Rat Recombinant COL4A3 protein, hFc Tag (V2LY-0526-LY9304)

Name: Rat Recombinant COL4A3 protein, hFc Tag
SKU: V2LY-0526-LY9304
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

hFc Tag

Protein Construction

This product is Rat Recombinant COL4A3 protein, hFc Tag consist of Amino Acid: 1426-1670 and predicts a molecular mass of 55.2 kDa.

Molecule Mass

55.2 kDa

Sequence

Amino Acid: 1426-1670

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Collagen Type IV Alpha 3 Chain

Function

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity; these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms.

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Blood circulation Source: ProtInc
Cell adhesion Source: UniProtKB-KW
Cell surface receptor signaling pathway Source: UniProtKB
Collagen-activated tyrosine kinase receptor signaling pathway Source: Ensembl
Collagen fibril organization Source: Reactome
Endothelial cell apoptotic process Source: UniProtKB
Extracellular matrix organization Source: GO_Central
Glomerular basement membrane development Source: UniProtKB
Negative regulation of angiogenesis Source: UniProtKB
Negative regulation of cell population proliferation Source: ProtInc
Negative regulation of vascular endothelial cell proliferation Source: UniProtKB
Response to glucose Source: Ensembl
Sensory perception of sound Source: ProtInc

Cellular Location

Basement membrane. Colocalizes with COL4A4 and COL4A5 in GBM, tubular basement membrane (TBM) and synaptic basal lamina (BL).

Involvement in disease

Autoantibodies against the NC1 domain of alpha 3(IV) are found in Goodpasture syndrome, an autoimmune disease of lung and kidney.
Alport syndrome 2, autosomal recessive (ATS2):
A syndrome characterized by progressive glomerulonephritis, glomerular basement membrane defects, renal failure, sensorineural deafness and specific eye abnormalities (lenticonous and macular flecks). The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness.
Hematuria, benign familial (BFH):
An autosomal dominant condition characterized by non-progressive isolated microscopic hematuria that does not result in renal failure. It is characterized pathologically by thinning of the glomerular basement membrane.
Alport syndrome 3, autosomal dominant (ATS3):
A syndrome characterized by progressive glomerulonephritis, glomerular basement membrane defects, renal failure, sensorineural deafness and specific eye abnormalities (lenticonous and macular flecks). The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness.

PTM

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Isoform 2 contains an additional N-linked glycosylation site.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Phosphorylated. Thought to be phosphorylated by CERT, but CERT does not have kinase activity.

Alves, A. P. V., Freitas, A. B., Levi, J. E., Amorim Filho, A. G., Franco, L. A., Hoshida, M. S., ... & Carvalho, M. H. B. (2021). COL1A1, COL4A3, TIMP2 and TGFB1 polymorphisms in cervical insufficiency. Journal of Perinatal Medicine, 49(5), 553-558.

Barua, M., & Paterson, A. D. (2021). Population-based studies reveal an additive role of type IV collagen variants in hematuria and albuminuria. Pediatric Nephrology, 1-10.

Mamoor, S. (2021). COL4A3 is differentially expressed in the lymph nodes of patients with metastatic breast cancer.

Odiatis, C., Savva, I., Pieri, M., Ioannou, P., Petrou, P., Papagregoriou, G., ... & Deltas, C. (2021). A glycine substitution in the collagenous domain of Col4a3 in mice recapitulates late onset Alport syndrome. Matrix biology plus, 9, 100053.

Funk, S. D., Bayer, R. H., & Miner, J. H. (2019). Endothelial cell-specific collagen type IV-α3 expression does not rescue Alport syndrome in Col4a3−/− mice. American Journal of Physiology-Renal Physiology, 316(5), F830-F837.

Rønnow, S. R., Sand, J. M. B., Langholm, L. L., Manon-Jensen, T., Karsdal, M. A., Tal-Singer, R., ... & Leeming, D. J. (2019). Type IV collagen turnover is predictive of mortality in COPD: a comparison to fibrinogen in a prospective analysis of the ECLIPSE cohort. Respiratory research, 20(1), 1-10.

Jessen, H., Rønnow, S. R., Langholm, L., Gudmann, N. S., Karsdal, M., Manon-Jensen, T., ... & Leeming, D. J. (2019). Collagen Type IV Degradation Predicts Mortality in the Eclipse COPD Cohort. In B93. COPD: MORTALITY AND RISK PREDICTION (pp. A4001-A4001). American Thoracic Society.

Saravani, R., Yari, D., Saravani, S., & Hasanian-Langroudi, F. (2017). Correlation between the COL4A3, MMP-9, and TIMP-1 polymorphisms and risk of keratoconus. Japanese journal of ophthalmology, 61(3), 218-222.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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