Rabbit Anti-5-Methylcytosine Recombinant Antibody (V2-7898) (CBMAB-2086CQ)

Name: Rabbit Anti-5-Methylcytosine Recombinant Antibody (V2-7898)
SKU: CBMAB-2086CQ
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-5-Methylcytosine Recombinant Antibody (D3S2Z) in IHC

Transverse sections of ascending aorta from WT or Eln-/- pups at P0.5 stained for 5-methylcytosine (5mC), SMA, and propidium iodide (PI, nuclei). Lu, lumen. Scale bar: 50 μm. ...View More

Dave, J. M., Chakraborty, R., Ntokou, A., Saito, J., Saddouk, F. Z., Feng, Z., ... & Greif, D. M. (2022). JAGGED1/NOTCH3 activation promotes aortic hypermuscularization and stenosis in elastin deficiency. The Journal of Clinical Investigation, 132(5).

Rabbit Anti-5-Methylcytosine Recombinant Antibody (D3S2Z) in IF

Representative images for immunofluorescent (IF) staining of 5mC (green) staining for blastocyst stage embryos obtained from IV In vivo developed embryos group, Single Single step medium, Seq Sequential medium. Mean ± S.D for the total signal intensity from each pixel of 5mC. ...View More

Uysal, F., Kahveci, S., Sukur, G., & Cinar, O. (2022). Embryo culture media differentially alter DNA methylating enzymes and global DNA methylation in embryos and oocytes. Journal of Molecular Histology, 53(1), 63-74.

Rabbit Anti-5-Methylcytosine Recombinant Antibody (D3S2Z) in IF

Effects of acupuncture on the expression levels of 5-mC in the PFC. Histological examinations of the PFC showing expression of 5-mC (green) with NeuN (red) after acupuncture administration (AP or CP) for 6 months (3 days/week) in a PSNL-induced neuropathic pain model. ...View More

Jang, J. H., Song, E. M., Do, Y. H., Ahn, S., Oh, J. Y., Hwang, T. Y., ... & Park, H. J. (2021). Acupuncture alleviates chronic pain and comorbid conditions in a mouse model of neuropathic pain: the involvement of DNA methylation in the prefrontal cortex. Pain, 162(2), 514.

Rabbit Anti-5-Methylcytosine Recombinant Antibody (D3S2Z) in WB

D2HGDH and L2HGDH roles on MYC-modulated DNA/RNA methylation and demethylase activity.
a) Left to right panels - MYC-induced modulation of 5hmC and 5mC levels in WT and D2HGDH or L2HGDH KO P493-6 cells, quantification of MYC-induced TET activity in WT and D2HGDH or L2HGDH KO P493-6 cells, and WBs of D2HGDH, L2HGDH, TET1, TET2, TET3 and MYC. b) Left to right panels - MYC-induced modulation of m6A levels in WT and D2HGDH or L2HGDH KO P493-6 cells, quantification of MYC-induced RNA demethylase activity in WT and D2HGDH or L2HGDH KO P493-6 cells, and WBs of FTO, ALKBH5, METL3, WTAP and METTL14 in WT and D2HGDH or L2HGDH KO P493-6 cells. c) Left to right panels - 5hmC and m6A levels in D2HGDH/L2HGDH WT or KO clones in DLBCL cell lines OCI-Ly1 and OCI-Ly8; WBs for D2HGDH and L2HGDH are shown at the top. The percentage of MYC-induced changes in a) and b) represent the difference between the measures in MYC OFF and ON cells (full data in Figure S3). Data shown are mean −/+ SD. In a) and b), TET1/ALKBH5 and TET3/FTO were detected in the same gel and share the β-actin loading control. ...View More

Qiu, Z., Lin, A. P., Jiang, S., Elkashef, S. M., Myers, J., Srikantan, S., ... & Aguiar, R. C. (2020). MYC regulation of D2HGDH and L2HGDH influences the epigenome and epitranscriptome. Cell chemical biology, 27(5), 538-550.

Rabbit Anti-5-Methylcytosine Recombinant Antibody (D3S2Z) in Dot blot

Dot-blot assay of 5-hmC and 5-mC in HNE1 and HONE1 cells with DNA concentration gradients. ...View More

Fan, J., Zhang, Y., Mu, J., He, X., Shao, B., Zhou, D., ... & Xiang, T. (2018). TET1 exerts its anti-tumor functions via demethylating DACT2 and SFRP2 to antagonize Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma cells. Clinical epigenetics, 10, 1-16.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

V2-7898

Application

DB, IF

Immunogen

5-methylcytidine

Host Species

Rabbit

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
DB	1:1,000
IF(ICC)	1:1,600

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES pH 7.5, 0.01% BSA, 50% Glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

5-Methylcytosine

Introduction

5-Methylcytosine is a methylated form of the DNA base cytosine that may be involved in the regulation of gene transcription. When cytosine is methylated, the DNA maintains the same sequence, but the expression of methylated genes can be altered (the study of this is part of the field of epigenetics). 5-Methylcytosine is incorporated in the nucleoside 5-methylcytidine

Alternative Names

5-Methylcytosine; 554-01-8; Cytosine, 5-methyl-; 2(1H)-Pyrimidinone, 4-amino-5-methyl-; 5-Methylcytosine (VAN); 5 Methylcytosine; 5-mC

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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