Mouse Recombinant IL36B protein (V2LY-0526-LY8540)

Name: Mouse Recombinant IL36B protein
SKU: V2LY-0526-LY8540
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Mouse

Protein Construction

This product is Mouse Recombinant IL36B protein consist of Amino Acid: 1-183 and predicts a molecular mass of 21 kDa.

Molecule Mass

21 kDa

Sequence

Amino Acid: 1-183

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>96% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Interleukin 36 Beta

Function

Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6.

Biological Process

Cellular response to lipopolysaccharideManual Assertion Based On ExperimentIBA:GO_Central
Cytokine-mediated signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Immune response1 PublicationNAS:UniProtKB
Inflammatory responseManual Assertion Based On ExperimentIBA:GO_Central
Inflammatory response to antigenic stimulusManual Assertion Based On ExperimentIBA:GO_Central
Innate immune responseIEA:UniProtKB-KW
Positive regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of T cell differentiationManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm; Secreted. The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion.

PTM

N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IL36B protein (CAT#: V2LY-0526-LY4906)

Human Recombinant IL36B protein, His Tag (CAT#: V2LY-0526-LY4907)

Human IL36B ELISA Kit (CAT#: V2LY-0626-LY4167)

Mouse Il36b ELISA Kit (CAT#: V2LY-0626-LY5818)

Related Products

Human Recombinant IL36B protein (CAT#: V2LY-0526-LY4906)

Mouse Anti-IL36B Recombinant Antibody (1E4) (CAT#: CBMAB-A4375-LY)

Human Recombinant IL36B protein, His Tag (CAT#: V2LY-0526-LY4907)

Mouse Anti-IL36B Recombinant Antibody (CF230) (CAT#: CBMAB-FT268LY)

Mouse Il36b ELISA Kit (CAT#: V2LY-0626-LY5818)

Mouse Anti-IL36B Recombinant Antibody (A518) (CAT#: CBMAB-AP10853LY)

Human IL36B ELISA Kit (CAT#: V2LY-0626-LY4167)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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