Mouse Pou5f1 ELISA Kit (V2LY-0626-LY2887)

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Tested Data
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Basic Information

Sensitivity
0.0095 ng/mL
Detection Range
0.02-6 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
38.2 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
POU class 5 homeobox 1
Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Biological Process
Anatomical structure morphogenesisManual Assertion Based On ExperimentTAS:ProtInc
Blastocyst developmentISS:BHF-UCL
BMP signaling pathway involved in heart inductionIMP:BHF-UCL
Cardiac cell fate determinationIDA:BHF-UCL
Cell fate commitment involved in formation of primary germ layerIMP:BHF-UCL
Endodermal cell fate specificationManual Assertion Based On ExperimentIDA:MGI
Negative regulation of miRNA transcriptionManual Assertion Based On ExperimentIDA:ARUK-UCL
Negative regulation of miRNA-mediated gene silencingManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of canonical Wnt signaling pathwayIDA:BHF-UCL
Positive regulation of SMAD protein signal transductionIDA:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Regulation of asymmetric cell divisionISS:BHF-UCL
Regulation of DNA methylation-dependent heterochromatin assemblyIDA:BHF-UCL
Regulation of gene expressionManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of heart induction by regulation of canonical Wnt signaling pathwayIMP:BHF-UCL
Regulation of transcription by RNA polymerase IIISS:BHF-UCL
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:HGNC-UCL
Response to woundingManual Assertion Based On ExperimentIEP:UniProtKB
Somatic stem cell population maintenanceManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
Nucleus
Expressed in a diffuse and slightly punctuate pattern. Colocalizes with MAPK8 and MAPK9 in the nucleus.
PTM
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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