Mouse Recombinant S100A8 protein, His Tag (V2LY-0526-LY8863)

Name: Mouse Recombinant S100A8 protein, His Tag
SKU: V2LY-0526-LY8863
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant S100A8 protein, His Tag consist of Amino Acid: 1-89 and predicts a molecular mass of 11.7 kDa.

Molecule Mass

11.7 kDa

Sequence

Amino Acid: 1-89

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, Glycerol, DTT

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

S100 Calcium Binding Protein A8

Function

S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its pro-inflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.
(Microbial infection) Upon infection by human coronavirus SARS-CoV-2, may induce expansion of aberrant immature neutrophils in a TLR4-dependent manner.

Biological Process

Biological Process activation of cysteine-type endopeptidase activity involved in apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process acute inflammatory responseIEA:Ensembl
Biological Process apoptotic processIEA:UniProtKB-KW
Biological Process astrocyte developmentIEA:Ensembl
Biological Process autocrine signalingIEA:Ensembl
Biological Process autophagyManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process chronic inflammatory responseIEA:Ensembl
Biological Process defense response to bacteriumManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process defense response to fungusManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process inflammatory responseManual Assertion Based On ExperimentTAS:ProtInc
Biological Process innate immune responseIEA:UniProtKB-KW
Biological Process leukocyte migration involved in inflammatory responseManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process neutrophil aggregationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process neutrophil chemotaxisManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process peptide secretionIEA:Ensembl
Biological Process peptidyl-cysteine S-nitrosylationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of cell growthManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process positive regulation of inflammatory responseManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of intrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process positive regulation of peptide secretionIEA:Ensembl
Biological Process regulation of cytoskeleton organizationManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process response to ethanolIEA:Ensembl
Biological Process response to lipopolysaccharideManual Assertion Based On ExperimentIBA:GO_Central
Biological Process response to zinc ionIEA:Ensembl
Biological Process sequestering of zinc ionManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process wound healingIEA:Ensembl

Cellular Location

Secreted
Cytoplasm
Cytoplasm, cytoskeleton
Cell membrane
Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation or endothelial adhesion of monocytes, is secreted via a microtubule-mediated, alternative pathway.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant S100A8 protein, His Tag (CAT#: V2LY-0526-LY6656)

Human Recombinant S100A8 protein, His Tag-1 (CAT#: V2LY-0526-LY6657)

Mouse Recombinant S100A8 protein, His Tag-1 (CAT#: V2LY-0526-LY8864)

Human Recombinant S100A8 & S100A9 protein, Hetero Dimer , Flag & His Tag (CAT#: V2LY-0526-LY6655)

S100A8 Matched Antibody Pair (1026) (CAT#: APMAB-1026LY)

S100A8 Matched Antibody Pair (1027) (CAT#: APMAB-1027LY)

S100A8 Matched Antibody Pair (1028) (CAT#: APMAB-1028LY)

S100A8 Matched Antibody Pair (1029) (CAT#: APMAB-1029LY)

S100A8 Matched Antibody Pair (1030) (CAT#: APMAB-1030LY)

S100A8 Matched Antibody Pair (1031) (CAT#: APMAB-1031LY)

Antibody Pairs

S100A8 Matched Antibody Pair (1026) (CAT#: APMAB-1026LY)

S100A8 Matched Antibody Pair (1027) (CAT#: APMAB-1027LY)

S100A8 Matched Antibody Pair (1028) (CAT#: APMAB-1028LY)

S100A8 Matched Antibody Pair (1029) (CAT#: APMAB-1029LY)

S100A8 Matched Antibody Pair (1030) (CAT#: APMAB-1030LY)

S100A8 Matched Antibody Pair (1031) (CAT#: APMAB-1031LY)

S100A8 Matched Antibody Pair (1032) (CAT#: APMAB-1032LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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