Mouse Recombinant CD8A & CD8B protein, Hetero Dimer , Flag & His Tag-1 (V2LY-0526-LY8080)

Name: Mouse Recombinant CD8A & CD8B protein, Hetero Dimer , Flag & His Tag-1
SKU: V2LY-0526-LY8080
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

Flag & His Tag

Protein Construction

This product is Mouse Recombinant CD8A & CD8B protein, Hetero Dimer , Flag & His Tag consist of Amino Acid: 1-196, 1-175 and predicts a molecular mass of 45.9 kDa.

Molecule Mass

45.9 kDa

Polymeric

Hetero Dimer

Verified

HPLC

Sequence

Amino Acid: 1-196, 1-175

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD8a Molecule

Alternative Names

CD8a Molecule; T-Lymphocyte Differentiation Antigen T8/Leu-2; T-Cell Surface Glycoprotein CD8 Alpha Chain; CD8 Antigen, Alpha Polypeptide (P32); MAL; Leu2 T-Lymphocyte Antigen; OKT8 T-Cell Antigen; T-Cell Antigen Leu2;

Function

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

Biological Process

Antigen processing and presentation Source: UniProtKB
Cell surface receptor signaling pathway Source: GO_Central
Cytotoxic T cell differentiation Source: GO_Central
Immune response Source: UniProtKB
Regulation of immune response Source: Reactome
T cell activation Source: UniProtKB
T cell mediated immunity Source: GO_Central
Transmembrane receptor protein tyrosine kinase signaling pathway Source: UniProtKB

Cellular Location

Isoform 1: Cell membrane. CD8A localizes to lipid rafts only when associated with its partner CD8B.
Isoform 2: Secreted

Involvement in disease

CD8 deficiency, familial (CD8 deficiency): An immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

Topology

Extracellular: 22-182
Helical: 183-203
Cytoplasmic: 204-235

PTM

Palmitoylated, but association with CD8B seems to be more important for the enrichment of CD8A in lipid rafts.
O-glycosylated.
Phosphorylated in cytotoxic T-lymphocytes (CTLs) following activation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD8A protein, APC Conjugated, His Tag (CAT#: V2LY-0526-LY2952)

Human Recombinant CD8A protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2953)

Human Recombinant CD8A protein, ECD, hFc Tag-1 (CAT#: V2LY-0526-LY2954)

Human Recombinant CD8A protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2955)

Human Recombinant CD8A protein, ECD, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2956)

Human Recombinant CD8A protein, ECD, His Tag (CAT#: V2LY-0526-LY2957)

Human Recombinant CD8A protein, PE Conjugated, His Tag (CAT#: V2LY-0526-LY2958)

Cynomolgus Recombinant CD8A protein, ECD, hFc Tag (CAT#: V2LY-0526-LY1450)

Cynomolgus Recombinant CD8A protein, ECD, hFc Tag-1 (CAT#: V2LY-0526-LY1451)

Cynomolgus Recombinant CD8A protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY1452)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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