Rat Il3 ELISA Kit (V2LY-0626-LY436)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.00047 ng/mL
Detection Range
0.001-0.4 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
18.6 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Interleukin 3
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Biological Process
Cell-cell signalingManual Assertion Based On ExperimentTAS:ProtInc
Embryonic hemopoiesisManual Assertion Based On ExperimentIDA:DFLAT
Immune responseIEA:InterPro
Nervous system developmentManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of peptidyl-tyrosine phosphorylationISS:UniProtKB
Positive regulation of tyrosine phosphorylation of STAT proteinManual Assertion Based On ExperimentIDA:BHF-UCL
Cellular Location
Secreted

Wang, H., Tumes, D. J., Hercus, T. R., Yip, K. H., Aloe, C., Vlahos, R., ... & Bozinovski, S. (2022). Blocking the human common beta subunit of the GM-CSF, IL-5 and IL-3 receptors markedly reduces hyperinflammation in ARDS models. Cell Death & Disease, 13(2), 137.

Rani, L., Kumar, A., Karhade, J., Pandey, G., Guha, A., Mishra, G. C., & Wani, M. R. (2022). IL‐3 regulates the differentiation of pathogenic Th17 cells. European Journal of Immunology, 52(11), 1842-1858.

Varricchi, G., Poto, R., Marone, G., & Schroeder, J. T. (2021, April). IL-3 in the development and function of basophils. In Seminars in immunology (Vol. 54, p. 101510). Academic Press.

Aldoss, I., Clark, M., Song, J. Y., & Pullarkat, V. (2020). Targeting the alpha subunit of IL-3 receptor (CD123) in patients with acute leukemia. Human vaccines & immunotherapeutics, 16(10), 2341-2348.

Tong, Y., Lear, T. B., Evankovich, J., Chen, Y., Londino, J. D., Myerburg, M. M., ... & Chen, B. B. (2020). The RNFT2/IL-3Rα axis regulates IL-3 signaling and innate immunity. JCI insight, 5(3).

Kumar, A., Rani, L., Mhaske, S. T., Pote, S. T., Behera, S., Mishra, G. C., & Wani, M. R. (2020). IL-3 receptor expression on activated human Th cells is regulated by IL-4, and IL-3 synergizes with IL-4 to enhance Th2 cell differentiation. The Journal of Immunology, 204(4), 819-831.

Dougan, M., Dranoff, G., & Dougan, S. K. (2019). GM-CSF, IL-3, and IL-5 family of cytokines: regulators of inflammation. Immunity, 50(4), 796-811.

Borriello, F., Galdiero, M. R., Varricchi, G., Loffredo, S., Spadaro, G., & Marone, G. (2019). Innate immune modulation by GM-CSF and IL-3 in health and disease. International journal of molecular sciences, 20(4), 834.

Zaidi, N., Quezada, S. A., Kuroiwa, J. M., Zhang, L., Jaffee, E. M., Steinman, R. M., & Wang, B. (2019). Anti–CTLA‐4 synergizes with dendritic cell–targeted vaccine to promote IL‐3–dependent CD4+ effector T cell infiltration into murine pancreatic tumors. Annals of the New York Academy of Sciences, 1445(1), 62-73.

Broughton, S. E., Hercus, T. R., Nero, T. L., Kan, W. L., Barry, E. F., Dottore, M., ... & Parker, M. W. (2018). A dual role for the N-terminal domain of the IL-3 receptor in cell signalling. Nature communications, 9(1), 386.

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For research use only. Not intended for any clinical use.

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