Rat Pcp4 ELISA Kit (V2LY-0626-LY1877)

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Tested Data
Datasheet
SDS
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.11 ng/mL
Detection Range
0.19-12 ng/mL
Sample Type
Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
6.8 kDa
Components
  • Standard Solution: 0.5ml x1
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard Diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop Solution: 6ml x1
  • Substrate Solution A: 6ml x1
  • Substrate Solution B: 6ml x1
  • Wash Buffer Concentrate (25x): 20ml x1
  • Biotinylated Antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Purkinje cell protein 4
Function
Functions as a modulator of calcium-binding by calmodulin. Thereby, regulates calmodulin activity and the different processes it controls (PubMed:19106096, PubMed:23204517, PubMed:27876793).
For instance, may play a role in neuronal differentiation through activation of calmodulin-dependent kinase signaling pathways (PubMed:21491429).
Biological Process
Calmodulin dependent kinase signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of neuron differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
cytoplasm
cytosol
nucleus
protein-containing complex

Hu, D., Dong, X., Wang, Q., Liu, M., Luo, S., Meng, Z., ... & Song, W. (2023). PCP4 Promotes Alzheimer’s Disease Pathogenesis by Affecting Amyloid-β Protein Precursor Processing. Journal of Alzheimer's Disease, (Preprint), 1-14.

Kitazono, I., Hamada, T., Yoshimura, T., Kirishima, M., Yokoyama, S., Akahane, T., & Tanimoto, A. (2020). PCP4/PEP19 downregulates neurite outgrowth via transcriptional regulation of Ascl1 and NeuroD1 expression in human neuroblastoma M17 cells. Laboratory Investigation, 100(12), 1551-1563.

He, L., Lee, G. T., Zhou, H., Buhimschi, I. A., Buhimschi, C. S., Weiner, C. P., & Mason, C. W. (2019). Expression, regulation, and function of the calmodulin accessory protein PCP4/PEP-19 in myometrium. Reproductive Sciences, 26(12), 1650-1660.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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