Rat Csf2 ELISA Kit (V2LY-0626-LY869)

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Tested Data
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0014 ng/mL
Detection Range
0.003-0.9 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
16.6 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Colony Stimulating Factor 2
Function
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Biological Process
Cellular response to granulocyte macrophage colony-stimulating factor stimulus Source: CAFA
Cellular response to lipopolysaccharide Source: Ensembl
Cytokine-mediated signaling pathway Source: Reactome
Dendritic cell differentiation Source: DFLAT
Embryonic placenta development Source: Ensembl
Epithelial fluid transport Source: Ensembl
Histamine secretion Source: Ensembl
Immune response Source: InterPro
Macrophage activation Source: Ensembl
Macrophage differentiation Source: ARUK-UCL
MAPK cascade Source: Reactome
Monocyte differentiation Source: Ensembl
Myeloid cell differentiation Source: GO_Central
Myeloid dendritic cell differentiation Source: UniProtKB
Negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: UniProtKB
Neutrophil differentiation Source: Ensembl
Positive regulation of cell population proliferation Source: BHF-UCL
Positive regulation of gene expression Source: BHF-UCL
Positive regulation of interleukin-23 production Source: BHF-UCL
Positive regulation of macrophage derived foam cell differentiation Source: BHF-UCL
Positive regulation of podosome assembly Source: BHF-UCL
Positive regulation of tyrosine phosphorylation of STAT protein Source: BHF-UCL
Regulation of circadian sleep/wake cycle, sleep Source: Ensembl
Regulation of myeloid cell differentiation Source: Reactome
Response to fluid shear stress Source: Ensembl
Response to silicon dioxide Source: Ensembl
Cellular Location
Secreted

Xiao, Y., Uh, K., Negrón-Pérez, V. M., Haines, H., Lee, K., & Hansen, P. J. (2021). Regulation of gene expression in the bovine blastocyst by colony-stimulating factor 2 is disrupted by CRISPR/Cas9-mediated deletion of CSF2RA. Biology of Reproduction, 104(5), 995-1007.

Estrada-Cortés, E., Jannaman, E. A., Block, J., Amaral, T. F., & Hansen, P. J. (2021). Programming of postnatal phenotype caused by exposure of cultured embryos from Brahman cattle to colony-stimulating factor 2 and serum. Journal of Animal Science, 99(8), skab180.

Sinkey, R. G., Guzeloglu-Kayisli, O., Arlier, S., Guo, X., Semerci, N., Moore, R., ... & Lockwood, C. J. (2020). Thrombin-induced decidual colony-stimulating factor-2 promotes abruption-related preterm birth by weakening fetal membranes. The American journal of pathology, 190(2), 388-399.

Sosa, F., Block, J., Xiao, Y., & Hansen, P. J. (2020). Determinants of survival of the bovine blastocyst to cryopreservation stress: treatment with colony stimulating factor 2 during the morula-to-blastocyst transition and embryo sex. CABI agriculture and bioscience, 1(1), 1-10.

Sielska, M., Przanowski, P., Pasierbińska, M., Wojnicki, K., Poleszak, K., Wojtas, B., ... & Kaminska, B. (2020). Tumour-derived CSF2/granulocyte macrophage colony stimulating factor controls myeloid cell accumulation and progression of gliomas. British journal of cancer, 123(3), 438-448.

Sharkey, D. J., Glynn, D. J., Schjenken, J. E., Tremellen, K. P., & Robertson, S. A. (2018). Interferon-gamma inhibits seminal plasma induction of colony-stimulating factor 2 in mouse and human reproductive tract epithelial cells. Biology of reproduction, 99(3), 514-526.

Tríbulo, P., Bernal Ballesteros, B. H., Ruiz, A., Tríbulo, A., Tríbulo, R. J., Tríbulo, H. E., ... & Hansen, P. J. (2017). Consequences of exposure of embryos produced in vitro in a serum-containing medium to dickkopf-related protein 1 and colony stimulating factor 2 on blastocyst yield, pregnancy rate, and birth weight. Journal of animal science, 95(10), 4407-4412.

Siqueira, L. G., Tribulo, P., Chen, Z., Denicol, A. C., Ortega, M. S., Negrón-Pérez, V. M., ... & Hansen, P. J. (2017). Colony-stimulating factor 2 acts from days 5 to 7 of development to modify programming of the bovine conceptus at day 86 of gestation. Biology of Reproduction, 96(4), 743-757.

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For research use only. Not intended for any clinical use.

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