Mouse Hs3st2 ELISA Kit (V2LY-0626-LY5867)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.0093 ng/mL
Detection Range
0.02-4.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
41.4 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
heparan sulfate (glucosamine) 3-O-sulfotransferase 2
Function
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate (PubMed:9988768).

Catalyzes the O-sulfation of glucosamine in GlcA2S-GlcNS (PubMed:9988768).

Unlike HS3ST1/3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate (PubMed:9988768).
Biological Process
Glycosaminoglycan biosynthetic process Source: Reactome
Cellular Location
Golgi apparatus membrane
Topology
Cytoplasmic: 1-19
Helical: 20-39
Lumenal: 40-367

Zhang, F., Li, F., & Lu, J. (2023). microRNA-100 shuttled by human umbilical cord MSC-secreted extracellular vesicles induces endometriosis by inhibiting HS3ST2. Cellular Signalling, 102, 110532.

Huynh, M. B., Rebergue, N., Merrick, H., Gomez-Henao, W., Jospin, E., Biard, D. S. F., & Papy-Garcia, D. (2022). HS3ST2 expression induces the cell autonomous aggregation of tau. Scientific Reports, 12(1), 10850.

Li, L., Gao, Y., Han, Z., Du, J., Yu, Y., Zhang, N., ... & Wang, M. (2022). Modified risk of breast cancer by dietary factors and HS3ST2 methylation.

Gao, G., Shi, X., & Shen, J. (2021). HS3ST2 and its related molecules as potential biomarkers for predicting lymph node metastasis in patients with colorectal cancer. OncoTargets and therapy, 3881-3894.

Teixeira, F. C., Vijaya Kumar, A., Kumar Katakam, S., Cocola, C., Pelucchi, P., Graf, M., ... & Götte, M. (2020). The heparan sulfate sulfotransferases HS2ST1 and HS3ST2 are novel regulators of breast cancer stem-cell properties. Frontiers in Cell and Developmental Biology, 8, 559554.

Eyvazi, S., Khamaneh, A. M., Tarhriz, V., Bandehpour, M., Hejazi, M. S., Sadat, A. T. E., & Sepehri, B. (2020). CpG islands methylation analysis of CDH11, EphA5, and HS3ST2 genes in gastric adenocarcinoma patients. Journal of gastrointestinal cancer, 51, 579-583.

Meng, L., Li, M., Gao, Z., Ren, H., Chen, J., Liu, X., ... & Yang, L. (2019). Possible role of hsa-miR-194-5p, via regulation of HS3ST2, in the pathogenesis of atopic dermatitis in children. European Journal of Dermatology, 29, 603-613.

Delos, M., Foulquier, F., Hellec, C., Vicogne, D., Fifre, A., Carpentier, M., ... & Denys, A. (2018). Heparan sulfate 3-O-sulfotransferase 2 (HS3ST2) displays an unexpected subcellular localization in the plasma membrane. Biochimica et Biophysica Acta (BBA)-General Subjects, 1862(7), 1644-1655.

Danková, Z., Braný, D., Dvorská, D., Ňachajová, M., Fiolka, R., Grendár, M., ... & Žúbor, P. (2018). Methylation status of KLF4 and HS3ST2 genes as predictors of endometrial cancer and hyperplastic endometrial lesions. International journal of molecular medicine, 42(6), 3318-3328.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human HS3ST2 ELISA Kit (CAT#: V2LY-0626-LY4659)

Related Products

Human HS3ST2 ELISA Kit (CAT#: V2LY-0626-LY4659)

Mouse Anti-HS3ST2 Recombinant Antibody (CBFYH-1976) (CAT#: CBMAB-H0727-FY)

Mouse Anti-HS3ST2 Recombinant Antibody (5D5) (CAT#: CBMAB-A4097-LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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