Rat Nrf1 ELISA Kit (V2LY-0626-LY1335)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.11 ng/mL
Detection Range
0.2-60 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
5.3 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
nuclear respiratory factor 1
Function
Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
Biological Process
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:NTNU_SB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
PTM
Phosphorylation enhances DNA binding.

Perez, C. M., Gong, Z., Yoo, C., Roy, D., Deoraj, A., & Felty, Q. (2024). Inhibitor of DNA Binding Protein 3 (ID3) and Nuclear Respiratory Factor 1 (NRF1) Mediated Transcriptional Gene Signatures Are Associated with the Severity of Cerebral Amyloid Angiopathy. Molecular Neurobiology, 61(2), 835-882.

Lee, Y. H., Wu, R. C., Mai, H. C., Huang, W. L., Wu, C. H., Yang, Y. L., ... & Lin, V. C. (2023). The regulatory role of nuclear Respiratory factor 1 in bladder cancer cells. Anticancer Research, 43(4), 1521-1531.

Wang, D., Wan, B., Zhang, X., Sun, P., Lu, S., Liu, C., & Zhu, L. (2022). Nuclear respiratory factor 1 promotes the growth of liver hepatocellular carcinoma cells via E2F1 transcriptional activation. BMC gastroenterology, 22(1), 198.

Wu, C. H., Hsieh, P. F., Lee, Y. H., Kuo, W. W. T., Wu, R. C. Y., Lin, Y. Y., ... & Lin, V. C. (2022). Nuclear respiratory factor 1 overexpression inhibits proliferation and migration of PC3 prostate cancer cells. Cancer Genomics & Proteomics, 19(5), 614-623.

Li, H., Niu, N., Yang, J., Dong, F., Zhang, T., Li, S., & Zhao, W. (2021). Nuclear respiratory factor 1 protects H9C2 cells against hypoxia‐induced apoptosis via the death receptor pathway and mitochondrial pathway. Cell Biology International, 45(8), 1784-1796.

Lu, Y., Ding, W., Wang, B., Wang, L., Kan, H., Wang, X., ... & Zhu, L. (2020). Positive regulation of human PINK1 and Parkin gene expression by nuclear respiratory factor 1. Mitochondrion, 51, 22-29.

Alam, C., Hoque, M. T., Sangha, V., & Bendayan, R. (2020). Nuclear respiratory factor 1 (NRF‐1) upregulates the expression and function of reduced folate carrier (RFC) at the blood‐brain barrier. The FASEB Journal, 34(8), 10516-10530.

Bhawe, K., Felty, Q., Yoo, C., Ehtesham, N. Z., Hasnain, S. E., Singh, V. P., ... & Roy, D. (2020). Nuclear respiratory factor 1 (NRF1) transcriptional activity-driven gene signature association with severity of astrocytoma and poor prognosis of glioblastoma. Molecular neurobiology, 57, 3827-3845.

Ayeleso, A. O., Adepoju, A. E., & Mukwevho, E. (2020). Nuclear respiratory factor-1 (NRF-1), a versatile therapeutic target: influence of plant metabolites. African journal of biomedical research, 23(1), 1-8.

Niu, N., Li, Z., Zhu, M., Sun, H., Yang, J., Xu, S., ... & Song, R. (2019). Effects of nuclear respiratory factor‑1 on apoptosis and mitochondrial dysfunction induced by cobalt chloride in H9C2 cells. Molecular medicine reports, 19(3), 2153-2163.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Nrf1 ELISA Kit (CAT#: V2LY-0626-LY1562)

Human NRF1 ELISA Kit (CAT#: V2LY-0626-LY1954)

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Human NRF1 ELISA Kit (CAT#: V2LY-0626-LY1954)

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For research use only. Not intended for any clinical use.

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