Rabbit Anti-5-Methylcytosine Recombinant Antibody (V2-7943) (CBMAB-2133CQ)

Name: Rabbit Anti-5-Methylcytosine Recombinant Antibody (V2-7943)
SKU: CBMAB-2133CQ
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-5-Methylcytosine Recombinant Antibody (RM231) in IF

Representative 5-methylcytosine distribution patterns in metaphase chromosomes of Secale.
Images for inverted DAPI, DAPI, and 5-methylcytosine are shown separately and merged. Secale cereale ssp. cereale, Secale vavilovii, Secale strictum ssp. kuprijanovii, Secale strictum ssp. africanum, Secale strictum ssp. strictum, Secale strictum, Secale cereale ssp. afghanicum, Secale cereale ssp. segetale. Scale bars represent 5 μm. ...View More

Kalinka, A., & Achrem, M. (2020). The distribution pattern of 5-methylcytosine in rye (Secale L.) chromosomes. Plos one, 15(10), e0240869.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

V2-7943

Application

DB, MD, ICC, IHC, ELISA

Immunogen

BSA-conjugated 5-methylcytosine

Host Species

Rabbit

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
DB	0.5-2 µg/ml
IF(ICC)	0.5-2 µg/ml
FC	0.5-2 µg/ml
IP	0.2-2 µg/ml
ELISA	0.1-3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2, 1% BSA, 50% glycerol

Preservative

0.09% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

5-Methylcytosine

Introduction

5-Methylcytosine is a methylated form of the DNA base cytosine that may be involved in the regulation of gene transcription. When cytosine is methylated, the DNA maintains the same sequence, but the expression of methylated genes can be altered (the study of this is part of the field of epigenetics). 5-Methylcytosine is incorporated in the nucleoside 5-methylcytidine

Alternative Names

5-Methylcytosine; 554-01-8; Cytosine, 5-methyl-; 2(1H)-Pyrimidinone, 4-amino-5-methyl-; 5-Methylcytosine (VAN); 5 Methylcytosine; 5-mC

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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