Human A2ML1 ELISA Kit (V2LY-0626-LY4331)

Name: Human A2ML1 ELISA Kit
SKU: V2LY-0626-LY4331
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

2.29 ng/mL

Detection Range

5-1000 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

161.1 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Alpha-2-Macroglobulin Like 1

Function

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity). Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases.

Biological Process

Regulation of endopeptidase activity

Cellular Location

Secreted

Involvement in disease

An inflammation of the middle ear resulting in earache, fever, hearing disturbance, and vertigo.

Brinkmann, J., Lissewski, C., Pinna, V., Vial, Y., Pantaleoni, F., Lepri, F., ... & Zenker, M. (2021). The clinical significance of A2ML1 variants in Noonan syndrome has to be reconsidered. European Journal of Human Genetics, 29(3), 524-527.

Kashiwagi, H., Ishimoto, H., Izumi, S. I., Seki, T., Kinami, R., Otomo, A., ... & Kametani, Y. (2020). Human PZP and common marmoset A2ML1 as pregnancy related proteins. Scientific reports, 10(1), 1-13.

Larson, E. D., Magno, J. P. M., Steritz, M. J., Llanes, E. G. D., Cardwell, J., Pedro, M., ... & Santos‐Cortez, R. L. P. (2019). A2ML1 and otitis media: novel variants, differential expression, and relevant pathways. Human mutation, 40(8), 1156-1171.

Cutiongco-de la Paz, E. M., Pedro, M., Yarza, T. K. L., Lagrana-Villagracia, S. M., Amoranto, A. J. P., Jover, E. J. M., ... & Santos-Cortez, R. L. P. (2019). Genetic counseling in an indigenous Filipino community with a high prevalence of A2ML1-related otitis media. Journal of community genetics, 10(1), 143-151.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-A2ML1 Recombinant Antibody (V2-448592) (CAT#: CBMAB-C1056-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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