Mouse Recombinant CD47 protein, Biotin Conjugated, His & AVI Tag (V2LY-0526-LY8033)

Name: Mouse Recombinant CD47 protein, Biotin Conjugated, His & AVI Tag
SKU: V2LY-0526-LY8033
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His & AVI Tag

Protein Construction

This product is Mouse Recombinant CD47 protein, Biotin Conjugated, His & AVI Tag consist of Amino Acid: 1-140 and predicts a molecular mass of 17.1 kDa.

Molecule Mass

17.1 kDa

Verified

HPLC

Conjugates

Biotin

Sequence

Amino Acid: 1-140

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD47 Molecule

Alternative Names

CD47 Molecule; Antigenic Surface Determinant Protein OA3; CD47 Antigen (Rh-Related Antigen, Integrin-Associated Signal Transducer); Antigen Identified By Monoclonal Antibody 1D8; Leukocyte Surface Antigen CD47; Integrin Associated Protein; Rh-Related Antigen; CD47 Glycoprotein;

Function

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity).
Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Biological Process

ATP export Source: ARUK-UCL
Cell migration Source: ARUK-UCL
Cellular response to interferon-gamma Source: ARUK-UCL
Cellular response to interleukin-1 Source: ARUK-UCL
Cellular response to interleukin-12 Source: ARUK-UCL
Extracellular matrix organization Source: Reactome
Integrin-mediated signaling pathway Source: ProtInc
Leukocyte migration Source: Reactome
Monocyte extravasation Source: ARUK-UCL
Negative regulation of Fc-gamma receptor signaling pathway involved in phagocytosis Source: ARUK-UCL
Neutrophil degranulation Source: Reactome
Positive regulation of cell-cell adhesion Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of inflammatory response Source: GO_Central
Positive regulation of phagocytosis Source: GO_Central
Positive regulation of stress fiber assembly Source: ARUK-UCL
Positive regulation of T cell activation Source: UniProtKB
Regulation of interferon-gamma production Source: ARUK-UCL
Regulation of interleukin-10 production Source: ARUK-UCL
Regulation of interleukin-12 production Source: ARUK-UCL
Regulation of interleukin-6 production Source: ARUK-UCL
Regulation of nitric oxide biosynthetic process Source: ARUK-UCL
Regulation of tumor necrosis factor production Source: ARUK-UCL

Cellular Location

Cell membrane

Topology

Extracellular: 19-141
Helical: 142-162
Cytoplasmic: 163-176
Helical: 177-197
Extracellular: 198-207
Helical: 208-228
Cytoplasmic: 229-235
Helical: 236-256
Extracellular: 257-268
Helical: 269-289
Cytoplasmic: 290-323

Yu, W. B., Ye, Z. H., Chen, X., Shi, J. J., & Lu, J. J. (2021). The development of small-molecule inhibitors targeting CD47. Drug Discovery Today, 26(2), 561-568.

Lin, A., Razzokov, J., Verswyvel, H., Privat-Maldonado, A., De Backer, J., Yusupov, M., ... & Bogaerts, A. (2021). Oxidation of innate immune checkpoint cd47 on cancer cells with non-thermal plasma. Cancers, 13(3), 579.

Wang, F., Liu, Y. H., Zhang, T., Gao, J., Xu, Y., Xie, G. Y., ... & Yang, Y. G. (2020). Aging‐associated changes in CD47 arrangement and interaction with thrombospondin‐1 on red blood cells visualized by super‐resolution imaging. Aging cell, 19(10), e13224.

Nigro, A., Ricciardi, L., Salvato, I., Sabbatino, F., Vitale, M., Crescenzi, M. A., ... & Dal Col, J. (2020). Enhanced expression of CD47 is associated with off-target resistance to tyrosine kinase inhibitor gefitinib in NSCLC. Frontiers in immunology, 10, 3135.

Logtenberg, M. E., Scheeren, F. A., & Schumacher, T. N. (2020). The CD47-SIRPα immune checkpoint. Immunity, 52(5), 742-752.

Tal, M. C., Torrez Dulgeroff, L. B., Myers, L., Cham, L. B., Mayer-Barber, K. D., Bohrer, A. C., ... & Hasenkrug, K. J. (2020). Upregulation of CD47 is a host checkpoint response to pathogen recognition. Mbio, 11(3), e01293-20.

Hayat, S. M. G., Bianconi, V., Pirro, M., & Sahebkar, A. (2019). Stealth functionalization of biomaterials and nanoparticles by CD47 mimicry. International journal of pharmaceutics, 569, 118628.

Petralia, M. C., Mazzon, E., Fagone, P., Russo, A., Longo, A., Avitabile, T., ... & Basile, M. S. (2019). Characterization of the pathophysiological role of CD47 in uveal melanoma. Molecules, 24(13), 2450.

Lian, S., Xie, X., Lu, Y., & Jia, L. (2019). Checkpoint CD47 function on tumor metastasis and immune therapy. OncoTargets and therapy, 12, 9105.

Veillette, A., & Chen, J. (2018). SIRPα–CD47 immune checkpoint blockade in anticancer therapy. Trends in immunology, 39(3), 173-184.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant CD47 protein, hFc Tag (CAT#: V2LY-0526-LY1440)

Cynomolgus, Rhesus Recombinant CD47 protein, ECD, His Tag (CAT#: V2LY-0526-LY1691)

Human Recombinant CD47 proteinECD (CAT#: V2LY-0526-LY2881)

Human Recombinant CD47 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2882)

Human Recombinant CD47 protein, ECD, hFc Tag-1 (CAT#: V2LY-0526-LY2883)

Human Recombinant CD47, High activity protein, ECD, His Tag (CAT#: V2LY-0526-LY2884)

Human Recombinant CD47 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2885)

Human Recombinant CD47 protein, His Tag (CAT#: V2LY-0526-LY2886)

Human Recombinant CD47 protein, Biotin Conjugated, (CAT#: V2LY-0526-LY2887)

Mouse Recombinant CD47 proteinECD (CAT#: V2LY-0526-LY8031)

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Human Recombinant CD47 protein, Biotin Conjugated, (CAT#: V2LY-0526-LY2887)

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Mouse Recombinant CD47 protein, His Tag (CAT#: V2LY-0526-LY8034)

Human Recombinant CD47, High activity protein, ECD, His Tag (CAT#: V2LY-0526-LY2884)

Human Recombinant CD47 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2885)

Mouse Recombinant CD47 protein, His Tag-1 (CAT#: V2LY-0526-LY8035)

Human Recombinant CD47 protein, His Tag (CAT#: V2LY-0526-LY2886)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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