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Mouse Anti-ACACB Recombinant Antibody (V2-179168) (CBMAB-A0425-YC)

Provided herein is a Mouse monoclonal antibody against Human Acetyl-CoA Carboxylase Beta. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all ACACB antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-179168
Antibody Isotype
IgM, κ
Application
ELISA, WB

Basic Information

Immunogen
ACACB (NP_001084, 22-120 aa) partial recombinant protein with GST tag. Immunogen sequence: IWGKMTDSKP ITKSKSEANL IPSQEPFPAS DNSGETPQRN GEGHTLPKTP SQAEPASHKG PKDAGRRRNS LPPSHQKPPR NPLSSSDAAP SPELQANGT
Specificity
Human
Antibody Isotype
IgM, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 22-120

Target

Full Name
Acetyl-CoA Carboxylase Beta
Introduction
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty
Entrez Gene ID
32
UniProt ID
O00763
Alternative Names
Acetyl-CoA Carboxylase Beta; Acetyl-Coenzyme A Carboxylase Beta; Acetyl-CoA Carboxylase 2; ACC-Beta; ACC2; ACCB; EC 6.4.1.2; HACC275;
Function
Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism. Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation (By similarity). Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage (By similarity).
Biological Process
Acetyl-CoA metabolic process
Carnitine shuttle
Energy homeostasis
Fatty acid biosynthetic process
Malonyl-CoA biosynthetic process
Negative regulation of catalytic activity
Negative regulation of fatty acid beta-oxidation
Negative regulation of gene expression
Positive regulation of cellular metabolic process
Positive regulation of heart growth
Positive regulation of lipid storage
Protein homotetramerization
Regulation of glucose metabolic process
Regulation of lipid metabolic process
Response to drug
Response to nutrient levels
Response to organic cyclic compound
Cellular Location
Mitochondrion
PTM
The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.
Phosphorylation at Ser-222 by AMPK inactivates the enzyme. Required for the maintenance of skeletal muscle lipid and glucose homeostasis (By similarity).

Sushma, G., Laxmi, P. J., Rao, S. V., Prasad, R. M. V., Kanakachari, M., Prasad, C. S., ... & Bhattacharya, T. K. (2021). In vitro Silencing of Acetyl-CoA Carboxylase beta (ACACB) Gene Reduces Cholesterol Synthesis in Knockdown Chicken Myoblast Cells.

Valvo, V., Iesato, A., Kavanagh, T. R., Priolo, C., Zsengeller, Z., Pontecorvi, A., ... & Nucera, C. (2021). Fine-Tuning Lipid Metabolism by Targeting Mitochondria-Associated Acetyl-CoA-Carboxylase 2 in BRAFV600E Papillary Thyroid Carcinoma. Thyroid.

Catlin, N. R., Bowman, C. J., Campion, S. N., Davenport, S. D., Esler, W. P., Kumpf, S. W., ... & Cappon, G. D. (2021). Inhibition of Acetyl-CoA Carboxylase Causes Malformations in Rats and Rabbits: Comparison of Mammalian Findings and Alternative Assays. Toxicological Sciences, 179(2), 183-194.

Campbell, T. (2020). ACACB encoding mitochondrial enzyme for carboxylation of acetyl-CoA is a novel disease-causing gene for congenital hyperinsulinemia (Doctoral dissertation, University of Cincinnati).

Bhattacharjee, K., Nath, M., & Choudhury, Y. (2020). Fatty acid synthesis and cancer: Aberrant expression of the ACACA and ACACB genes increases the risk for cancer. Meta Gene, 100798.

Tanaka, Y., Kume, S., Maeda, S., Osawa, N., Takeda, N., Chin-Kanasaki, M., ... & Araki, S. I. (2018). Overexpression of acetyl CoA carboxylase β exacerbates podocyte injury in the kidney of streptozotocin-induced diabetic mice. Biochemical and biophysical research communications, 495(1), 1115-1121.

Han, B., Liang, W., Liu, L., Li, Y., & Sun, D. (2018). Genetic association of the ACACB gene with milk yield and composition traits in dairy cattle. Animal genetics, 49(3), 169-177.

Zain, M., Awan, F. R., Najam, S. S., Islam, M., Khan, A. R., Bilal, A., ... & Fumeron, F. (2017). Association of ACACB gene polymorphism (rs2268388, G> A) with type 2 diabetes and end stage renal disease in Pakistani Punjabi population. Meta Gene, 12, 109-112.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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