Human ADAMTS7 ELISA Kit (V2LY-0626-LY1418)

Name: Human ADAMTS7 ELISA Kit
SKU: V2LY-0626-LY1418
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.65 ng/mL

Detection Range

1-400 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

184.1 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

ADAM Metallopeptidase With Thrombospondin Type 1 Motif 7

Function

Metalloprotease that may play a role in the degradation of COMP.

Biological Process

Cellular response to BMP stimulus
Cellular response to interleukin-1
Cellular response to tumor necrosis factor
Extracellular matrix organization
Negative regulation of chondrocyte differentiation
Proteolysis involved in cellular protein catabolic process

Cellular Location

Extracellular matrix. Also found associated with the external cell surface.

PTM

N-glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs. N- and C-glycosylations can also facilitate secretion. O-glycosylated proteoglycan. Contains chondroitin sulfate.
May be cleaved by a furin endopeptidase (By similarity). The precursor is sequentially processed.

Mizoguchi, T., MacDonald, B. T., Bhandary, B., Popp, N. R., Laprise, D., Arduini, A., ... & Ellinor, P. T. (2021). Coronary Disease Association with ADAMTS7 is due to Protease Activity. Circulation Research.

Taştemur, M., Beysel, S., Hepşen, S., Öztekin, S., Çakal, E., Akdağ, İ., & Yıldız, M. (2021). Investigating ADAMTS7 and ADAMTS12 levels in prediabetic and Type 2 diabetic patients. Biomarkers in Medicine, (0).

Ren, W., Liang, L., Li, Y., Wei, F. Y., Mu, N., Zhang, L., ... & Li, H. (2020). Upregulation of miR‑423 improves autologous vein graft restenosis via targeting ADAMTS‑7. International journal of molecular medicine, 45(2), 532-542.

Wang, C., Chen, Y., Xiang, H., Wu, X., Tang, Q., Ma, X., & Zhang, L. (2020). ADAMTS7 degrades Comp to fuel BMP2‐dependent osteogenic differentiation and ameliorate oncogenic potential in osteosarcomas. FEBS Open bio, 10(9), 1856-1867.

Mead, T. J., McCulloch, D. R., Ho, J. C., Du, Y., Adams, S. M., Birk, D. E., & Apte, S. S. (2018). The metalloproteinase-proteoglycans ADAMTS7 and ADAMTS12 provide an innate, tendon-specific protective mechanism against heterotopic ossification. JCI insight, 3(7).

Bayoglu, B., Arslan, C., Tel, C., Ulutin, T., Dirican, A., Deser, S. B., & Cengiz, M. (2018). Genetic variants rs1994016 and rs3825807 in ADAMTS7 affect its mRNA expression in atherosclerotic occlusive peripheral arterial disease. Journal of clinical laboratory analysis, 32(1), e22174.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-ADAMTS7 Recombinant Antibody (V2-448598) (CAT#: CBMAB-C1066-CN)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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