Mouse Recombinant CA9 protein, Biotin Conjugated, His Tag (V2LY-0526-LY7907)

Name: Mouse Recombinant CA9 protein, Biotin Conjugated, His Tag
SKU: V2LY-0526-LY7907
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant CA9 protein, Biotin Conjugated, His Tag consist of Amino Acid: 1-390 and predicts a molecular mass of 40.3 kDa.

Molecule Mass

40.3 kDa

Conjugates

Biotin

Sequence

Amino Acid: 1-390

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

carbonic anhydrase 9

Function

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

Biological Process

Bicarbonate transport Source: Reactome
Morphogenesis of an epithelium Source: Ensembl
One-carbon metabolic process Source: GO_Central
Regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: Reactome
Response to drug Source: Ensembl
Response to testosterone Source: Ensembl
Secretion Source: Ensembl

Cellular Location

Nucleus; Nucleolus; Cell membrane; Microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.

Topology

Extracellular: 38-414 aa
Helical: 415-435 aa
Cytoplasmic: 436-459 aa

PTM

Asn-346 bears high-mannose type glycan structures.

Giatromanolaki, A., Harris, A. L., Banham, A. H., Contrafouris, C. A., & Koukourakis, M. I. (2020). Carbonic anhydrase 9 (CA9) expression in non-small-cell lung cancer: correlation with regulatory FOXP3+ T-cell tumour stroma infiltration. British journal of cancer, 122(8), 1205-1210.

Baniak, N., Flood, T. A., Buchanan, M., Dal Cin, P., & Hirsch, M. S. (2020). Carbonic anhydrase IX (CA9) expression in multiple renal epithelial tumour subtypes. Histopathology, 77(4), 659-666.

Wang, S., Fu, Z., Wang, Y., Sun, Y., Cui, L., Wang, C., ... & Wen, N. (2020). Correlation of carbonic anhydrase 9 (CA9) with pathological T-stage and prognosis in patients with oral tongue squamous cell carcinoma. Annals of Translational Medicine, 8(22).

Li, Z., Jiang, L., Chew, S. H., Hirayama, T., Sekido, Y., & Toyokuni, S. (2019). Carbonic anhydrase 9 confers resistance to ferroptosis/apoptosis in malignant mesothelioma under hypoxia. Redox biology, 26, 101297.

Eckert, A. W., Horter, S., Bethmann, D., Kotrba, J., Kaune, T., Rot, S., ... & Kappler, M. (2019). Investigation of the prognostic role of carbonic anhydrase 9 (CAIX) of the cellular mRNA/protein level or soluble CAIX protein in patients with oral squamous cell carcinoma. International journal of molecular sciences, 20(2), 375.

McDonald, P. C., Chafe, S. C., Brown, W. S., Saberi, S., Swayampakula, M., Venkateswaran, G., ... & Dedhar, S. (2019). Regulation of pH by carbonic anhydrase 9 mediates survival of pancreatic cancer cells with activated KRAS in response to hypoxia. Gastroenterology, 157(3), 823-837.

Finkelmeier, F., Canli, Ö., Peiffer, K. H., Walter, D., Tal, A., Koch, C., ... & Waidmann, O. (2018). Circulating hypoxia marker carbonic anhydrase IX (CA9) in patients with hepatocellular carcinoma and patients with cirrhosis. PloS one, 13(7), e0200855.

Kim, J. Y., Lee, S. H., An, S., Kim, S. J., Sung, Y. N., Song, K. B., ... & Hong, S. M. (2018). Carbonic anhydrase 9 expression in well-differentiated pancreatic neuroendocrine neoplasms might be associated with aggressive behavior and poor survival. Virchows Archiv, 472(5), 739-748.

Horie, K., Kawakami, K., Fujita, Y., Sugaya, M., Kameyama, K., Mizutani, K., ... & Ito, M. (2017). Exosomes expressing carbonic anhydrase 9 promote angiogenesis. Biochemical and biophysical research communications, 492(3), 356-361.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Canine Recombinant CA9 protein, hFc Tag (CAT#: V2LY-0526-LY1213)

Canine Recombinant CA9 protein, His Tag (CAT#: V2LY-0526-LY1214)

Human Recombinant CA9 protein, ECD, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2554)

Human Recombinant CA9 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2555)

Human Recombinant CA9 protein, ECD, His Tag (CAT#: V2LY-0526-LY2556)

Human Recombinant CA9 protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY2557)

Human Recombinant CA9 protein, hFc Tag (CAT#: V2LY-0526-LY2558)

Mouse Recombinant CA9 protein, His Tag (CAT#: V2LY-0526-LY7908)

CA9 Matched Antibody Pair (165) (CAT#: APMAB-165LY)

Antibody Pairs

CA9 Matched Antibody Pair (165) (CAT#: APMAB-165LY)

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Mouse Recombinant CA9 protein, His Tag (CAT#: V2LY-0526-LY7908)

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Canine Recombinant CA9 protein, hFc Tag (CAT#: V2LY-0526-LY1213)

CA9 Matched Antibody Pair (165) (CAT#: APMAB-165LY)

Mouse Anti-CA9 Recombinant Antibody (326) (CAT#: CBMAB-AP722LY)

Mouse Anti-CA9 Recombinant Antibody (CBT4348) (CAT#: V2LY-0625-LY63)

Human Recombinant CA9 protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY2557)

Mouse Anti-CA9 Recombinant Antibody (CBT4395) (CAT#: V2LY-0625-LY62)

Rabbit Anti-CA9 Recombinant Antibody (036) (CAT#: CBMAB-C1724-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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