Mouse Mc4r ELISA Kit (V2LY-0626-LY2615)

Name: Mouse Mc4r ELISA Kit
SKU: V2LY-0626-LY2615
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0015 ng/mL

Detection Range

0.003-0.9 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Mouse

Assay Type

Sandwich

Reactivity

Mouse

Assay Time

1.5 h

Molecule Mass

37.0 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

MELANOCORTIN 4 RECEPTOR

Function

Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. Plays a central role in energy homeostasis and somatic growth. This receptor is mediated by G proteins that stimulate adenylate cyclase (cAMP).

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: BHF-UCL
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: ProtInc
Diet induced thermogenesis Source: Ensembl
Energy reserve metabolic process Source: Ensembl
Feeding behavior Source: ProtInc
Insulin secretion Source: Ensembl
Negative regulation of feeding behavior Source: Ensembl
Positive regulation of bone resorption Source: HGNC-UCL
Regulation of eating behavior Source: ARUK-UCL
Regulation of grooming behavior Source: Ensembl
Regulation of metabolic process Source: GO_Central
Response to insulin Source: Ensembl
Response to melanocyte-stimulating hormone Source: ARUK-UCL

Cellular Location

Cell membrane

Involvement in disease

Obesity (OBESITY):
A condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.

Topology

Extracellular: 1-43
Helical: 44-69
Cytoplasmic: 70-81
Helical: 82-106
Extracellular: 107-123
Helical: 124-145
Cytoplasmic: 146-165
Helical: 166-186
Extracellular: 187-191
Helical: 192-215
Cytoplasmic: 216-248
Helical: 249-271
Extracellular: 272-280
Helical: 281-304
Cytoplasmic: 305-332

Seelig, E., Henning, E., Keogh, J. M., Gillett, D., Shin, E., Buscombe, J., ... & Farooqi, I. S. (2022). Obesity due to melanocortin 4 receptor (MC4R) deficiency is associated with delayed gastric emptying. Clinical endocrinology, 96(2), 270-275.

Fatima, M. T., Ahmed, I., Fakhro, K. A., & Akil, A. S. A. S. (2022). Melanocortin‐4 receptor complexity in energy homeostasis, obesity and drug development strategies. Diabetes, Obesity and Metabolism, 24(4), 583-598.

Zhang, H., Chen, L. N., Yang, D., Mao, C., Shen, Q., Feng, W., ... & Zhang, Y. (2021). Structural insights into ligand recognition and activation of the melanocortin-4 receptor. Cell Research, 31(11), 1163-1175.

Zhang, J., Li, J., Wu, C., Hu, Z., An, L., Wan, Y., ... & Wang, Y. (2020). The Asp298Asn polymorphism of melanocortin‐4 receptor (MC4R) in pigs: evidence for its potential effects on MC4R constitutive activity and cell surface expression. Animal Genetics, 51(5), 694-706.

Hainer, V., Hainerová, I. A., Kunešová, M., Braunerová, R. T., Zamrazilova, H., & Bendlová, B. (2020). Melanocortin pathways: suppressed and stimulated melanocortin-4 receptor (MC4R). Physiological Research, 69(Suppl 2), S245.

Kleinau, G., Heyder, N. A., Tao, Y. X., & Scheerer, P. (2020). Structural complexity and plasticity of signaling regulation at the melanocortin-4 receptor. International Journal of Molecular Sciences, 21(16), 5728.

Yu, J., Gimenez, L. E., Hernandez, C. C., Wu, Y., Wein, A. H., Han, G. W., ... & Stevens, R. C. (2020). Determination of the melanocortin-4 receptor structure identifies Ca2+ as a cofactor for ligand binding. Science, 368(6489), 428-433.

Sharma, S., Garfield, A. S., Shah, B., Kleyn, P., Ichetovkin, I., Moeller, I. H., ... & Van der Ploeg, L. H. (2019). Current mechanistic and pharmacodynamic understanding of melanocortin-4 receptor activation. Molecules, 24(10), 1892.

Kühnen, P., Krude, H., & Biebermann, H. (2019). Melanocortin-4 receptor signalling: importance for weight regulation and obesity treatment. Trends in molecular medicine, 25(2), 136-148.

Heyder, N., Kleinau, G., Szczepek, M., Kwiatkowski, D., Speck, D., Soletto, L., ... & Scheerer, P. (2019). Signal transduction and pathogenic modifications at the melanocortin-4 receptor: A structural perspective. Frontiers in endocrinology, 10, 515.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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