Mouse Recombinant CST3 protein, His Tag (V2LY-0526-LY8185)

Name: Mouse Recombinant CST3 protein, His Tag
SKU: V2LY-0526-LY8185
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant CST3 protein, His Tag consist of Amino Acid: 1-140 and predicts a molecular mass of 15 kDa.

Molecule Mass

15 kDa

Sequence

Amino Acid: 1-140

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, NaCl

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Cystatin C

Alternative Names

Cystatin C; Neuroendocrine Basic Polypeptide; Post-Gamma-Globulin; Gamma-Trace; Cystatin C (Amyloid Angiopathy And Cerebral Hemorrhage); Epididymis Secretory Protein Li 2; BA218C14.4 (Cystatin C);

Function

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

Biological Process

Amyloid fibril formation Source: Reactome
Cellular protein metabolic process Source: Reactome
Defense response Source: BHF-UCL
Negative regulation of blood vessel remodeling Source: BHF-UCL
Negative regulation of collagen catabolic process Source: BHF-UCL
Negative regulation of elastin catabolic process Source: BHF-UCL
Negative regulation of extracellular matrix disassembly Source: BHF-UCL
Negative regulation of peptidase activity Source: ARUK-UCL
Negative regulation of proteolysis Source: UniProtKB
Neutrophil degranulation Source: Reactome
Post-translational protein modification Source: Reactome
Regulation of tissue remodeling Source: BHF-UCL
Supramolecular fiber organization Source: BHF-UCL

Cellular Location

Secreted

Involvement in disease

Amyloidosis 6 (AMYL6):
A hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low.
Macular degeneration, age-related, 11 (ARMD11):
A form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.

PTM

The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.

Zhang, X., Liu, X., Su, G., Li, M., Liu, J., Wang, C., & Xu, D. (2021). pH-dependent and dynamic interactions of cystatin C with heparan sulfate. Communications biology, 4(1), 1-11.

Christopher, J. R., Ponnaiyan, D., Parthasarathy, H., & Tadepalli, A. (2021). Association of CST3 Gene with Its Protein: Cystatin C in Health and Severe Periodontal Disease. Genetic Testing and Molecular Biomarkers, 25(6), 405-410.

Indacochea, A., Guerrero, S., Ureña, M., Araujo, F., Coll, O., LLeonart, M. E., & Gebauer, F. (2021). Cold-inducible RNA binding protein promotes breast cancer cell malignancy by regulating Cystatin C levels. Rna, 27(2), 190-201.

Tahir, N. A. M., Saffian, S. M., Islahudin, F. H., Gafor, A. H. A., Othman, H., Manan, H. A., & Makmor-Bakry, M. (2020). Effects of CST3 Gene G73A Polymorphism on Cystatin C in a Prospective Multiethnic Cohort Study. Nephron, 144(4), 204-212.

Hoghooghi, V., Palmer, A. L., Frederick, A., Jiang, Y., Merkens, J. E., Balakrishnan, A., ... & Ousman, S. S. (2020). Cystatin C Plays a Sex-Dependent Detrimental Role in Experimental Autoimmune Encephalomyelitis. Cell reports, 33(1), 108236.

Maniwa, K., Yano, S., Sheikh, A. M., Onoda, K., Mitaki, S., Isomura, M., ... & Nagai, A. (2020). Association between cystatin C gene polymorphism and the prevalence of white matter lesion in elderly healthy subjects. Scientific Reports, 10(1), 1-9.

Huda, M. N., VerHague, M., Albright, J., Smallwood, T., Bell, T. A., Que, E., ... & Bennett, B. J. (2020). Dissecting the genetic architecture of cystatin C in Diversity Outbred mice. G3: Genes, Genomes, Genetics, 10(7), 2529-2541.

Zeng, Q., Huang, Z., Wei, L., Fang, J., & Lin, K. (2019). Correlations of serum cystatin C level and gene polymorphism with vascular cognitive impairment after acute cerebral infarction. Neurological Sciences, 40(5), 1049-1054.

Lee, R. K. K., Tseng, H. C., Hwu, Y. M., Fan, C. C., Lin, M. H., Yu, J. J., ... & Li, S. H. (2018). Expression of cystatin C in the female reproductive tract and its effect on human sperm capacitation. Reproductive Biology and Endocrinology, 16(1), 1-10.

Li, Z., Wang, S., Huo, X., Yu, H., Lu, J., Zhang, S., ... & Chen, Z. (2018). Cystatin C expression is promoted by VEGFA blocking, with inhibitory effects on endothelial cell angiogenic functions including proliferation, migration, and chorioallantoic membrane angiogenesis. Journal of the American Heart Association, 7(21), e009167.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Recombinant CST3 protein, His Tag (CAT#: V2LY-0526-LY9321)

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Isotype control

For research use only. Not intended for any clinical use.

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