Mouse Anti-CEL Recombinant Antibody (CBXC-0361) (CBMAB-C4597-CQ)

Name: Mouse Anti-CEL Recombinant Antibody (CBXC-0361)
SKU: CBMAB-C4597-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-0361

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 439-475 within an internal region of CEL of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Horse, Dog

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Carboxyl Ester Lipase

Introduction

The protein encoded by this gene is a glycoprotein secreted from the pancreas into the digestive tract and from the lactating mammary gland into human milk. The physiological role of this protein is in cholesterol and lipid-soluble vitamin ester hydrolysis and absorption. This encoded protein promotes large chylomicron production in the intestine. Also its presence in plasma suggests its interactions with cholesterol and oxidized lipoproteins to modulate the progression of atherosclerosis. In pancreatic tumoral cells, this encoded protein is thought to be sequestrated within the Golgi compartment and is probably not secreted. This gene contains a variable number of tandem repeat (VNTR) polymorphism in the coding region that may influence the function of the encoded protein.

Entrez Gene ID

1056

UniProt ID

P19835

Alternative Names

Carboxyl Ester Lipase; Bile Salt-Stimulated Lipase; Cholesterol Esterase; Sterol Esterase; Bucelipase; BSSL; BAL; Carboxyl Ester Lipase (Bile Salt-Stimulated Lipase); Bile Salt-Dependent Lipase, Oncofetal Isoform; Fetoacinar Pancreatic Protein; Lysophospholipase, Pancreatic; Pancreatic Lysophospholipase; Bile Salt-Activated Lipase;

Function

Catalyzes the hydrolysis of a wide range of substrates including cholesteryl esters, phospholipids, lysophospholipids, di- and tri-acylglycerols, and fatty acid esters of hydroxy fatty acids (FAHFAs) (PubMed:8471055, PubMed:27509211, PubMed:10220579, PubMed:27650499).
Preferentially hydrolyzes FAHFAs with the ester bond further away from the carboxylate. Unsaturated FAHFAs are hydrolyzed more quickly than saturated FAHFAs (By similarity).
Has an essential role in the complete digestion of dietary lipids and their intestinal absorption, along with the absorption of fat-soluble vitamins (PubMed:8471055, PubMed:27509211, PubMed:10220579, PubMed:27650499).

Biological Process

Ceramide catabolic process Source: Ensembl
Chemical synaptic transmission Source: GO_Central
Cholesterol catabolic process Source: UniProtKB
Fatty acid catabolic process Source: UniProtKB
Intestinal cholesterol absorption Source: UniProtKB
Intestinal lipid catabolic process Source: UniProtKB
Lipid digestion Source: Reactome
Lipid metabolic process Source: UniProtKB
Modulation of chemical synaptic transmission Source: GO_Central
Neuron cell-cell adhesion Source: GO_Central
Pancreatic juice secretion Source: UniProtKB
Postsynaptic membrane assembly Source: GO_Central
Presynaptic membrane assembly Source: GO_Central
Protein esterification Source: UniProtKB
Synaptic vesicle endocytosis Source: GO_Central

Cellular Location

Secreted

Involvement in disease

Maturity-onset diabetes of the young 8 with exocrine dysfunction (MODY8): The disease is caused by variants affecting the gene represented in this entry. Single base deletions in the VNTR-region, that result in frame shift and protein truncation, have been identified as disease causing variants in MODY8 families (PubMed:16369531). An autosomal dominant form of diabetes characterized by a primary defect in insulin secretion, exocrine pancreatic dysfunction, altered pancreatic morphology, recurrent abdominal pain, and fecal elastase deficiency. Disease onset is at less than 25 years of age.

PTM

N- and O-glycosylated.

Pellegrini, S., Pipitone, G. B., Cospito, A., Manenti, F., Poggi, G., Lombardo, M. T., ... & Piemonti, L. (2021). Generation of β Cells from iPSC of a MODY8 Patient with a Novel Mutation in the Carboxyl Ester Lipase (CEL) Gene. The Journal of Clinical Endocrinology & Metabolism, 106(5), e2322-e2333.

Gravdal, A., Xiao, X., Cnop, M., El Jellas, K., Johansson, S., Njølstad, P. R., ... & Fjeld, K. (2021). The position of single-base deletions in the VNTR sequence of the carboxyl ester lipase (CEL) gene determines proteotoxicity. Journal of Biological Chemistry, 296.

Dalva, M., Lavik, I. K., El Jellas, K., Gravdal, A., Lugea, A., Pandol, S. J., ... & Molven, A. (2020). Pathogenic carboxyl ester lipase (CEL) variants interact with the normal CEL protein in pancreatic cells. Cells, 9(1), 244.

Qiu, Y., Sun, S., Yu, X., Zhou, J., Cai, W., & Qian, L. (2020). Carboxyl ester lipase is highly conserved in utilizing maternal supplied lipids during early development of zebrafish and human. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1865(6), 158663.

Khatua, B., Trivedi, R. N., Noel, P., Patel, K., Singh, R., de Oliveira, C., ... & Singh, V. P. (2019). Carboxyl ester lipase may not mediate lipotoxic injury during severe acute pancreatitis. The American journal of pathology, 189(6), 1226-1240.

Oracz, G., Kujko, A. A., Fjeld, K., Wertheim-Tysarowska, K., Adamus-Białek, W., Steine, S. J., ... & Rygiel, A. M. (2019). The hybrid allele 1 of carboxyl-ester lipase (CEL-HYB1) in Polish pediatric patients with chronic pancreatitis. Pancreatology, 19(4), 531-534.

Cui, Y., Jiao, Y., Wang, K., He, M., & Yang, Z. (2019). A new prognostic factor of breast cancer: High carboxyl ester lipase expression related to poor survival. Cancer genetics, 239, 54-61.

Johansson, B. B., Fjeld, K., El Jellas, K., Gravdal, A., Dalva, M., Tjora, E., ... & Molven, A. (2018). The role of the carboxyl ester lipase (CEL) gene in pancreatic disease. Pancreatology, 18(1), 12-19.

El Jellas, K., Johansson, B. B., Fjeld, K., Antonopoulos, A., Immervoll, H., Choi, M. H., ... & Molven, A. (2018). The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO blood group determinants. Journal of Biological Chemistry, 293(50), 19476-19491.

Dalva, M., El Jellas, K., Steine, S. J., Johansson, B. B., Ringdal, M., Torsvik, J., ... & Molven, A. (2017). Copy number variants and VNTR length polymorphisms of the carboxyl-ester lipase (CEL) gene as risk factors in pancreatic cancer. Pancreatology, 17(1), 83-88.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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