Mouse Recombinant CEL protein, His Tag (V2LY-0526-LY8098)

Name: Mouse Recombinant CEL protein, His Tag
SKU: V2LY-0526-LY8098
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant CEL protein, His Tag consist of Amino Acid: 1-534 and predicts a molecular mass of 58.2 kDa.

Molecule Mass

58.2 kDa

Sequence

Amino Acid: 1-534

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Carboxyl Ester Lipase

Function

Catalyzes the hydrolysis of a wide range of substrates including cholesteryl esters, phospholipids, lysophospholipids, di- and tri-acylglycerols, and fatty acid esters of hydroxy fatty acids (FAHFAs) (PubMed:8471055, PubMed:27509211, PubMed:10220579, PubMed:27650499).
Preferentially hydrolyzes FAHFAs with the ester bond further away from the carboxylate. Unsaturated FAHFAs are hydrolyzed more quickly than saturated FAHFAs (By similarity).
Has an essential role in the complete digestion of dietary lipids and their intestinal absorption, along with the absorption of fat-soluble vitamins (PubMed:8471055, PubMed:27509211, PubMed:10220579, PubMed:27650499).

Biological Process

Ceramide catabolic process Source: Ensembl
Chemical synaptic transmission Source: GO_Central
Cholesterol catabolic process Source: UniProtKB
Fatty acid catabolic process Source: UniProtKB
Intestinal cholesterol absorption Source: UniProtKB
Intestinal lipid catabolic process Source: UniProtKB
Lipid digestion Source: Reactome
Lipid metabolic process Source: UniProtKB
Modulation of chemical synaptic transmission Source: GO_Central
Neuron cell-cell adhesion Source: GO_Central
Pancreatic juice secretion Source: UniProtKB
Postsynaptic membrane assembly Source: GO_Central
Presynaptic membrane assembly Source: GO_Central
Protein esterification Source: UniProtKB
Synaptic vesicle endocytosis Source: GO_Central

Cellular Location

Secreted

Involvement in disease

Maturity-onset diabetes of the young 8 with exocrine dysfunction (MODY8): The disease is caused by variants affecting the gene represented in this entry. Single base deletions in the VNTR-region, that result in frame shift and protein truncation, have been identified as disease causing variants in MODY8 families (PubMed:16369531). An autosomal dominant form of diabetes characterized by a primary defect in insulin secretion, exocrine pancreatic dysfunction, altered pancreatic morphology, recurrent abdominal pain, and fecal elastase deficiency. Disease onset is at less than 25 years of age.

PTM

N- and O-glycosylated.

Pellegrini, S., Pipitone, G. B., Cospito, A., Manenti, F., Poggi, G., Lombardo, M. T., ... & Piemonti, L. (2021). Generation of β Cells from iPSC of a MODY8 Patient with a Novel Mutation in the Carboxyl Ester Lipase (CEL) Gene. The Journal of Clinical Endocrinology & Metabolism, 106(5), e2322-e2333.

Gravdal, A., Xiao, X., Cnop, M., El Jellas, K., Johansson, S., Njølstad, P. R., ... & Fjeld, K. (2021). The position of single-base deletions in the VNTR sequence of the carboxyl ester lipase (CEL) gene determines proteotoxicity. Journal of Biological Chemistry, 296.

Dalva, M., Lavik, I. K., El Jellas, K., Gravdal, A., Lugea, A., Pandol, S. J., ... & Molven, A. (2020). Pathogenic carboxyl ester lipase (CEL) variants interact with the normal CEL protein in pancreatic cells. Cells, 9(1), 244.

Qiu, Y., Sun, S., Yu, X., Zhou, J., Cai, W., & Qian, L. (2020). Carboxyl ester lipase is highly conserved in utilizing maternal supplied lipids during early development of zebrafish and human. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1865(6), 158663.

Khatua, B., Trivedi, R. N., Noel, P., Patel, K., Singh, R., de Oliveira, C., ... & Singh, V. P. (2019). Carboxyl ester lipase may not mediate lipotoxic injury during severe acute pancreatitis. The American journal of pathology, 189(6), 1226-1240.

Oracz, G., Kujko, A. A., Fjeld, K., Wertheim-Tysarowska, K., Adamus-Białek, W., Steine, S. J., ... & Rygiel, A. M. (2019). The hybrid allele 1 of carboxyl-ester lipase (CEL-HYB1) in Polish pediatric patients with chronic pancreatitis. Pancreatology, 19(4), 531-534.

Cui, Y., Jiao, Y., Wang, K., He, M., & Yang, Z. (2019). A new prognostic factor of breast cancer: High carboxyl ester lipase expression related to poor survival. Cancer genetics, 239, 54-61.

Johansson, B. B., Fjeld, K., El Jellas, K., Gravdal, A., Dalva, M., Tjora, E., ... & Molven, A. (2018). The role of the carboxyl ester lipase (CEL) gene in pancreatic disease. Pancreatology, 18(1), 12-19.

El Jellas, K., Johansson, B. B., Fjeld, K., Antonopoulos, A., Immervoll, H., Choi, M. H., ... & Molven, A. (2018). The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants. Journal of Biological Chemistry, 293(50), 19476-19491.

Dalva, M., El Jellas, K., Steine, S. J., Johansson, B. B., Ringdal, M., Torsvik, J., ... & Molven, A. (2017). Copy number variants and VNTR length polymorphisms of the carboxyl-ester lipase (CEL) gene as risk factors in pancreatic cancer. Pancreatology, 17(1), 83-88.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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