Mouse Recombinant LAG3 protein, hFc Tag (V2LY-0526-LY8602)

Name: Mouse Recombinant LAG3 protein, hFc Tag
SKU: V2LY-0526-LY8602
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

hFc Tag

Protein Construction

This product is Mouse Recombinant LAG3 protein, hFc Tag consist of Amino Acid: 1-432 and predicts a molecular mass of 71.1 kDa.

Molecule Mass

71.1 kDa

Sequence

Amino Acid: 1-432

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Arginine, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

lymphocyte activating 3

Alternative Names

lymphocyte activating 3

Function

Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed:7805750, PubMed:8647185, PubMed:20421648).
Delivers inhibitory signals upon binding to ligands, such as FGL1 (By similarity).
FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (By similarity).
Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity).
May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (By similarity).
Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed:7805750, PubMed:8647185, PubMed:20421648).
Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (By similarity).
Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (By similarity).
Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed:8647185).
Secreted lymphocyte activation gene 3 protein
May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response.

Biological Process

Adaptive immune responseIEA:UniProtKB-KW
Cell surface receptor signaling pathwayISS:UniProtKB
Negative regulation of interleukin-2 productionIEA:Ensembl
Negative regulation of regulatory T cell differentiationISS:UniProtKB
Plasmacytoid dendritic cell activationISS:UniProtKB
Positive regulation of natural killer cell mediated cytotoxicityIEA:Ensembl
Regulation of immune responseISS:UniProtKB

Cellular Location

Lymphocyte activation gene 3 protein:
Cell membrane
Secreted lymphocyte activation gene 3 protein:
Secreted
Produced following cleavage of the main chain.

Topology

Extracellular: 23-450
Helical: 451-471
Cytoplasmic: 472-525

PTM

Lymphocyte activation gene 3 protein
Proteolytically cleaved by ADAM10 and ADAM17 within the connecting peptide region, leading to release of Secreted lymphocyte activation gene 3 protein (sLAG-3). ADAM10 mediates constitutive cleavage, but cleavage increases following T-cell activation, whereas shedding by ADAM17 is induced by TCR signaling in a PRKCQ-dependent manner.

Zhao, L., Wang, H., Xu, K., Liu, X., & He, Y. (2022). Update on lymphocyte-activation gene 3 (LAG-3) in cancers: From biological properties to clinical applications. Chinese Medical Journal, 135(10), 1203-1212.

Lythgoe, M. P., Liu, D. S. K., Annels, N. E., Krell, J., & Frampton, A. E. (2021). Gene of the month: Lymphocyte-activation gene 3 (LAG-3). Journal of Clinical Pathology, 74(9), 543-547.

Slevin, S. M., Garner, L. C., Lahiff, C., Tan, M., Wang, L. M., Ferry, H., ... & Keshav, S. (2020). Lymphocyte activation gene (LAG)-3 is associated with mucosal inflammation and disease activity in ulcerative colitis. Journal of Crohn's and Colitis, 14(10), 1446-1461.

Gebauer, F., Krämer, M., Bruns, C., Schlößer, H. A., Thelen, M., Lohneis, P., ... & Quaas, A. (2020). Lymphocyte activation gene-3 (LAG3) mRNA and protein expression on tumour infiltrating lymphocytes (TILs) in oesophageal adenocarcinoma. Journal of cancer research and clinical oncology, 146, 2319-2327.

Atkinson, V., Khattak, A., Haydon, A., Eastgate, M., Roy, A., Prithviraj, P., ... & Triebel, F. (2020). Eftilagimod alpha, a soluble lymphocyte activation gene-3 (LAG-3) protein plus pembrolizumab in patients with metastatic melanoma. Journal for immunotherapy of cancer, 8(2).

Yu, X., Huang, X., Chen, X., Liu, J., Wu, C., Pu, Q., ... & Zhou, L. (2019, August). Characterization of a novel anti-human lymphocyte activation gene 3 (LAG-3) antibody for cancer immunotherapy. In MAbs (Vol. 11, No. 6, pp. 1139-1148). Taylor & Francis.

Ruffo, E., Wu, R. C., Bruno, T. C., Workman, C. J., & Vignali, D. A. (2019, April). Lymphocyte-activation gene 3 (LAG3): The next immune checkpoint receptor. In Seminars in immunology (Vol. 42, p. 101305). Academic Press.

Saleh, R. R., Peinado, P., Fuentes-Antrás, J., Pérez-Segura, P., Pandiella, A., Amir, E., & Ocaña, A. (2019). Prognostic value of lymphocyte-activation gene 3 (LAG3) in cancer: a meta-analysis. Frontiers in oncology, 9, 1040.

Zhang, Y., Liu, Y. D., Luo, Y. L., Liu, B. L., Huang, Q. T., Wang, F., & Zhong, Q. (2018). Prognostic value of lymphocyte activation gene-3 (LAG-3) expression in esophageal squamous cell carcinoma. Journal of Cancer, 9(22), 4287.

Wang, Y., Dong, T., Xuan, Q., Zhao, H., Qin, L., & Zhang, Q. (2018). Lymphocyte-activation gene-3 expression and prognostic value in neoadjuvant-treated triple-negative breast cancer. Journal of breast cancer, 21(2), 124-133.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant LAG3 protein, His Tag (CAT#: V2LY-0526-LY1591)

Human Recombinant LAG3 proteinECD (CAT#: V2LY-0526-LY5231)

Human Recombinant LAG3 proteinECD, Biotin Conjugated, (CAT#: V2LY-0526-LY5232)

Human Recombinant LAG3 proteinECD-1 (CAT#: V2LY-0526-LY5233)

Human Recombinant LAG3 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY5234)

Human Recombinant LAG3 protein, ECD, His Tag (CAT#: V2LY-0526-LY5235)

Human Recombinant LAG3 protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY5236)

Human Recombinant LAG3 protein, ECD, Fc Tag (CAT#: V2LY-0526-LY5237)

Human Recombinant LAG3 protein, ECD, Fc Tag-1 (CAT#: V2LY-0526-LY5238)

Human Recombinant LAG3 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY5239)

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For research use only. Not intended for any clinical use.

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