Rat Recombinant AK1 protein, His Tag (V2LY-0526-LY9155)

Name: Rat Recombinant AK1 protein, His Tag
SKU: V2LY-0526-LY9155
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant AK1 protein, His Tag consist of Amino Acid: 1-194 and predicts a molecular mass of 23.8 kDa.

Molecule Mass

23.8 kDa

Sequence

Amino Acid: 1-194

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, Urea, β-Mercaptoethanol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

adenylate kinase 1

Function

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.

Biological Process

ADP biosynthetic process Source: UniProtKB-UniRule
AMP metabolic process Source: UniProtKB-UniRule
ATP metabolic process Source: UniProtKB-UniRule
Nucleobase-containing small molecule interconversion Source: Reactome
Nucleoside diphosphate phosphorylation Source: UniProtKB
Nucleoside triphosphate biosynthetic process Source: UniProtKB

Cellular Location

Cytoplasm

Involvement in disease

Hemolytic anemia due to adenylate kinase deficiency (HAAKD): A disease characterized by hemolytic anemia and undetectable erythrocyte adenylate kinase activity.

Zervou, S., McAndrew, D. J., Whittington, H. J., Lake, H. A., Park, K. C., Cha, K. M., ... & Lygate, C. A. (2021). Subtle role for adenylate kinase 1 in maintaining normal basal contractile function and metabolism in the murine heart. Frontiers in physiology, 12, 454.

Tang, C. L., Zhang, R. H., Li, R., Li, X. R., Pan, Q., Li, L., & Xiao, J. L. (2021). EFFECT OF ADENYLATE KINASE 1 ON THE GROWTH AND DEVELOPMENT OF SCHISTOSOMA JAPONICUM SCHISTOSOMULUM. The Journal of Parasitology, 107(3), 472-480.

Xie, M., Zhang, G., Zhang, H., Chen, F., Chen, Y., Zhuang, Y., ... & Chen, Z. (2020). Adenylate kinase 1 deficiency disrupts mouse sperm motility under conditions of energy stress. Biology of Reproduction, 103(5), 1121-1131.

Gao, Y. R., Chen, L., Li, M. C., Li, Y. H., Lei, L., Wu, Q., ... & Li, L. (2020). Role of adenylate kinase 1 in the integument development of Schistosoma japonicum schistosomula. Acta tropica, 207, 105467.

Jan, Y. H., Lai, T. C., Yang, C. J., Huang, M. S., & Hsiao, M. (2019). A co-expressed gene status of adenylate kinase 1/4 reveals prognostic gene signature associated with prognosis and sensitivity to EGFR targeted therapy in lung adenocarcinoma. Scientific reports, 9(1), 1-11.

Gao, Y., Zhou, X., Wang, H., Liu, R., Ye, Q., Zhao, Q., ... & Dong, H. (2017). Immunization with recombinant schistosome adenylate kinase 1 partially protects mice against Schistosoma japonicum infection. Parasitology research, 116(6), 1665-1674.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant AK1 protein, His Tag (CAT#: V2LY-0526-LY1949)

AK1 Matched Antibody Pair (044) (CAT#: APMAB-044LY)

AK1 Matched Antibody Pair (045) (CAT#: APMAB-045LY)

AK1 Matched Antibody Pair (046) (CAT#: APMAB-046LY)

AK1 Matched Antibody Pair (047) (CAT#: APMAB-047LY)

AK1 Matched Antibody Pair (048) (CAT#: APMAB-048LY)

AK1 Matched Antibody Pair (049) (CAT#: APMAB-049LY)

AK1 Matched Antibody Pair (050) (CAT#: APMAB-050LY)

Antibody Pairs

AK1 Matched Antibody Pair (044) (CAT#: APMAB-044LY)

AK1 Matched Antibody Pair (045) (CAT#: APMAB-045LY)

AK1 Matched Antibody Pair (046) (CAT#: APMAB-046LY)

AK1 Matched Antibody Pair (047) (CAT#: APMAB-047LY)

AK1 Matched Antibody Pair (048) (CAT#: APMAB-048LY)

AK1 Matched Antibody Pair (049) (CAT#: APMAB-049LY)

AK1 Matched Antibody Pair (050) (CAT#: APMAB-050LY)

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Mouse Anti-AK1 Recombinant Antibody (V2-180373) (CAT#: CBMAB-A1845-YC)

AK1 Matched Antibody Pair (045) (CAT#: APMAB-045LY)

AK1 Matched Antibody Pair (046) (CAT#: APMAB-046LY)

AK1 Matched Antibody Pair (049) (CAT#: APMAB-049LY)

Mouse Anti-AK1 Recombinant Antibody (V2-180378) (CAT#: CBMAB-A1850-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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