Mouse Recombinant IL34 protein, His Tag (V2LY-0526-LY8538)

Name: Mouse Recombinant IL34 protein, His Tag
SKU: V2LY-0526-LY8538
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant IL34 protein, His Tag consist of Amino Acid: 1-235 and predicts a molecular mass of 26 kDa.

Molecule Mass

26 kDa

Sequence

Amino Acid: 1-235

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Interleukin 34

Function

Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.

Biological Process

Inflammatory responseIEA:UniProtKB-KW
Innate immune responseIEA:UniProtKB-KW
Interleukin-34-mediated signaling pathwayManual Assertion Based On ExperimentIDA:ARUK-UCL
Microglial cell proliferationIEA:Ensembl
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of gene expressionIEA:Ensembl
Positive regulation of macrophage chemotaxisManual Assertion Based On ExperimentIDA:ARUK-UCL
Positive regulation of macrophage differentiationManual Assertion Based On ExperimentIDA:CACAO
Positive regulation of macrophage proliferationManual Assertion Based On ExperimentIDA:ARUK-UCL
Positive regulation of MAP kinase activityManual Assertion Based On ExperimentIDA:ARUK-UCL
Positive regulation of monocyte differentiationManual Assertion Based On ExperimentIDA:CACAO
Positive regulation of oligodendrocyte differentiationISS:UniProtKB
Positive regulation of protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of protein tyrosine kinase activityManual Assertion Based On ExperimentIDA:ARUK-UCL

Cellular Location

Secreted

Freuchet, A., Salama, A., Bézie, S., Tesson, L., Rémy, S., Humeau, R., ... & Guillonneau, C. (2022). IL‐34 deficiency impairs FOXP3+ Treg function in a model of autoimmune colitis and decreases immune tolerance homeostasis. Clinical and Translational Medicine, 12(8), e988.

Duarte, C., Akkaoui, J., Ho, A., Garcia, C., Yamada, C., & Movila, A. (2021). Age-dependent effects of the recombinant spike protein/SARS-CoV-2 on the M–CSF–and IL-34-differentiated macrophages in vitro. Biochemical and Biophysical Research Communications, 546, 97-102.

Freuchet, A., Salama, A., Remy, S., Guillonneau, C., & Anegon, I. (2021). IL-34 and CSF-1, deciphering similarities and differences at steady state and in diseases. Journal of Leukocyte Biology, 110(4), 771-796.

Otsuka, R., Wada, H., & Seino, K. I. (2021, April). IL-34, the rationale for its expression in physiological and pathological conditions. In Seminars in Immunology (Vol. 54, p. 101517). Academic Press.

Lelios, I., Cansever, D., Utz, S. G., Mildenberger, W., Stifter, S. A., & Greter, M. (2020). Emerging roles of IL-34 in health and disease. Journal of Experimental Medicine, 217(3), e20190290.

Blondy, T., d'Almeida, S. M., Briolay, T., Tabiasco, J., Meiller, C., Chéné, A. L., ... & Blanquart, C. (2020). Involvement of the M-CSF/IL-34/CSF-1R pathway in malignant pleural mesothelioma. Journal for immunotherapy of cancer, 8(1).

Su, Y., Yu, Y., Liu, C., Zhang, Y., Liu, C., Ge, M., ... & Meng, Q. (2020). Fate decision of satellite cell differentiation and self-renewal by miR-31-IL34 axis. Cell Death & Differentiation, 27(3), 949-965.

Lin, W., Xu, D., Austin, C. D., Caplazi, P., Senger, K., Sun, Y., ... & Zarrin, A. A. (2019). Function of CSF1 and IL34 in macrophage homeostasis, inflammation, and cancer. Frontiers in immunology, 10, 2019.

Easley-Neal, C., Foreman, O., Sharma, N., Zarrin, A. A., & Weimer, R. M. (2019). CSF1R ligands IL-34 and CSF1 are differentially required for microglia development and maintenance in white and gray matter brain regions. Frontiers in immunology, 10, 2199.

Zhao, Z., Pan, G., Tang, C., Li, Z., Zheng, D., Wei, X., & Wu, Z. (2018). IL-34 inhibits acute rejection of rat liver transplantation by inducing Kupffer cell M2 polarization. Transplantation, 102(6), e265-e274.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IL34 protein, His Tag (CAT#: V2LY-0526-LY4899)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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