Mouse Cd68 ELISA Kit (V2LY-0626-LY2594)

Name: Mouse Cd68 ELISA Kit
SKU: V2LY-0626-LY2594
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.025 ng/mL

Detection Range

0.05-20 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Mouse

Assay Type

Sandwich

Reactivity

Mouse

Assay Time

1.5 h

Molecule Mass

34.8 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

CD68 Molecule

Alternative Names

CD68 Molecule; CD68 Antigen; Scavenger Receptor Class D, Member 1; Macrophage Antigen CD68; GP110; Macrosialin; SCARD1; LAMP4;

Function

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Biological Process

Aging Source: ARUK-UCL
Autocrine signaling Source: Ensembl
Cellular response to lipopolysaccharide Source: ARUK-UCL
Cellular response to nutrient levels Source: ARUK-UCL
Cellular response to oxidised low-density lipoprotein particle stimulus Source: ARUK-UCL
Establishment of protein localization to organelle Source: GO_Central
Inflammatory response to antigenic stimulus Source: ARUK-UCL
Negative regulation of dendritic cell antigen processing and presentation Source: ARUK-UCL
Neutrophil degranulation Source: Reactome

Cellular Location

Isoform Short: Cell membrane
Isoform Long: Lysosome membrane; Endosome membrane

Topology

Extracellular: 22-319
Helical: 320-344
Cytoplasmic: 345-354

PTM

N- and O-glycosylated.

Song, Y. J., Xu, Y., Zhu, X., Fu, J., Deng, C., Chen, H., ... & Wang, J. (2020). Immune landscape of the tumor microenvironment identifies prognostic gene signature CD4/CD68/CSF1R in osteosarcoma. Frontiers in oncology, 10, 1198.

Neri, M., Frati, A., Turillazzi, E., Cantatore, S., Cipolloni, L., Di Paolo, M., ... & Fineschi, V. (2018). Immunohistochemical evaluation of aquaporin-4 and its correlation with CD68, IBA-1, HIF-1α, GFAP, and CD15 expressions in fatal traumatic brain injury. International journal of molecular sciences, 19(11), 3544.

Caliò, A., Brunelli, M., Segala, D., Pedron, S., Tardanico, R., Remo, A., ... & Martignoni, G. (2018). t (6; 11) renal cell carcinoma: a study of seven cases including two with aggressive behavior, and utility of CD68 (PG-M1) in the differential diagnosis with pure epithelioid PEComa/epithelioid angiomyolipoma. Modern Pathology, 31(3), 474-487.

Wang, L., Zhang, C., Zhang, Z., Han, B., Shen, Z., Li, L., ... & Zhang, Y. (2018). Specific clinical and immune features of CD68 in glioma via 1,024 samples. Cancer management and research, 10, 6409.

Chistiakov, D. A., Killingsworth, M. C., Myasoedova, V. A., Orekhov, A. N., & Bobryshev, Y. V. (2017). CD68/macrosialin: not just a histochemical marker. Laboratory Investigation, 97(1), 4-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD68 protein, His Tag (CAT#: V2LY-0526-LY2918)

Human Recombinant CD68 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2919)

Mouse Recombinant CD68 protein, ECD, His Tag (CAT#: V2LY-0526-LY8056)

Rat Recombinant CD68 protein, hFc Tag (CAT#: V2LY-0526-LY9261)

Rat Recombinant CD68 protein, His Tag (CAT#: V2LY-0526-LY9262)

Rat Recombinant CD68 protein, Biotin Conjugated, (CAT#: V2LY-0526-LY9263)

Rat Recombinant CD68 protein (CAT#: V2LY-0526-LY9264)

Related Products

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Mouse Anti-CD68 Recombinant Antibody (Y1/82A) (CAT#: CBMAB-C2094-CQ)

Mouse Anti-CD68 Recombinant Antibody (AG36) (CAT#: V2LY-0524-LY35)

Mouse Anti-CD68 Recombinant Antibody (SPM130) (CAT#: CBMAB-C1066-CQ)

Mouse Anti-CD68 Recombinant Antibody (CBT313) (CAT#: V2LY-0625-LY3608)

Mouse Anti-CD68 Recombinant Antibody (CBT3455) (CAT#: V2LY-0625-LY89)

Rat Recombinant CD68 protein, His Tag (CAT#: V2LY-0526-LY9262)

Rabbit Anti-CD68 Recombinant Antibody (039) (CAT#: CBMAB-C1734-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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