Rat Recombinant AXL protein, ECD, His Tag (V2LY-0526-LY9166)

Name: Rat Recombinant AXL protein, ECD, His Tag
SKU: V2LY-0526-LY9166
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant AXL protein, ECD, His Tag consist of Amino Acid: 1-434 and predicts a molecular mass of 46.72 kDa.

Molecule Mass

46.72 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 1-434

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.>95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, DTT

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

AXL Receptor Tyrosine Kinase

Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.
(Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.
(Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.

Biological Process

Animal organ regeneration Source: Ensembl
Blood vessel remodeling Source: Ensembl
Cell maturation Source: UniProtKB
Cell migration Source: GO_Central
Cellular response to extracellular stimulus Source: Ensembl
Cellular response to hydrogen peroxide Source: Ensembl
Cellular response to interferon-alpha Source: UniProtKB
Cellular response to lipopolysaccharide Source: UniProtKB
Dendritic cell differentiation Source: UniProtKB
Erythrocyte homeostasis Source: Ensembl
Forebrain cell migration Source: Ensembl
Inflammatory response Source: Ensembl
Innate immune response Source: UniProtKB-KW
Multicellular organism development Source: GO_Central
Natural killer cell differentiation Source: GO_Central
Negative regulation of apoptotic process Source: GO_Central
Negative regulation of dendritic cell apoptotic process Source: UniProtKB
Negative regulation of interferon-gamma production Source: UniProtKB
Negative regulation of lymphocyte activation Source: Ensembl
Negative regulation of neuron apoptotic process Source: Ensembl
Negative regulation of tumor necrosis factor production Source: Ensembl
Nervous system development Source: GO_Central
Neuron migration Source: Ensembl
Neutrophil clearance Source: Ensembl
Ovulation cycle Source: Ensembl
Phagocytosis Source: UniProtKB
Platelet activation Source: GO_Central
Positive regulation of cytokine-mediated signaling pathway Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Positive regulation of natural killer cell differentiation Source: UniProtKB
Positive regulation of pinocytosis Source: CACAO
Positive regulation of protein kinase B signaling Source: GO_Central
Positive regulation of viral life cycle Source: FlyBase
Protein kinase B signaling Source: Ensembl
Secretion by cell Source: Ensembl
Signal transduction Source: ProtInc
Spermatogenesis Source: Ensembl
Substrate adhesion-dependent cell spreading Source: Ensembl
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Vagina development Source: Ensembl
Vascular endothelial growth factor receptor signaling pathway Source: Reactome
Viral entry into host cell Source: CACAO

Cellular Location

Cell membrane

Topology

Extracellular: 26-451 aa
Helical: 452-472 aa
Cytoplasmic: 473-894 aa

PTM

Monoubiquitinated upon GAS6-binding. A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion.
Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.

DeBerge, M., Glinton, K., Subramanian, M., Wilsbacher, L. D., Rothlin, C. V., Tabas, I., & Thorp, E. B. (2021). Macrophage AXL receptor tyrosine kinase inflames the heart after reperfused myocardial infarction. The Journal of clinical investigation, 131(6).

Brenig, R., Pop, O. T., Triantafyllou, E., Geng, A., Singanayagam, A., Perez-Shibayama, C., ... & Bernsmeier, C. (2020). Expression of AXL receptor tyrosine kinase relates to monocyte dysfunction and severity of cirrhosis. Life science alliance, 3(1).

Namiki, K., Wongsirisin, P., Yokoyama, S., Sato, M., Rawangkan, A., Sakai, R., ... & Suganuma, M. (2020). (−)-Epigallocatechin gallate inhibits stemness and tumourigenicity stimulated by AXL receptor tyrosine kinase in human lung cancer cells. Scientific reports, 10(1), 1-11.

Lauter, M., Weber, A., & Torka, R. (2019). Targeting of the AXL receptor tyrosine kinase by small molecule inhibitor leads to AXL cell surface accumulation by impairing the ubiquitin-dependent receptor degradation. Cell Communication and Signaling, 17(1), 1-14.

Yan, S., Vandewalle, N., De Beule, N., Faict, S., Maes, K., De Bruyne, E., ... & De Veirman, K. (2019). AXL receptor tyrosine kinase as a therapeutic target in hematological malignancies: focus on multiple myeloma. Cancers, 11(11), 1727.

Zhang, G., Wang, M., Zhao, H., & Cui, W. (2018). Function of Axl receptor tyrosine kinase in non-small cell lung cancer. Oncology letters, 15(3), 2726-2734.

Badi, I., Mancinelli, L., Polizzotto, A., Ferri, D., Zeni, F., Burba, I., ... & Raucci, A. (2018). miR-34a promotes vascular smooth muscle cell calcification by downregulating SIRT1 (Sirtuin 1) and Axl (AXL Receptor Tyrosine Kinase). Arteriosclerosis, thrombosis, and vascular biology, 38(9), 2079-2090.

Zhen, Y., Lee, I. J., Finkelman, F. D., & Shao, W. H. (2018). Targeted inhibition of Axl receptor tyrosine kinase ameliorates anti-GBM-induced lupus-like nephritis. Journal of autoimmunity, 93, 37-44.

Kanlikilicer, P., Ozpolat, B., Aslan, B., Bayraktar, R., Gurbuz, N., Rodriguez-Aguayo, C., ... & Lopez-Berestein, G. (2017). Therapeutic targeting of AXL receptor tyrosine kinase inhibits tumor growth and intraperitoneal metastasis in ovarian cancer models. Molecular Therapy-Nucleic Acids, 9, 251-262.

Aguilera, T. A., & Giaccia, A. J. (2017). Molecular pathways: oncologic pathways and their role in T-cell exclusion and immune evasion—a new role for the AXL receptor tyrosine kinase. Clinical Cancer Research, 23(12), 2928-2933.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant AXL protein, ECD, His Tag (CAT#: V2LY-0526-LY2222)

Human Recombinant AXL protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY2223)

Human Recombinant AXL protein, ECD, Fc Tag (CAT#: V2LY-0526-LY2224)

Human Recombinant AXL, Active protein, GST Tag (CAT#: V2LY-0526-LY2225)

Human Recombinant AXL , Active protein, His Tag (CAT#: V2LY-0526-LY2226)

Human Recombinant AXL (R499C), Active protein, GST Tag (CAT#: V2LY-0526-LY2227)

Mouse Recombinant AXL protein, His & hFc Tag (CAT#: V2LY-0526-LY7832)

Mouse Recombinant AXL protein, His Tag (CAT#: V2LY-0526-LY7833)

Mouse Recombinant AXL protein, His Tag-1 (CAT#: V2LY-0526-LY7834)

Human AXL ELISA Kit (CAT#: V2LY-0626-LY3733)

Antibody Pairs

AXL Matched Antibody Pair (119) (CAT#: APMAB-119LY)

Related Products

Human Recombinant AXL , Active protein, His Tag (CAT#: V2LY-0526-LY2226)

Human Recombinant AXL, Active protein, GST Tag (CAT#: V2LY-0526-LY2225)

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Human Anti-AXL Recombinant Antibody (
6G10) (CAT#: V2LY-0725-LY1188)

Rabbit Anti-Axl Recombinant Antibody (C2B12) (CAT#: CBMAB-A4238-YC)

Human Recombinant AXL (R499C), Active protein, GST Tag (CAT#: V2LY-0526-LY2227)

Human AXL ELISA Kit (CAT#: V2LY-0626-LY3733)

Mouse Recombinant AXL protein, His Tag (CAT#: V2LY-0526-LY7833)

Human Recombinant AXL protein, ECD, Fc Tag (CAT#: V2LY-0526-LY2224)

AXL Matched Antibody Pair (119) (CAT#: APMAB-119LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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