Pig CD163 ELISA Kit (V2LY-0626-LY1003)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.025 μg/mL
Detection Range
0.05-10 μg/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Pig
Assay Type
Sandwich
Reactivity
Pig
Assay Time
1.5 h
Molecule Mass
120.5 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
CD163 Molecule
Function
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Biological Process
Acute-phase response Source: UniProtKB-KW
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Receptor-mediated endocytosis Source: Reactome
Cellular Location
Secreted; Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
Topology
Extracellular: 42-1050
Helical: 1051-1071
Cytoplasmic: 1072-1156
PTM
A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Phosphorylated.

Huang, C., Bernard, D., Zhu, J., Dash, R. C., Chu, A., Knupp, A., ... & Tang, Y. (2020). Small molecules block the interaction between porcine reproductive and respiratory syndrome virus and CD163 receptor and the infection of pig cells. Virology journal, 17(1), 1-11.

Zhang, Y., Zhang, K., Zheng, H., Liu, C., Jiang, Y., Du, N., ... & Gao, F. (2020). Development of a monoclonal antibody against porcine CD163 SRCR5 domain which partially blocks infection of PRRSV. Frontiers in Veterinary Science, 7, 923.

Kowalska, W. (2020). Expression of CD163 and HLA-DR molecules on the monocytes in chronic lymphocytic leukemia patients. Folia histochemica et cytobiologica, 58(1), 17-24.

Fujiwara, Y., Ohnishi, K., Horlad, H., Saito, Y., Shiraishi, D., Takeya, H., ... & Komohara, Y. (2020). CD163 deficiency facilitates lipopolysaccharide‐induced inflammatory responses and endotoxin shock in mice. Clinical & translational immunology, 9(9), e1162.

Nielsen, M. C., Hvidbjerg Gantzel, R., Clària, J., Trebicka, J., Møller, H. J., & Grønbæk, H. (2020). Macrophage activation markers, CD163 and CD206, in acute-on-chronic liver failure. Cells, 9(5), 1175.

Nunes, P. R., Romão-Veiga, M., Peraçoli, J. C., Costa, R. A. A., de Oliveira, L. G., Borges, V. T. M., & Peraçoli, M. T. (2019). Downregulation of CD163 in monocytes and its soluble form in the plasma is associated with a pro-inflammatory profile in pregnant women with preeclampsia. Immunologic research, 67(2), 194-201.

Liu, S., Zhang, C., Maimela, N. R., Yang, L., Zhang, Z., Ping, Y., ... & Zhang, Y. (2019). Molecular and clinical characterization of CD163 expression via large-scale analysis in glioma. Oncoimmunology, 8(7), e1601478.

Shiraishi, D., Fujiwara, Y., Horlad, H., Saito, Y., Iriki, T., Tsuboki, J., ... & Komohara, Y. (2018). CD163 is required for protumoral activation of macrophages in human and murine sarcoma. Cancer research, 78(12), 3255-3266.

Cheng, Z., Zhang, D., Gong, B., Wang, P., & Liu, F. (2017). CD163 as a novel target gene of STAT3 is a potential therapeutic target for gastric cancer. Oncotarget, 8(50), 87244.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human CD163 ELISA Kit (CAT#: V2LY-0626-LY24)

Mouse Cd163 ELISA Kit (CAT#: V2LY-0626-LY3011)

Human CD163 ELISA Kit (2) (CAT#: V2LY-0626-LY3827)

Related Products

Mouse Anti-CD163 Recombinant Antibody (GHI/61) (CAT#: CBMAB-C0676-CQ)

Mouse Anti-CD163 Recombinant Antibody (5C6-FAT) (CAT#: CBMAB-C7404-LY)

Mouse Anti-CD163 Recombinant Antibody (CBT3846) (CAT#: V2LY-0625-LY1341)

Mouse Anti-CD163 Recombinant Antibody (CBFYC-0444) (CAT#: CBMAB-C0490-FY)

Rabbit Anti-CD163 Recombinant Antibody (CBNH-018) (CAT#: CBMAB-R4343-CN)

Mouse Anti-CD163 Recombinant Antibody (CBFYC-0514) (CAT#: CBMAB-C0565-FY)

Mouse Anti-CD163 Recombinant Antibody (6D3.2F3) (CAT#: CBMAB-C8199-LY)

Human CD163 ELISA Kit (2) (CAT#: V2LY-0626-LY3827)

Human CD163 ELISA Kit (CAT#: V2LY-0626-LY24)

Mouse Anti-CD163 Recombinant Antibody (ED2) (CAT#: CBMAB-C10009-LY)

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For research use only. Not intended for any clinical use.

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