Rat Apom ELISA Kit (V2LY-0626-LY1035)

Name: Rat Apom ELISA Kit
SKU: V2LY-0626-LY1035
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.27 ng/mL

Detection Range

0.5-200 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Rat

Assay Type

Sandwich

Reactivity

Rat

Assay Time

1.5 h

Molecule Mass

21.5 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Apolipoprotein M

Function

Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.

Biological Process

Cholesterol efflux Source: BHF-UCL
Cholesterol homeostasis Source: BHF-UCL
High-density lipoprotein particle assembly Source: BHF-UCL
High-density lipoprotein particle clearance Source: BHF-UCL
High-density lipoprotein particle remodeling Source: BHF-UCL
Lipoprotein metabolic process Source: Ensembl
Negative regulation of plasma lipoprotein oxidation Source: BHF-UCL
Response to glucose Source: Ensembl
Retinoid metabolic process Source: Reactome
Reverse cholesterol transport Source: BHF-UCL

Cellular Location

Secreted. Present in high density lipoprotein (HDL) and to a lesser extent in triglyceride-rich lipoproteins (TGRLP) and low density lipoproteins (LDL).

Hajny, S., Christoffersen, M., Dalila, N., Nielsen, L. B., Tybjærg-Hansen, A., & Christoffersen, C. (2020). Apolipoprotein M and risk of type 2 diabetes. The Journal of Clinical Endocrinology & Metabolism, 105(9), 3046-3057.

Kobayashi, T., Kurano, M., Nanya, M., Shimizu, T., Ohkawa, R., Tozuka, M., & Yatomi, Y. (2020). Glycation of HDL polymerizes apolipoprotein M and attenuates its capacity to bind to sphingosine 1-phosphate. Journal of Atherosclerosis and Thrombosis, 55699.

Wang, M., Luo, G. H., Liu, H., Zhang, Y. P., Wang, B., Di, D. M., ... & Xu, N. (2019). Apolipoprotein M induces inhibition of inflammatory responses via the S1PR1 and DHCR24 pathways. Molecular medicine reports, 19(2), 1272-1283.

Sramkova, V., Berend, S., Siklova, M., Caspar-Bauguil, S., Carayol, J., Bonnel, S., ... & Viguerie, N. (2019). Apolipoprotein M: a novel adipokine decreasing with obesity and upregulated by calorie restriction. The American journal of clinical nutrition, 109(6), 1499-1510.

Liu, Y., & Tie, L. (2019). Apolipoprotein M and sphingosine-1-phosphate complex alleviates TNF-α-induced endothelial cell injury and inflammation through PI3K/AKT signaling pathway. BMC cardiovascular disorders, 19(1), 1-9.

Yang, L., Li, T., Zhao, S., & Zhang, S. (2019). Expression of apolipoprotein M and its association with adiponectin in an obese mouse model. Experimental and Therapeutic Medicine, 18(3), 1685-1692.

Yu, M., Pan, L., Sang, C., Mu, Q., Zheng, L., Luo, G., & Xu, N. (2019). Apolipoprotein M could inhibit growth and metastasis of SMMC7721 cells via vitamin D receptor signaling. Cancer management and research, 11, 3691.

Kurano, M., Tsuneyama, K., Morimoto, Y., Nishikawa, M., & Yatomi, Y. (2019). Apolipoprotein M suppresses the phenotypes of IgA nephropathy in hyper‐IgA mice. The FASEB Journal, 33(4), 5181-5195.

Croyal, M., Billon-Crossouard, S., Goulitquer, S., Aguesse, A., León, L., Fall, F., ... & Krempf, M. (2018). Stable isotope kinetic study of ApoM (apolipoprotein M). Arteriosclerosis, thrombosis, and vascular biology, 38(1), 255-261.

Bosteen, M. H., Svarrer, E. M. M., Bisgaard, L. S., Martinussen, T., Madsen, M., Nielsen, L. B., ... & Pedersen, T. X. (2017). Effects of apolipoprotein M in uremic atherosclerosis. Atherosclerosis, 265, 93-101.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant APOM protein, His Tag (CAT#: V2LY-0526-LY2153)

Human Recombinant APOM protein, hFc Tag (CAT#: V2LY-0526-LY2154)

Human Recombinant APOM protein, His Tag-1 (CAT#: V2LY-0526-LY2155)

Human APOM ELISA Kit (CAT#: V2LY-0626-LY3648)

Mouse Apom ELISA Kit (CAT#: V2LY-0626-LY872)

APOM Matched Antibody Pair (097) (CAT#: APMAB-097LY)

Antibody Pairs

APOM Matched Antibody Pair (097) (CAT#: APMAB-097LY)

Related Products

Human Recombinant APOM protein, His Tag (CAT#: V2LY-0526-LY2153)

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Human Recombinant APOM protein, hFc Tag (CAT#: V2LY-0526-LY2154)

Mouse Anti-APOM Recombinant Antibody (4C7) (CAT#: CBMAB-A3275-YC)

Mouse Anti-ApoM Recombinant Antibody (CBT3373) (CAT#: V2LY-0625-LY2120)

Mouse Anti-APOM Recombinant Antibody (2E5) (CAT#: CBMAB-A3273-YC)

Mouse Apom ELISA Kit (CAT#: V2LY-0626-LY872)

Mouse Anti-ApoM Recombinant Antibody (CBT3641) (CAT#: V2LY-0625-LY2121)

Mouse Anti-APOM Recombinant Antibody (3C7) (CAT#: CBMAB-A0429-LY)

Mouse Anti-APOM Recombinant Antibody (CBT175) (CAT#: V2LY-0625-LY3738)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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