Rat Recombinant CD99 protein, hFc Tag (V2LY-0526-LY9278)

Name: Rat Recombinant CD99 protein, hFc Tag
SKU: V2LY-0526-LY9278
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

hFc Tag

Protein Construction

This product is Rat Recombinant CD99 protein, hFc Tag consist of Amino Acid: 1-127 and predicts a molecular mass of 37.6 kDa.

Molecule Mass

37.6 kDa

Sequence

Amino Acid: 1-127

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD99 Molecule (Xg Blood Group)

Function

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).

Biological Process

Homotypic cell-cell adhesion Source: GO_Central
Positive regulation of neutrophil extravasation Source: GO_Central
Regulation of immune response Source: Reactome
T cell extravasation Source: GO_Central

Cellular Location

Membrane

Topology

Extracellular: 23-122
Helical: 123-147
Cytoplasmic: 148-185

PTM

Extensively O-glycosylated.

Siwy, J., Wendt, R., Albalat, A., He, T., Mischak, H., Mullen, W., ... & Beige, J. (2021). CD99 and polymeric immunoglobulin receptor peptides deregulation in critical COVID‐19: A potential link to molecular pathophysiology?. Proteomics, 21(20), 2100133.

Mattisson, J., Danielsson, M., Hammond, M., Davies, H., Gallant, C. J., Nordlund, J., ... & Forsberg, L. A. (2021). Leukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD99. Scientific Reports, 11(1), 1-9.

Takheaw, N., Sittithumcharee, G., Kariya, R., Kasinrerk, W., & Okada, S. (2021). Anti-human CD99 antibody exerts potent antitumor effects in mantle cell lymphoma. Cancer Immunology, Immunotherapy, 70(6), 1557-1567.

Vaikari, V. P., Park, M., Keossayan, L., MacKay, J. A., & Alachkar, H. (2020). Anti-CD99 scFv-ELP nanoworms for the treatment of acute myeloid leukemia. Nanomedicine: Nanotechnology, Biology and Medicine, 29, 102236.

Wu, J., Zhang, L., Li, H., Wu, S., & Liu, Z. (2019). Nrf2 induced cisplatin resistance in ovarian cancer by promoting CD99 expression. Biochemical and biophysical research communications, 518(4), 698-705.

Cardoso, L. C., Soares, R. D. S., Laurentino, T. D. S., Lerario, A. M., Marie, S. K., & Oba-Shinjo, S. M. (2019). CD99 expression in glioblastoma molecular subtypes and role in migration and invasion. International journal of molecular sciences, 20(5), 1137.

Pasello, M., Manara, M. C., & Scotlandi, K. (2018). CD99 at the crossroads of physiology and pathology. Journal of cell communication and signaling, 12(1), 55-68.

Çelik, H., Sciandra, M., Flashner, B., Gelmez, E., Kayraklıoğlu, N., Allegakoen, D. V., ... & Üren, A. (2018). Clofarabine inhibits Ewing sarcoma growth through a novel molecular mechanism involving direct binding to CD99. Oncogene, 37(16), 2181-2196.

Manara, M. C., Pasello, M., & Scotlandi, K. (2018). CD99: a cell surface protein with an oncojanus role in tumors. Genes, 9(3), 159.

Yeh, C. C., Chang, C. J., Twu, Y. C., Chu, C. C., Liu, B. S., Huang, J. T., ... & Yu, L. C. (2018). The molecular genetic background leading to the formation of the human erythroid-specific Xga/CD99 blood groups. Blood advances, 2(15), 1854-1864.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human CD99 ELISA Kit (CAT#: V2LY-0626-LY4388)

Human Recombinant CD99 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2991)

Human Recombinant CD99 protein, ECD, His Tag (CAT#: V2LY-0526-LY2992)

Mouse Recombinant CD99 protein, hFc Tag (CAT#: V2LY-0526-LY8085)

CD99 Matched Antibody Pair (241) (CAT#: APMAB-241LY)

Antibody Pairs

CD99 Matched Antibody Pair (241) (CAT#: APMAB-241LY)

Related Products

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12D6) (CAT#: V2LY-0725-LY277)

CD99 Matched Antibody Pair (241) (CAT#: APMAB-241LY)

Mouse Anti-CD99 Recombinant Antibody (CBT4144) (CAT#: V2LY-0625-LY1493)

Mouse Anti-CD99 Recombinant Antibody (CBYY-C1660) (CAT#: CBMAB-C3098-YY)

Human Recombinant CD99 protein, ECD, His Tag (CAT#: V2LY-0526-LY2992)

Mouse Anti-CD99 Recombinant Antibody (CBYY-C1659) (CAT#: CBMAB-C3097-YY)

Mouse Anti-CD99 Recombinant Antibody (CBT2233) (CAT#: V2LY-0625-LY1492)

Human Recombinant CD99 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2991)

Mouse Anti-CD99 Recombinant Antibody (3A10) (CAT#: CBMAB-C5624-LY)

Human CD99 ELISA Kit (CAT#: V2LY-0626-LY4388)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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