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POU5F1

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Full Name
POU class 5 homeobox 1
Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Biological Process
Anatomical structure morphogenesisManual Assertion Based On ExperimentTAS:ProtInc
Blastocyst developmentISS:BHF-UCL
BMP signaling pathway involved in heart inductionIMP:BHF-UCL
Cardiac cell fate determinationIDA:BHF-UCL
Cell fate commitment involved in formation of primary germ layerIMP:BHF-UCL
Endodermal cell fate specificationManual Assertion Based On ExperimentIDA:MGI
Negative regulation of miRNA transcriptionManual Assertion Based On ExperimentIDA:ARUK-UCL
Negative regulation of miRNA-mediated gene silencingManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of canonical Wnt signaling pathwayIDA:BHF-UCL
Positive regulation of SMAD protein signal transductionIDA:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Regulation of asymmetric cell divisionISS:BHF-UCL
Regulation of DNA methylation-dependent heterochromatin assemblyIDA:BHF-UCL
Regulation of gene expressionManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of heart induction by regulation of canonical Wnt signaling pathwayIMP:BHF-UCL
Regulation of transcription by RNA polymerase IIISS:BHF-UCL
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:HGNC-UCL
Response to woundingManual Assertion Based On ExperimentIEP:UniProtKB
Somatic stem cell population maintenanceManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
Nucleus
Expressed in a diffuse and slightly punctuate pattern. Colocalizes with MAPK8 and MAPK9 in the nucleus.
PTM
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.

Anti-POU5F1 antibodies

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Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse
Clone: CBXO-0023
Application*: WB, IF
Target: POU5F1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Mouse
Clone: CBXO-0022
Application*: WB, IP, IF, F, CI
Target: POU5F1
Host: Rabbit
Specificity: Human, Mouse
Clone: CBXO-0020
Application*: F
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse
Clone: A3-C11
Application*: WB, IF
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 7E7
Application*: WB, E, F, IF
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Mouse, Human
Clone: 3A5
Application*: WB, E, F, IF
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human, Mouse, Rat
Clone: SEMGC
Application*: P, ICC, IF
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG3
Specificity: Human
Clone: PR-2H9
Application*: E, WB
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: PR-1A6
Application*: WB, DB
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: NRG1.1
Application*: P
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: GT735
Application*: WB, IP, F, ICC, IF
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human, Mouse
Clone: GT486
Application*: WB, IP, P, F, ICC, IF
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG
Specificity: Human
Clone: 4H2
Application*: E, WB, P
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: CBYC3-107
Application*: E, WB
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Mouse
Clone: 2F12
Application*: IP, WB
Target: POU5F1
Host: Mouse
Specificity: Human, Mouse
Clone: CBXO-0021
Application*: WB, IF, F
Target: POU5F1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse
Clone: CBXO-0524
Application*: CI
Target: POU5F1
Host: Rabbit
Specificity: Human
Clone: CBXO-0528
Application*: WB, P, IF, F, CI
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBXO-0173
Application*: F
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse
Clone: CBXO-0293
Application*: F, IC, WB
Target: POU5F1
Host: Mouse
Specificity: Human, Mouse
Clone: CBXO-0246
Application*: WB
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXO-0532
Application*: E, WB
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXO-0531
Application*: WB
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXO-0225
Application*: E, F, WB
Target: POU5F1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse
Clone: CBXO-0525
Application*: F, IC, IF, WB
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXO-0224
Application*: E, WB
Target: POU5F1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CBXO-0135
Application*: F, P, IP, WB
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: CBXO-0530
Application*: WB
Target: POU5F1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CBXO-0447
Application*: CI, F, IC, IF, WB
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXO-0223
Application*: E, WB
Target: POU5F1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse
Clone: CBXO-0500
Application*: CI, F, IC, IF, WB
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG
Specificity: Mouse, Rat, Human
Clone: CBXO-0317
Application*: WB, IP, IF, P, F, E
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Mouse, Rat, Human
Clone: CBXO-0344
Application*: WB, IP, IF, E
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: MRQ-10
Application*: P, C
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: CBXO-0529
Application*: WB, IF, IS
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: CBXO-0533
Application*: P
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse
Clone: CBXO-0445
Application*: WB, IS, IF
Target: POU5F1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse
Clone: C30A3
Application*: WB, IF, F
Target: POU5F1
Host: Human
Antibody Isotype: IgG1
Specificity: Human, Mouse
Clone: CBXO-0433
Application*: F
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBXO-0390
Application*: WB, IS, IC, MC
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Mouse, Human
Clone: CBXO-0527
Application*: WB, IF
Target: POU5F1
Host: Rat
Antibody Isotype: IgG2b
Specificity: Human, Mouse
Clone: CBXO-0372
Application*: WB, IS, IC, MC
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Mouse
Clone: 9B7
Application*: F, IC, IF, WB
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse
Clone: CF347
Application*: ELISA, WB, IHC
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CB420A
Application*: ELISA, WB, ICC, FC
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human, Mouse, Rat
Clone: CB24A
Application*: ELISA, WB, IHC, IF, FC
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: IHC643
Application*: P, E, IH
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse
Clone: D7O5Z
Application*: WB, IF (IC), F
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: 4F8
Application*: WB, E
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human, Mouse, Rat
Clone: 2H41D8
Application*: E, WB, IH, IF, F
Target: POU5F1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CBCNC-687
Application*: E
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse
Clone: 9H260
Application*: WB, ICC, FC
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 5F7
Application*: WB, E
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: CBYC-P524
Application*: F, IC, WB
Target: POU5F1
Host: Mouse
Antibody Isotype: IgM
Specificity: Human, Mouse
Clone: 341CT7.6.2
Application*: WB, E
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: 1B11
Application*: E, IF, WB
Target: POU5F1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 1A4
Application*: IP, WB, M
Target: POU5F1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse
Clone: C30A3C1
Application*: CI
Target: POU5F1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: C52G3
Application*: WB, P, IF (IC), F, CI
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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