Anti-PCNA Antibody (V2LY-0826-LY18) Immunohistochemistry Kit (V2LY-0826-LY149)

Name: Anti-PCNA Antibody (V2LY-0826-LY18) Immunohistochemistry Kit
SKU: V2LY-0826-LY149
Rating: 5 (1 reviews)

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Datasheet

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Datasheet Components References Q & As Review & reward Protocols Associated Products

Summary

Product Overview

This comprehensive, all-in-one kit is designed for the precise immunohistochemical (IHC) detection of target protein in tissue samples. Unlike standard detection kits, it includes a highly specific rabbit primary antibody bundled directly with our robust Polymer HRP detection system. The kit provides every essential reagent needed for the entire IHC workflow-from the initial antigen retrieval buffer to the final mounting media. By offering a pre-matched primary antibody with optimized buffers and secondary detection reagents, this kit eliminates the need for tedious protocol optimization and reagent sourcing, ensuring consistent, high-quality staining results.

Primary Antibody Cat.

V2LY-0826-LY18

Primary Antibody

Rabbit Anti-PCNA Recombinant Antibody (339)

Secondary Antibody Host Species

Goat

Secondary Antibody Species Specificity

Rabbit

Application

IHC-P

Sample Type

FFPE tissue

Test Size

50 Tests

Storage

Stored at 2-8°C. The kit is stable for 6 months from the date of receipt.

Key Features

Target-Specific Primary Antibody

Includes a highly validated rabbit primary antibody, ensuring reliable and specific target localization.

Complete End-to-End Solution

Contains all 11 essential components required for a full IHC workflow, including Tris-EDTA Antigen Retrieval Buffer, Blocking Buffer, Polymer HRP-Goat anti-Rabbit Secondary Antibody, DAB Chromogens, Counterstain, and Mounting Media.

Streamlined Workflow

Pre-optimized reagents work seamlessly with the included primary antibody, saving researchers significant time on antibody titration and protocol adjustments.

Sample Compatibility

Highly recommended and optimized for Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.

Kit Capacity

Provides sufficient reagents (50T) to perform 50 tests, depending on tissue section size.

More Infomation

Sub Cat	Reagents	Size
LV01	Antigen Retrieval Buffer (50× Tris-EDTA)	100 ml
LV02	Washing Buffer (30×)	120 ml
LV03	Blocking Buffer	5 ml
LV04	Quenching buffer (RTU)	5 ml
LV05	Primary Antibody Diluent (RTU)	100 ml
LV06R	HRP-Goat anti-Rabbit Secondary Antibody	5 ml
LV07	Chromogen Component A	0.2 ml
LV08	Chromogen Component B	4 ml
LV09	Signal Enhancer	5 ml
LV10	Counter Staining Reagent	5 ml
LV11	Mounting Media	5 ml

Bhardwaj, V. K., & Purohit, R. (2022). A lesson for the maestro of the replication fork: targeting the protein‐binding interface of proliferating cell nuclear antigen for anticancer therapy. Journal of Cellular Biochemistry, 123(6), 1091-1102.

Yu, S., Qiao, X., Song, X., Yang, Y., Zhang, D., Sun, W., ... & Song, L. (2022). The proliferating cell nuclear antigen (PCNA) is a potential proliferative marker in oyster Crassostrea gigas. Fish & Shellfish Immunology, 122, 306-315.

Liu, R., Sun, K., Wang, Y., Jiang, Y., Kang, J., & Ma, H. (2021). The effects of proliferating cell nuclear antigen and p53 in patients with oral squamous cell carcinoma: a systematic review and meta-analysis. Annals of translational medicine, 9(23).

Lim, S., Khoo, R., Peh, K. M., Teo, J., Chang, S. C., Ng, S., ... & Partridge, A. W. (2020). bioPROTACs as versatile modulators of intracellular therapeutic targets including proliferating cell nuclear antigen (PCNA). Proceedings of the National Academy of Sciences, 117(11), 5791-5800.

Cardano, M., Tribioli, C., & Prosperi, E. (2020). Targeting proliferating cell nuclear antigen (PCNA) as an effective strategy to inhibit tumor cell proliferation. Current cancer drug targets, 20(4), 240-252.

Mathews, M. B. (2020). The proliferating cell nuclear antigen, PCNA, a cell growth-regulated DNA replication factor. In Growth control during cell aging (pp. 89-120). CRC Press.

Ye, X., Ling, B., Xu, H., Li, G., Zhao, X., Xu, J., ... & Liu, L. (2020). Clinical significance of high expression of proliferating cell nuclear antigen in non-small cell lung cancer. Medicine, 99(16), e19755.

Lu, E. M. C., Ratnayake, J., & Rich, A. M. (2019). Assessment of proliferating cell nuclear antigen (PCNA) expression at the invading front of oral squamous cell carcinoma. BMC oral health, 19, 1-7.

Gonzalez-Magaña, A., De Opakua, A. I., Romano-Moreno, M., Murciano-Calles, J., Merino, N., Luque, I., ... & De Biasio, A. (2019). The p12 subunit of human polymerase δ uses an atypical PIP box for molecular recognition of proliferating cell nuclear antigen (PCNA). Journal of biological chemistry, 294(11), 3947-3956.

Peng, B., Ortega, J., Gu, L., Chang, Z., & Li, G. M. (2019). Phosphorylation of proliferating cell nuclear antigen promotes cancer progression by activating the ATM/Akt/GSK3β/Snail signaling pathway. Journal of Biological Chemistry, 294(17), 7037-7045.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant PCNA protein, His Tag (CAT#: V2LY-0526-LY5989)

Human Recombinant PCNA protein, His Tag-1 (CAT#: V2LY-0526-LY5990)

Chicken PCNA ELISA Kit (CAT#: V2LY-0626-LY628)

Rat Pcna ELISA Kit (CAT#: V2LY-0626-LY841)

Anti-PCNA Antibody (V2LY-0826-LY15) Immunohistochemistry Kit (CAT#: V2LY-0826-LY147)

Anti-PCNA Antibody (V2LY-0826-LY17) Immunohistochemistry Kit (CAT#: V2LY-0826-LY148)

Anti-PCNA Antibody (V2LY-0826-LY19) Immunohistochemistry Kit (CAT#: V2LY-0826-LY150)

Anti-PCNA Antibody (V2LY-0826-LY20) Immunohistochemistry Kit (CAT#: V2LY-0826-LY151)

Anti-PCNA Antibody (V2LY-0826-LY21) Immunohistochemistry Kit (CAT#: V2LY-0826-LY152)

Anti-PCNA Antibody (V2LY-0826-LY22) Immunohistochemistry Kit (CAT#: V2LY-0826-LY153)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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