Mouse Recombinant CRELD2 protein, His Tag (V2LY-0526-LY8161)

Name: Mouse Recombinant CRELD2 protein, His Tag
SKU: V2LY-0526-LY8161
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant CRELD2 protein, His Tag consist of Amino Acid: 1-350 and predicts a molecular mass of 37.5 kDa.

Molecule Mass

37.5 kDa

Sequence

Amino Acid: 1-350

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, NaCl

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Cysteine Rich With EGF Like Domains 2

Function

Protein disulfide isomerase (By similarity).

Might play a role in the unfolded protein response (By similarity).

May regulate transport of alpha4-beta2 neuronal acetylcholine receptor (PubMed:16238698).

Cellular Location

Endoplasmic reticulum

Lasić, V., Kosović, I., Jurić, M., Racetin, A., Čurčić, J., Šolić, I., ... & Vukojević, K. (2021). GREB1L, CRELD2 and ITGA10 expression in the human developmental and postnatal kidneys: an immunohistochemical study. Acta Histochemica, 123(2), 151679.

Boyle, S. T., Poltavets, V., Kular, J., Pyne, N. T., Sandow, J. J., Lewis, A. C., ... & Samuel, M. S. (2020). ROCK-mediated selective activation of PERK signalling causes fibroblast reprogramming and tumour progression through a CRELD2-dependent mechanism. Nature Cell Biology, 22(7), 882-895.

Dennis, E. P., Edwards, S. M., Jackson, R. M., Hartley, C. L., Tsompani, D., Capulli, M., ... & Briggs, M. D. (2020). CRELD2 is a novel LRP1 chaperone that regulates noncanonical WNT signaling in skeletal development. Journal of Bone and Mineral Research, 35(8), 1452-1469.

Chen, M. F., Chang, C. H., Yang, L. Y., Hsieh, P. H., Shih, H. N., Ueng, S. W. N., & Chang, Y. (2019). Synovial fluid interleukin-16, interleukin-18, and CRELD2 as novel biomarkers of prosthetic joint infections. Bone & Joint Research, 8(4), 179-188.

Yu, N., Wang, X., Bao, H., & Liu, Z. (2019). Identification and functional study of three nAChR regulators, ubiquilin‐1, PICK 1, and CRELD 2, in Locusta migratoria manilensis dorsal unpaired median neurons. Journal of Neurochemistry, 149(3), 331-345.

Oh-Hashi, K., Fujimura, K., Norisada, J., & Hirata, Y. (2018). Expression analysis and functional characterization of the mouse cysteine-rich with EGF-like domains 2. Scientific reports, 8(1), 1-11.

Kim, Y., Park, S. J., Manson, S. R., Molina, C. A., Kidd, K., Thiessen-Philbrook, H., ... & Chen, Y. M. (2017). Elevated urinary CRELD2 is associated with endoplasmic reticulum stress–mediated kidney disease. JCI insight, 2(23).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant CRELD2 protein, hFc Tag (CAT#: V2LY-0526-LY8160)

Related Products

Mouse Anti-CRELD2 Recombinant Antibody (C4712) (CAT#: V2LY-1206-LY1136)

Mouse Recombinant CRELD2 protein, hFc Tag (CAT#: V2LY-0526-LY8160)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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