zsengeller, Z., Pontecorvi, A., ... & Nucera, C. (2021). Fine-Tuning Lipid Metabolism by Targeting Mitochondria-Associated Acetyl-CoA-Carboxylase 2 in BRAFV600E Papillary Thyroid Carcinoma. Thyroid.

Catlin, N. R., Bowman, C. J., Campion, S. N., Davenport, S. D., Esler, W. P., Kumpf, S. W., ... & Cappon, G. D. (2021). Inhibition of Acetyl-CoA Carboxylase Causes Malformations in Rats and Rabbits: Comparison of Mammalian Findings and Alternative Assays. Toxicological Sciences, 179(2), 183-194.

Campbell, T. (2020). ACACB encoding mitochondrial enzyme for carboxylation of acetyl-CoA is a novel disease-causing gene for congenital hyperinsulinemia (Doctoral dissertation, University of Cincinnati).

Bhattacharjee, K., Nath, M., & Choudhury, Y. (2020). Fatty acid synthesis and cancer: Aberrant expression of the ACACA and ACACB genes increases the risk for cancer. Meta Gene, 100798.

Tanaka, Y., Kume, S., Maeda, S., Osawa, N., Takeda, N., Chin-Kanasaki, M., ... & Araki, S. I. (2018). Overexpression of acetyl CoA carboxylase β exacerbates podocyte injury in the kidney of streptozotocin-induced diabetic mice. Biochemical and biophysical research communications, 495(1), 1115-1121.

Han, B., Liang, W., Liu, L., Li, Y., & Sun, D. (2018). Genetic association of the ACACB gene with milk yield and composition traits in dairy cattle. Animal genetics, 49(3), 169-177.

Zain, M., Awan, F. R., Najam, S. S., Islam, M., Khan, A. R., Bilal, A., ... & Fumeron, F. (2017). Association of ACACB gene polymorphism (rs2268388, G> A) with type 2 diabetes and end stage renal disease in Pakistani Punjabi population. Meta Gene, 12, 109-112.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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