Mouse Recombinant CSF3 protein (V2LY-0526-LY8353)

Name: Mouse Recombinant CSF3 protein
SKU: V2LY-0526-LY8353
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Protein Construction

This product is Mouse Recombinant CSF3 protein consist of Amino Acid: 1-208 and predicts a molecular mass of 19 kDa.

Molecule Mass

19 kDa

Verified

HPLC

Sequence

Amino Acid: 1-208

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.>95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Colony Stimulating Factor 3

Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Biological Process

Cellular response to cytokine stimulus Source: BHF-UCL
Cellular response to lipopolysaccharide Source: Ensembl
Cytokine-mediated signaling pathway Source: Reactome
Granulocyte differentiation Source: UniProtKB
Immune response Source: InterPro
Multicellular organism development Source: ProtInc
Negative regulation of neuron death Source: Ensembl
Positive regulation of actin cytoskeleton reorganization Source: BHF-UCL
Positive regulation of actin filament polymerization Source: BHF-UCL
Positive regulation of cell population proliferation Source: GO_Central
Positive regulation of DNA-binding transcription factor activity Source: BHF-UCL
Positive regulation of myeloid cell differentiation Source: GO_Central
Positive regulation of peptidyl-serine phosphorylation Source: BHF-UCL
Positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
Positive regulation of phosphatidylinositol 3-kinase signaling Source: BHF-UCL
Positive regulation of protein binding Source: BHF-UCL
Positive regulation of protein kinase B signaling Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Response to ethanol Source: Ensembl

Cellular Location

Secreted

PTM

O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

Saunders, A. S., Bender, D. E., Ray, A. L., Wu, X., & Morris, K. T. (2021). Colony-stimulating factor 3 signaling in colon and rectal cancers: Immune response and CMS classification in TCGA data. Plos one, 16(2), e0247233.

Jannaman, E. A., Xiao, Y., & Hansen, P. J. (2020). Actions of colony-stimulating factor 3 on the maturing oocyte and developing embryo in cattle. Journal of Animal Science, 98(4), skaa115.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CSF3 (Isoform b) protein (CAT#: V2LY-0526-LY4277)

Human Recombinant CSF3 (Isoform b) protein-1 (CAT#: V2LY-0526-LY4278)

Human Recombinant CSF3 (Isoform b) protein, hFc Tag (CAT#: V2LY-0526-LY4279)

Related Products

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Mouse Anti-CSF3 Recombinant Antibody (CBFYH-0109) (CAT#: V2LY-1206-LY1464)

Mouse Anti-CSF3 Recombinant Antibody (CBFYH-2862) (CAT#: V2LY-1206-LY1466)

Human Recombinant CSF3 (Isoform b) protein-1 (CAT#: V2LY-0526-LY4278)

Human Recombinant CSF3 (Isoform b) protein (CAT#: V2LY-0526-LY4277)

Mouse Anti-CSF3 Recombinant Antibody (CBT4377) (CAT#: V2LY-0625-LY221)

Mouse Anti-CSF3 Recombinant Antibody (2C5) (CAT#: V2LY-1206-LY1474)

Mouse Anti-CSF3 Recombinant Antibody (CBFYH-0107) (CAT#: V2LY-1206-LY1469)

Rat Anti-CSF3 Recombinant Antibody (G1451) (CAT#: CBMAB-G1451-LY)

Rabbit Anti-CSF3 Recombinant Antibody (CBLG1-2486) (CAT#: V2LY-1206-LY1476)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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