Mouse Recombinant IDO protein, His Tag (V2LY-0526-LY8423)

Name: Mouse Recombinant IDO protein, His Tag
SKU: V2LY-0526-LY8423
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant IDO protein, His Tag consist of Amino Acid: 1-407 and predicts a molecular mass of 46.47 kDa.

Molecule Mass

46.47 kDa

Sequence

Amino Acid: 1-407

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Indoleamine 2,3-Dioxygenase 1

Function

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174).

Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885).

Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885).

Acts as a suppressor of anti-tumor immunity (PubMed:23103127, PubMed:25157255, PubMed:14502282, PubMed:25691885).

Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885).

Protects the fetus from maternal immune rejection (PubMed:25691885).

Biological Process

de novo' NAD biosynthetic process from tryptophan Source: GO_Central
Female pregnancy Source: ProtInc
Immune system process Source: UniProtKB-KW
Inflammatory response Source: Ensembl
Kynurenic acid biosynthetic process Source: Ensembl
Multicellular organismal response to stress Source: Ensembl
Negative regulation of interleukin-10 production Source: Ensembl
Negative regulation of T cell apoptotic process Source: Ensembl
Negative regulation of T cell proliferation Source: Ensembl
Positive regulation of chronic inflammatory response Source: Ensembl
Positive regulation of interleukin-12 production Source: Ensembl
Positive regulation of T cell apoptotic process Source: UniProtKB
Positive regulation of T cell tolerance induction Source: Ensembl
Positive regulation of type 2 immune response Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Swimming behavior Source: Ensembl
Tryptophan catabolic process Source: ProtInc
Tryptophan catabolic process to kynurenine Source: GO_Central

Cellular Location

Cytosol

Salminen, A. (2022). Role of indoleamine 2, 3-dioxygenase 1 (IDO1) and kynurenine pathway in the regulation of the aging process. Ageing Research Reviews, 75, 101573.

Pallotta, M. T., Rossini, S., Suvieri, C., Coletti, A., Orabona, C., Macchiarulo, A., ... & Grohmann, U. (2022). Indoleamine 2, 3‐dioxygenase 1 (IDO1): an up‐to‐date overview of an eclectic immunoregulatory enzyme. The FEBS journal, 289(20), 6099-6118.

Fujiwara, Y., Kato, S., Nesline, M. K., Conroy, J. M., DePietro, P., Pabla, S., & Kurzrock, R. (2022). Indoleamine 2, 3-dioxygenase (IDO) inhibitors and cancer immunotherapy. Cancer Treatment Reviews, 102461.

Zhang, Y., Hu, Z., Zhang, J., Ren, C., & Wang, Y. (2022). Dual-target inhibitors of indoleamine 2, 3 dioxygenase 1 (Ido1): A promising direction in cancer immunotherapy. European Journal of Medicinal Chemistry, 238, 114524.

Tang, K., Wu, Y. H., Song, Y., & Yu, B. (2021). Indoleamine 2, 3-dioxygenase 1 (IDO1) inhibitors in clinical trials for cancer immunotherapy. Journal of Hematology & Oncology, 14, 1-21.

Röhrig, U. F., Michielin, O., & Zoete, V. (2021). Structure and plasticity of indoleamine 2, 3-dioxygenase 1 (IDO1). Journal of Medicinal Chemistry, 64(24), 17690-17705.

Wang, X. X., Sun, S. Y., Dong, Q. Q., Wu, X. X., Tang, W., & Xing, Y. Q. (2019). Recent advances in the discovery of indoleamine 2, 3-dioxygenase 1 (IDO1) inhibitors. MedChemComm, 10(10), 1740-1754.

Röhrig, U. F., Reynaud, A., Majjigapu, S. R., Vogel, P., Pojer, F., & Zoete, V. (2019). Inhibition mechanisms of indoleamine 2, 3-dioxygenase 1 (IDO1). Journal of medicinal chemistry, 62(19), 8784-8795.

Nayak-Kapoor, A., Hao, Z., Sadek, R., Dobbins, R., Marshall, L., Vahanian, N. N., ... & Khleif, S. N. (2018). Phase Ia study of the indoleamine 2, 3-dioxygenase 1 (IDO1) inhibitor navoximod (GDC-0919) in patients with recurrent advanced solid tumors. Journal for immunotherapy of cancer, 6(1), 1-12.

Komiya, T., & Huang, C. H. (2018). Updates in the clinical development of epacadostat and other indoleamine 2, 3-dioxygenase 1 inhibitors (IDO1) for human cancers. Frontiers in oncology, 8, 423.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IDO protein (CAT#: V2LY-0526-LY4639)

Human Recombinant IDO protein, GST Tag (CAT#: V2LY-0526-LY4640)

IDO1 Matched Antibody Pair (585) (CAT#: APMAB-585LY)

IDO1 Matched Antibody Pair (586) (CAT#: APMAB-586LY)

Antibody Pairs

IDO1 Matched Antibody Pair (585) (CAT#: APMAB-585LY)

IDO1 Matched Antibody Pair (586) (CAT#: APMAB-586LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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